Simplified method for gene targeting vector construction

BioTechniques

Volumn 37, Issue 5, 2004, Pages 734-738

  Gasperowicz, Malgorzata ^a   Kolanczyk, Mateusz ^a   Stock, Michael ^a   Otto, Florian ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; GENOMIC DNA;

ARTICLE; DNA CLEAVAGE; DNA VECTOR; GENE TARGETING; HUMAN; MOLECULAR CLONING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION;

EID: 8444223923     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/04375bm04     Document Type: Article

References (6)

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2. Riele, H.T., E.R. Maadag, and A. Berns. 1992. Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs. Proc. Natl. Acad. Sci. USA 89:5128-5132.
3. Barnes, W.M. 1994. PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc. Natl. Acad. Sci. USA 91:2216-2220.
4. Koob, M. and W. Szybalski. 1990. Cleaving yeast and Escherichia coli genomes at a single site. Science 250:271-273.
5. Koob, M., A. Burkiewicz, J. Kur, and W. Szybalski. 1992. RecA-AC: single-site cleavage of plasmids and chromosomes at any predetermined restriction site. Nucleic Acids Res. 20:5831-5836.
6. Szybalski, W. 1997. RecA-mediated Achilles' heel cleavage. Curr. Opin. Biotechnol. 20:5831-5836.