An improved protocol for generation of immuno-potent dendritic cells through direct electroporation of CD14+ monocytes

Journal of Immunological Methods

Volumn 321, Issue 1-2, 2007, Pages 94-106

  Milano, Francesca ^a   van Baal, Jantine W P M ^a   Rygiel, Agnieszka M ^a   Bergman, Jacques J G H M ^a   Van Deventer, Sander J H ^a   Kapsenberg, Martien L ^a   Peppelenbosch, Maikel P ^b   Krishnadath, Kausilia K ^a  

^a ACADEMIC MEDICAL CENTER   (Netherlands)

^b UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

Author keywords

APC; Dendritic cells; Gene therapy; GFP; Immunotherapy; RNA electroporation; Vaccination

Indexed keywords

B7 ANTIGEN; CD14 ANTIGEN; CD83 ANTIGEN; CD86 ANTIGEN; CHEMOKINE RECEPTOR CCR7; GAMMA INTERFERON; GREEN FLUORESCENT PROTEIN; INTERLEUKIN 12P70; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; RNA;

ADULT; ANTIGEN PRESENTATION; ARTICLE; BLOOD; CD8+ T LYMPHOCYTE; CELL ISOLATION; CELL MATURATION; CLINICAL PROTOCOL; CONTROLLED STUDY; DENDRITIC CELL; ELECTROPORATION; FEMALE; HUMAN; HUMAN CELL; IMMUNOPOTENTIATION; IMMUNOSTIMULATION; LYMPHOCYTE ACTIVATION; MALE; MONOCYTE; NORMAL HUMAN; PRIORITY JOURNAL;

ADULT; ANTIGENS, CD; ANTIGENS, CD14; CD4-CD8 RATIO; CD8-POSITIVE T-LYMPHOCYTES; CELL COMMUNICATION; CELL DIFFERENTIATION; CELL SURVIVAL; CELLS, CULTURED; COCULTURE TECHNIQUES; DENDRITIC CELLS; ELECTROPORATION; FEMALE; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HISTOCOMPATIBILITY ANTIGENS CLASS I; HUMANS; INTERFERON TYPE II; INTERLEUKIN-10; INTERLEUKIN-12; LYMPHOCYTE ACTIVATION; MALE; MONOCYTES; RECEPTORS, CHEMOKINE; RNA; TIME FACTORS; TRANSFECTION;

EID: 33947318768     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2007.01.004     Document Type: Article

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