Cloning, expression, purification, and characterization of a novel epoxide hydrolase from Aspergillus niger SQ-6

Protein Expression and Purification

Volumn 53, Issue 2, 2007, Pages 239-246

  Liu, Yanbin ^a,b   Wu, Sheng ^a   Wang, Jianjun ^a   Yang, Liu ^a   Sun, Wanru ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Aspergillus niger; Cloning; Epoxide hydrolase; Inverse PCR; Protein purification

Indexed keywords

EPOXIDE HYDROLASE; FUNGAL DNA; METAL; RECOMBINANT PROTEIN; SOLVENT;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS NIGER; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; EXON; FUNGAL GENE; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; PHYLOGENY; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; TEMPERATURE;

AMINO ACID SEQUENCE; ASPERGILLUS NIGER; BASE SEQUENCE; CLONING, MOLECULAR; DNA, FUNGAL; EPOXIDE HYDROLASES; ESCHERICHIA COLI; EXONS; GENE EXPRESSION; GENES, FUNGAL; HYDROGEN-ION CONCENTRATION; KINETICS; METALS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHYLOGENY; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SOLVENTS; SUBSTRATE SPECIFICITY; TEMPERATURE;

ASPERGILLUS NIGER; ESCHERICHIA COLI;

EID: 33947389159     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.06.017     Document Type: Article

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