Epidermal growth factor receptor dimerization monitored in live cells

Nature Biotechnology

Volumn 18, Issue 2, 2000, Pages 218-222

  Blakely, Bruce T ^a   Rossi, Fabio M V ^a   Tillotson, Bonnie ^b   Palmer, Michelle ^b   Estelles, Angeles ^a   Blau, Helen M ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b PE Biosystems   (United States)

Author keywords

EGF receptor; Protein interactions; Receptor dimerization

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; AUTOPHOSPHORYLATION; CHIMERA; DIMERIZATION; ENZYME ACTIVITY; GENETIC COMPLEMENTATION; LIGAND BINDING; MONITORING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION;

ANIMALS; BETA-GALACTOSIDASE; CELL MEMBRANE; DIMERIZATION; EPIDERMAL GROWTH FACTOR; GENETIC COMPLEMENTATION TEST; HUMANS; MICE; PROTEIN BINDING; RECEPTOR, EPIDERMAL GROWTH FACTOR; RECOMBINANT FUSION PROTEINS;

ANIMALIA;

EID: 0033965514     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/72686     Document Type: Article

References (33)

1. Schlessinger, J. & Ullrich, A. Growth factor signaling by receptor tyrosine kinases. Neuron 9, 383-391 (1992).
2. Ullrich, A. & Schlessinger, J. Signal transduction by receptors with tyrosine kinase activity. Cell 61, 203-212 (1990).
3. Weiss, F.U., Daub, H. & Ullrich, A. Novel mechanisms of RTK signal generation. Curr. Opin. Genet. Dev. 7, 80-86 (1997).
4. Fields, S. & Song, O. A novel genetic system to detect protein-protein interactions. Nature 340, 245-246 (1989).
5. 2+ based on green fluorescent proteins and calmodulin. Nature 388, 882-887 (1997).
6. Rossi, F., Charlton, C.A. & Blau, H.M. Monitoring protein-protein interactions in intact eukaryotic cells by β-galactosidase complementation. Proc. Natl. Acad. Sci. USA 94, 8405-8410 (1997).
7. Pelletier, J.N., Campbell-Valois, F.X. & Michnick, S.W. Oligomerization domain-directed reassembly of active dihydrofolate reductase from rationally designed fragments. Proc. Natl. Acad. Sci. USA 95, 12141-12146 (1998).
8. Ullmann, A., Perrin, D., Jacob, F. & Monod, J. Identification par complémentation in vitro et purification d'un segment de la β-galactosidase d'Escherichia coli. J. Mol. Biol. 12, 918-923 (1965).
9. Mohler, W.A. & Blau, H.M. Gene expression and cell fusion analyzed by lacZ complementation in mammalian cells. Proc. Natl. Acad. Sci. USA 93, 12423-12427 (1996).
10. Rossi, F.M.V., Blakely, B.T., Charlton, C.A. & Blau, H.M. in Chimeric proteins (eds Emr, S.D. & Thorner, J.) in press (Academic Press, San Diego, CA; 2000).
11. Rossi, F.M.V., Blakely, B.T. & Blau, H.M. Interaction blues: protein interactions monitored live in mammalian cells by β-galactosidase complementation. Trends Cell Biol. 10, 119-122 (2000).
12. Livneh, E. et al. Reconstitution of human epidermal growth factor receptors and its deletion mutants in cultured hamster cells. J. Biol. Chem. 261, 12490-12497 (1986).
13. Gadella, T.W., Jr. & Jovin, T.M. Oligomerization of epidermal growth factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy. A stereochemical model for tyrosine kinase receptor activation. J. Cell Biol. 129, 1543-1558 (1995).
14. Nagata, S. Apoptosis by death factor. Cell 88, 355-365 (1997).
15. Haigler, H., Ash, J.F., Singer, S.J. & Cohen, S. Visualization by fluorescence of the binding and internalization of epidermal growth factor in human carcinoma cells A-431. Proc. Natl. Acad. Sci. USA 75, 3317-3321 (1978).
