Transcriptomal comparison of human dermal lymphatic endothelial cells ex vivo and in vitro

Physiological Genomics

Volumn 28, Issue 2, 2007, Pages 179-192

  Wick, Nikolaus ^a   Saharinen, Pipsa ^b   Saharinen, Juha ^c   Gurnhofer, Elisabeth ^a   Steiner, Carl W ^a   Raab, Ingrid ^a   Stokic, Dejan ^a   Giovanoli, Pietro ^a   Buchsbaum, Sabine ^a   Burchard, Aurea ^a   Thurner, Stefan ^a   Alitalo, Kari ^b   Kerjaschki, Dontscho ^a  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

^b Biomedicum   (Finland)

^c NATIONAL PUBLIC HEALTH INSTITUTE   (Finland)

Author keywords

In vivo; Lymphatic capillary; Transcriptome

Indexed keywords

TRANSCRIPTOME; CELL SURFACE RECEPTOR; LECTIN; MANNOSE BINDING LECTIN; MANNOSE RECEPTOR; MEMBRANE PROTEIN; PDPN PROTEIN, HUMAN; UNCLASSIFIED DRUG;

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL ISOLATION; CELL METABOLISM; COMPARATIVE STUDY; CONTROLLED STUDY; CYTOSKELETON; DNA MICROARRAY; DOWN REGULATION; ENDOTHELIUM CELL; EX VIVO STUDY; EXTRACELLULAR MATRIX; GENE ACTIVATION; GENE CONTROL; GENE IDENTIFICATION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; LYMPH VESSEL ENDOTHELIUM; MARKER GENE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUALITY CONTROL; SKIN CELL; TISSUE MICROARRAY; UNINDEXED SEQUENCE; VASCULAR ENDOTHELIUM; BIOLOGICAL MODEL; CYTOLOGY; DERMIS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETICS; IMMUNOHISTOCHEMISTRY; METABOLISM; METHODOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; THEORETICAL MODEL;

CELLS, CULTURED; DERMIS; ENDOTHELIAL CELLS; ENDOTHELIUM, LYMPHATIC; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; HUMANS; IMMUNOHISTOCHEMISTRY; LECTINS, C-TYPE; MANNOSE-BINDING LECTINS; MEMBRANE GLYCOPROTEINS; MODELS, GENETIC; MODELS, THEORETICAL; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; RECEPTORS, CELL SURFACE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSCRIPTION, GENETIC;

EID: 33947714588     PISSN: 10948341     EISSN: 15312267     Source Type: Journal    
DOI: 10.1152/physiolgenomics.00037.2006     Document Type: Article

References (30)

