IVET-based identification of virulence factors in Vibrio vulnificus MO6-24/O

Journal of Microbiology and Biotechnology

Volumn 17, Issue 2, 2007, Pages 234-243

  Lee, Ko Eun ^a   Bang, Ji Sun ^a   Baek, Chang Ho ^a,c   Park, Dae Kyun ^a   Hwang, Won ^a   Choi, Sang Ho ^b   Kim, Kun Soo ^a  

^a Sogang University   (South Korea)

^b Seoul National University   (South Korea)

^c ARIZONA STATE UNIVERSITY   (United States)

Author keywords

IVET; TolC; Vibrio vulnificus; Virulence factor

Indexed keywords

ADENOSINE DEAMINASE; BACTERIAL DNA; BETA GALACTOSIDASE; CARRIER PROTEIN; CHROMATE TRANSPORT PROTEIN A; CYTOSINE DEAMINASE; GLYCEROL 3 PHOSPHATE ACYLTRANSFERASE; HEMAGGLUTININ; KANAMYCIN; LEXA PROTEIN; MANNOSE PHOSPHATE ISOMERASE; METHIONINE SYNTHASE; METHIONINE SYNTHASE I; PEPTIDASE; TOLC PROTEIN; UNCLASSIFIED DRUG; VIRULENCE FACTOR;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BIOFILM; CELL GROWTH; CELL MUTANT; COLONY FORMING UNIT; CONTROLLED STUDY; CYTOTOXICITY; DNA SEQUENCE; FEMALE; GENE EXPRESSION PROFILING; GENE FREQUENCY; GENE IDENTIFICATION; GENE ISOLATION; GRAM NEGATIVE INFECTION; GROWTH RATE; HUMAN; HUMAN CELL; IN VIVO STUDY; MOLECULAR CLONING; MOUSE; NONHUMAN; OPEN READING FRAME; PROMOTER REGION; REPORTER GENE; SEQUENCE HOMOLOGY; VIBRIO VULNIFICUS; WILD TYPE;

AMINO ACID SEQUENCE; ANIMALS; ARTIFICIAL GENE FUSION; BIOFILMS; FEMALE; GENE LIBRARY; GENOME, BACTERIAL; HELA CELLS; HUMANS; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; VIBRIO VULNIFICUS; VIRULENCE FACTORS;

MUS; VIBRIO VULNIFICUS;

EID: 33947270307     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (35)

1. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database searching programs. Nucleic Acid Res. 25: 3389-3402.
2. Baek, C. H. and K. S. Kim. 2003. Promoterless lacZ- and aph- Based reporter vectors for in vivo expression technology. J. Micribiol. Biotechnol. 13: 972-980.
3. Bina, J. E. and J. J. Mekalanos. 2001. Fibrio Cholerae tolC is required for bile resistance and colonization. Infect. Immun. 69: 4681-4685.
4. Camilli, A., D. Beattie, and J. J. Mekalanos. 1995. Use of recombinase gene fusions to identify ribrio cholerae genes induced during infection. Mol. Microbiol. 8: 143-157.
5. Cangelosi, G. A., E. A. Best, C. Martinetti, and E. W. Nester. 1991. Genetic analysis of Agrobacterium. Methods Enzymol. 204: 384-397.
6. Chen, C. Y. et al. 2003. Comparative genome analysis of Vibrio vulnificus, a marine pathogen. Genome Res. 13: 2577-2587.
7. Chiang, S. L., J. J. Mekalanos, and D. W. Holden. 1999. In vivo genetic analysis of bacterial virulence. Annu. Rev. Microbiol. 53: 129-154.
8. De Lorenzo, V., M. Herrero, U. Jakubzik, and K. N. Timmis. 1990. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. J. Bacteriol. 172: 6568-6572.
9. Gray, L. D. and A. S. Kreger. 1985. Purification and characterization of extracellular cytolysin produced by Vibrio vulnificus. Infect. Immune. 48: 62-72.
10. Heidelberg, J. F. et al. 2002. DNA sequence of both chromosomes of the cholerae pathogen Vibrio cholerae. Nature 406: 477-483.
11. Hunt, M. L., D. J. Boucher, J. D. Boyce, and B. Adler. 2001. In vivo-expressed genes of Pasteurella multocida. Infect. Iimmun. 69: 3004-3012.
12. Janakiraman, A. and J. M. Slauch. 2000. The putative iron transport system SitABCD encoded on SPI1 is required for full virulence of Salmonella typhimurium. Mol. Microbiol. 35: 1146-1155.
13. Jeong, H. S., J. E. Rhee, J. H. Lee, H. K. Choi, D. I. Kim, M. H. Lee, S. J. Park, and S. H. Choi. 2003 Identification of Vibrio vulnificus lrp and Its Influence on Survival under Various Stresses. J. Microbiol. Biotechnol 13: 159-163.
14. Kaniga, K., I. Dellor, and G. R. Cornelis. 1991. A wide-host-range suicide vector for improving reverse genetics in Gram-negative bacteria: In activation of the blaA gene of Yersinia enterocolitica. Gene 20: 137-141.
15. Keen, N. T., S. Takami, and D. Kobayashi, and D. Trollinger. 1988. Improved broad-host range plasmids for DNA cloning in Gram-negative bacteria. Gene 70: 191-197
16. Lee, S. H., S. M. Butler, and A. Camilli. 2001. Selection for in vivo regulators of bacterial virulence. Proc. Nalt. Acad. Sci. USA 98: 6889-6894.
17. Lim, M. S., M. H. Lee, J. H. Lee, H. M. Ju, N. Y. Park, H. S. Jeong, J. E. Rhee, and S. H. Choi. 2005. Identification and Characterization of the Vibrio vulnificus malPQ Operon. J. Microbiol. Biotechnol. 15: 616-625.
18. Litwin, C. M., T. W. Rayback, and J. Skinner. 1996. Role of catechol siderophore synthesis in Vibrio vulnificus virulence. Infect. Immun. 64: 2934-2938.
19. Lowe, A. M., D. T. Beattie, and R. L. Deresiewicz. 1998. Identification of novel staphylococcal virulence genes by in vivo expression technology. Mol. Microbiol. 27: 967-976.
20. Mahan, M. J., J. M. Slauch, and J. J. Mekalanos. 1993. Selection of bacterial virulence genes that are specifically induced in host tissue. Science 259: 686-688.
21. Makino, K. et al. 2003. Genome sequences of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of Vibrio cholerae. Lancet 361: 743-749.
22. McDougald, D., S, A. Rice, and S. Kjelleberg. 2000. The marine pathogen Vibrio vulnificus encodes a putative homologue of the Vibrio harveyi regulatory gene, luxR: A genetic and phylogenetic comparoson. Gene 248: 213-221.
23. Milton, D. L., R. O'Toole, P. Horstedt, and H. Wolf-Watz. 1996. Flagellin A in essential for the virulence of Vibrio angullarum. J. Bacteriol. 178: 1310-1319.
24. Park, K. J., S. H. H. Kim, M. G. Kim, D. H. Chung, S. D. Ha, K. S. Kim, D. J. Jahng, and K. H. Lee. 2004. Functional Complementation of Escherichia coli by the rpoS Gene of the Foodborne Pathogenic Vibrio vulnificus. J. Microbiol. Biotechnol. 14: 1063-1066.
25. Parsot, C., E. Taxman, and J. J. Mekalanos. 1991. ToxR regulates the production of lipoproteins and the expression of serum resistance in Vibrio cholerae. Proc. Natl. Acad. Sci. USA 88: 1641-1645.
26. Reddy, G. P., U. Hayat, C. Abeygunawardana, C. Fox, A. C. Wright, D. R. Maneval, Jr., C. A. Bush, and J. G Morris, Jr. 1992. Purification and determination of the structure of capsular polysaccharide of Vibrio vulnificus MO6-24. J. Bacterial. 174: 2620-2630.
27. Rhee, J. E., H. M. Ju, U. R. Park, B. C, Park, and S. H. Choi. 2004. Identification of the Vibrio vulnificus cadC and evaluation of its role in acid tolerance. J. Microbiol. Biotechnol. 14: 1093-1098.
28. Sambrook, J. and D. W. Russell. 2001. Molecular cloning: A laboratory manual, 3rd ed. Cold Spring Harbor Press, Cold Spring Harbor, N.Y.
29. Simon, R., U. Priefer, and A. Puhler. 1983. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Bio/Technology 1: 784-791.
30. Simpson, L. M., V. K. White, S. F. Zane, and J. D. Oliver. 1987. Correlation between virulence and colony morphology in Vibrio vulnificus. Infect. Immun. 55: 269-272.
31. Sutcliff, I. C. and D. J. Harrington. 2002. Pattern searches for the identification of putative lipoprotein genes in Gram-positive bacterial genomes. Microbiology 148: 2065-2077
32. Testa, J., L. W. Daniel, and A. S. Kreger. 1984. Extracellular phospholipase A2 and lysophospholipase produced by Vibrio vulnificus. Infect. Immun. 45: 777-793.
33. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acid Research 22: 4673-4680.
34. Wang, J., A. Mushegian, S. Lory, and S. Jin. 1996. Large-scale isolation of candidate virulence genes of Pseudomonas aeruginosa by in vivo selection. Proc. Natl. Acad. Sci. USA 93: 10434-10438.
35. Young, G. M. and V. L. Miller. 1997. Identification of novel chromosomal loci affecting Yersinia enterocolitica pathogenesis. Mol. Micriobiol. 25: 319-328.