16. Stoscheck, C.M. & Carpenter, G. Characterization of the metabolic turnover of epidermal growth factor receptor protein in A-431 cells. J. Cell Physiol. 120, 296-302 (1984).
17. Cowley, G.P., Smith, J.A. & Gusterson, B.A. Increased EGF receptors on human squamous carcinoma cell lines. Br. J. Cancer 53, 223-229 (1986).
18. Felder, S., LaVin, J., Ullrich, A. & Schlessinger, J. Kinetics of binding, endocytosis, and recycling of EGF receptor mutants. J. Cell Biol. 117, 203-212 (1992).
19. Kiyokawa, N., Lee, E.K., Karunagaran, D., Lin, S.Y. & Hung, M.C. Mitosis-specific negative regulation of epidermal growth factor receptor, triggered by a decrease in ligand binding and dimerization, can be overcome by overexpression of receptor. J. Biol. Chem. 272, 18656-18665 (1997).
20. Beerli, R.R. & Hynes, N.E. Epidermal growth factor-related peptides activate distinct subsets of ErbB receptors and differ in their biological activities. J. Biol. Chem. 271, 6071-6076 (1996).
21. Gill, G.N. et al. Monoclonal anti-epidermal growth factor receptor antibodies which are inhibitors of epidermal growth factor binding and antagonists of epidermal growth factor binding and antagonists of epidermal growth factor-stimulated tyrosine protein kinase activity. J. Biol. Chem 259, 7755-7760 (1984).
22. Winkler, M.E., O'Connor, L., Winget, M. & Fendly, B. Epidermal growth factor and transforming growth factor alpha bind differently to the epidermal growth factor receptor. Biochemistry 28, 6373-6378 (1989).
23. Carraway, K.L. & Cerione, R.A. Inhibition of epidermal growth factor receptor aggregation by an antibody directed against the epidermal growth factor receptor extracellular domain. J. Biol. Chem. 268, 23860-23867 (1993).
24. Yarden, Y & Schlessinger, J. Epidermal growth factor induces rapid, reversible aggregation of the purified epidermal growth factor receptor. Biochemistry 26, 1443-1451 (1987).
25. Cochet, C. et al. Demonstration of epidermal growth factor-induced receptor dimerization in living cells using a chemical covalent cross-linking agent. J. Biol. Chem. 263, 3290-3295 (1988).
26. Pollok, B.A. & Heim, R. Using GFP in FRET-based applications. Trends Cell Biol. 9, 57-60 (1999).
27. Remy, I., Wilson, I.A. & Michnick, S.W. Erythropoietin receptor activation by a ligand-induced conformation change. Science 283, 990-993 (1999).
28. Zabin, I. β-Galactosidase α-complementation. A model of protein-protein interaction. Mol. Cell. Biochem. 49, 87-96 (1982).
29. Langley, K.E. & Zabin, I. β-Galactosidase α complementation: properties of the complemented enzyme and mechanism of the complementation reaction. Biochemistry 15, 4866-4875 (1976).
30. Pegram, M. et al Inhibitory effects of combinations of HER-2/neu antibody and chemotherapeutic agents used for treatment of human breast cancers. Oncogene 18, 2241-2251 (1999).
31. Fiering, S.N. et al. Improved FACS-Gal: flow cytometric analysis and sorting of viable eukaryotic cells expressing reporter gene constructs. Cytometry 12, 291-301 (1991).
32. Nolan, G.P., Fiering, S., Nicolas, J.F. & Herzenberg, L.A. Fluorescence-activated cell analysis and sorting of viable mammalian cells based on β-D-galactosidase activity after transduction of Escherichia coli lacZ. Proc. Natl. Acad. Sci. USA 85, 2603-2607 (1988).
33. Alberti, S., Parks, D.R. & Herzenberg, L.A. A single laser method for subtraction of cell autofluorescence in flow cytometry. Cytometry 8, 114-119 (1987).