1. Breiteneder-Geleff S, Soleiman A, Kowalski H, Horvat R, Amann G, Kriehuber E, Diem K, Weninger W, Tschachler E, Alitalo K, Kerjaschki D. Angiosarcomas express mixed endothelial phenotypes of blood and lymphatic capillaries: podoplanin as a specific marker for lymphatic endothelium. Am J Pathol 154: 385-394, 1999.
2. Durr E, Yu J, Krasinska KM, Carver LA, Yates JR, Testa JE, Oh P, Schnitzer JE. Direct proteomic mapping of the lung microvascular endothelial cell surface in vivo and in cell culture. Nat Biotechnol 22: 985-992, 2004.
3. Engerman RL, Pfaffenbach D, Davis MD. Cell turnover of capillaries. Lab Invest 17: 738-743, 1967.
4. Hirakawa S, Hong YK, Harvey N, Schacht V, Matsuda K, Libermann T, Detmar M. Identification of vascular lineage-specific genes by transcriptional profiling of isolated blood vascular and lymphatic endothelial cells. Am J Pathol 162: 575-586, 2003.
5. Hobson B, Denekamp J. Endothelial proliferation in tumours and normal tissues: continuous labelling studies. Br J Cancer 49: 405-413, 1984.
6. Jackson DG. Biology of the lymphatic marker LYVE-1 and applications in research into lymphatic trafficking and lymphangiogenesis. APMIS 112: 526-538, 2004.
7. Kalt R, Okubo Y, Benisch C, Nagavarapu U, Herron GS, Geleff S, Soleiman A, Schoppmann SF. Telomerase-immortalized lymphatic and blood vessel endothelial cells are functionally stable and retain their lineage specificity. Microcirculation 11: 261-269, 2004.
8. Karkkainen MJ, Alitalo K. Lymphatic endothelial regulation, lymphoedema, and lymph node metastasis. Semin Cell Dev Biol 13: 9-18, 2002.
9. Karkkainen MJ, Haiko P, Sainio K, Partanen J, Taipale J, Petrova TV, Jeltsch M, Jackson DG, Talikka M, Rauvala H, Betsholtz C, Alitalo K. Vascular endothelial growth factor C is required for sprouting of the first lymphatic vessels from embryonic veins. Nat Immun 5: 74-80, 2004.
10. Kerjaschki D, Regele HM, Moosberger I, Nagy-Bojarski K, Watschinger B, Soleiman A, Birner P, Krieger S, Hovorka A, Silberhumer G, Laakkonen P, Petrova T, Langer B, Raab I. Lymphatic neoangiogenesis in human kidney transplants is associated with immunologically active lymphocytic infiltrates. J Am Soc Nephrol 15: 603-612, 2004.
11. Kriehuber E, Breiteneder-Geleff S, Groeger M, Soleiman A, Schoppmann SF, Stingl G, Kerjaschki D, Maurer D. Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages. J Exp Med 194: 797-808, 2001.
12. Makinen T, Veikkola T, Mustjoki S, Karpanen T, Catimel B, Nice EC, Wise L, Mercer A, Kowalski H, Kerjaschki D, Stacker SA, Achen MG, Alitalo K. Isolated lymphatic endothelial cells transduce growth, survival and migratory signals via the VEGF-C/D receptor VEGFR-3. EMBO J 20: 4762-4773, 2001.
13. Nachman RL, Jaffe EA. Endothelial cell culture: beginnings of modern vascular biology. J Clin Invest 114: 1037-1040, 2004.
14. Pakkasjarvi N, Gentile M, Saharinen J, Honkanen J, Herva R, Peltonen L, Kestila M. Indicative oligodendrocyte dysfunction in spinal cords of human fetuses suffering from a lethal motoneuron disease. J Neurobiol 65: 269-281, 2005.
15. Petrova TV, Karpanen T, Norrmen C, Mellor R, Tamakoshi T, Finegold D, Ferrell R, Kerjaschki D, Mortimer P, Yla-Herttuala S, Miura N, Alitalo K. Defective valves and abnormal mural cell recruitment underlie lymphatic vascular failure in lymphedema distichiasis. Nat Med 10: 974-981, 2004.
16. Petrova TV, Makinen T, Makela TP, Saarela J, Virtanen I, Ferrell RE, Finegold DN, Kerjaschki D, Yla-Herttuala S, Alitalo K. Lymphatic endothelial reprogramming of vascular endothelial cells by the Prox-1 homeobox transcription factor. EMBO J 21: 4593-4599, 2002.
17. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29: e45, 2001.
18. Podgrabinska S, Braun P, Velasco P, Kloos B, Pepper MS, Skobe M. Molecular characterization of lymphatic endothelial cells. Proc Natl Acad Sci USA 99: 16069-16074, 2002.
19. Sauter B, Foedinger D, Sterniczky B, Wolff K, Rappersberger K. Immunoelectron microscopic characterization of human dermal lymphatic microvascular endothelial cells. Differential expression of CD31, CD34, and type IV collagen with lymphatic endothelial cells vs blood capillary endothelial cells in normal human skin, lymphangioma, and hemangioma in situ. J Histochem Cytochem 46: 165-176, 1998.
20. Schacht V, Ramirez MI, Hong YK, Hirakawa S, Feng D, Harvey N, Williams M, Dvorak AM, Dvorak HF, Oliver G, Detmar M. T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema. EMBO J 22: 3546-3556, 2003.
21. Schoppmann SF, Birner P, Studer P, Breiteneder-Geleff S. Lymphatic microvessel density and lymphovascular invasion assessed by anti-podoplanin immunostaining in human breast cancer. Anticancer Res 21: 2351-2355, 2001.
22. Skobe M, Hawighorst T, Jackson DG, Prevo R, Janes L, Velasco P, Riccardi L, Alitalo K, Claffey K, Detmar M. Induction of tumor lymphangiogenesis by VEGF-C promotes breast cancer metastasis. Nat Med 7: 192-198, 2001.
23. Stacker SA, Caesar C, Baldwin ME, Thornton GE, Williams RA, Prevo R, Jackson DG, Nishikawa S, Kubo H, Achen MG. VEGF-D promotes the metastatic spread of tumor cells via the lymphatics. Nat Med 7: 186-191, 2001.
24. Swartz MA, Boardman KC Jr. The role of interstitial stress in lymphatic function and lymphangiogenesis. Ann NY Acad Sci 979: 197-210; discussion 229-134, 2002.
25. The Tumor Analysis Best Practices Working Group. Expression profiling-best practices for data generation and interpretation in clinical trials. Nat Rev Genet 5: 229-237, 2004.
26. Vollmar B, Wolf B, Siegmund S, Katsen AD, Menger MD. Lymph vessel expansion and function in the development of hepatic fibrosis and cirrhosis. Am J Pathol 151: 169-175, 1997.
27. Weber E, Rossi A, Solito R, Sacchi G, Agliano M, Gerli R. Focal adhesion molecules expression and fibrillin deposition by lymphatic and blood vessel endothelial cells in culture. Microvasc Res 64: 47-55, 2002.
28. Wick N, Bruck J, Gurnhofer E, Steiner CW, Giovanoli P, Kerjaschki D, Thurner S. Nonuniform hybridization: a potential source of error in oligonucleotide-chip experiments with low amounts of starting material. Diagn Mol Pathol 13: 151-159, 2004.
29. Wigle JT, Oliver G. Prox1 function is required for the development of the murine lymphatic system. Cell 98: 769-778, 1999.
30. Zhang X, Groopman JE, Wang JF. Extracellular matrix regulates endothelial functions through interaction of VEGFR-3 and integrin alpha(5)beta(1). J Cell Physiol 202: 205-214, 2005.