Pros and cons: Cryo-electron microscopic evaluation of block faces versus cryo-sections from frozen-hydrated skin specimens prepared by different techniques

Journal of Microscopy

Volumn 225, Issue 2, 2007, Pages 201-207

  Richter, T ^a   Biel, S S ^b   Sattler, M ^b   Wenck, H ^b   Wittern, K P ^b   Wiesendanger, R ^c   Wepf, R ^d  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b Beiersdoft AG   (Germany)

^c UNIVERSITY OF HAMBURG   (Germany)

^d ETH ZURICH   (Switzerland)

Author keywords

Block face; Cryo section; Cryo SEM; Frozen hydrated; Skin barrier; Stratum corneum

Indexed keywords

BIOLOGICAL MATERIALS; ELECTRONS; FREEZING; HEAVY METALS; SCANNING ELECTRON MICROSCOPY;

BIOLOGICAL SAMPLES; BLOCK FACE; CRYO-SECTION; CRYO-SEM; FROZEN HYDRATED; HIGH-PRESSURE FREEZING; MICROSCOPIC EVALUATION; PLUNGE FREEZING; SKIN BARRIER; STRATUM CORNEUM;

HYDRATION;

CARBON; HEAVY METAL; WATER;

ARTICLE; COMPARATIVE STUDY; CRYOELECTRON MICROSCOPY; ELECTRON BEAM; FROZEN SECTION; HUMAN; HUMAN TISSUE; HYDRATION; MORPHOLOGY; PRESSURE MEASUREMENT; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SCANNING ELECTRON MICROSCOPE; SCANNING ELECTRON MICROSCOPY; SKIN; STATISTICAL ANALYSIS; STEREOLOGY; STRUCTURE ANALYSIS; ULTRAMICROTOMY;

EID: 33847755185     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2007.01732.x     Document Type: Article

References (27)

1. Al-Amoudi, A., Dubochet, J., Gnaegi, H., Luethi, W. & Studer, D. (2003) An oscillating cryo-knife reduces cutting-induced deformation of vitreous ultrathin sections. J. Microsc. 212, 26-33.
2. Al-Amoudi, A., Norlen, L.P.O. & Dubochet, J. (2004) Cryo-electron microscopy of vitreous sections of native biological cells and tissues. J. Struct. Biol. 148, 131-135.
3. Caspers, P.J., Lucassen, G.W., Carter, E.A., Bruining, H.A. & Puppels, G.J. (2001) In vivo confocal Raman microspectroscopy of the skin: noninvasive determination of molecular concentration profiles. J. Invest. Dermatol. 116, 434-442.
4. Denk, W., Horstmann, H. (2004) Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure. PLoS 2, 1900-1909.
5. Droste, M.S., Biel, S.S., Terstegen, L., Wittern, K.-P., Wenck, H. & Wepf, R. (2005) Noninvasive measurement of cell volume changes by negative staining. J Biomed Opt 10, 064017, 1-10.
6. Dubochet, J. & McDowall, A.W. (1984) Science of biological specimen preparation for microsc. microanal. Pfefferkorn Conference (ed. by S. A. Bhatt, G. H. Haggis, J.-P. Revel and T. Barnard). Scanning Microscopy Int., Chicago.
7. Echlin, P. (1992) Low-Temperature Microscopy and Analysis. Plenum Pub Corp, New York.
8. Erk, I., Nicolas, G., Caroff, A. & Lepault, J. (1998) Electron microscopy of frozen biological objects: a study using cryosectioning and cryosubstition. J. Microsc. 189, 236-248.
9. Hallegot, P., Minondo, A.M. & Fiat, F. (1999) Cryo-techniques applied to stratum corneum with description of a new sample holder for cryoscanning electron microscopy of freeze-fractured samples. J. Microsc. 196, 35-39.
10. Heymann, J.A.W., Hayles, M., Gestmann, I., Giannuzzi, L.A., Lich, B. & Subramaniam, S. (2006) Site-specific 3D imaging of cells and tissues with a dual beam microscope. J. Struct. Biol. 155, 63-73.
11. Hohenberg, H., Tobler, M. & Mueller, M. (1996) High-pressure freezing of tissue obtained by fine-needle biopsy. J. Microsc. 183, 133-139.
12. Howard, V. & Reed, M.G. (2005). Unbiased Stereology : Three-Dimensional Measurement In Microscopy. pp.1-93. BIOS Scientific Publishers, Taylor & Francis Group, New York.
13. Michel, M., Hillman, T. & Mueller, M. (1991) Cryosectioning of plant material frozen at high pressure. J. Microsc. 163, 3-18.
14. Michel, M., Gnägi, H. & Mueller, M. (1992) Diamonds are a cryosectioner's best friend. J. Microsc. 166, 43-56.
15. Hsieh, C., Marko, M., Frank, J. & Mannella, C.A. (2002) Electron tomographic analysis of frozen-hydrated tissue sections. J. Struct. Biol. 138, 63-73.
16. Mohr, H. & Riehle, U. (1968) Snap freezing under high-pressure: a new fixation technique for freeze-etching. Proceedings of the 4th Eur. Reg. Conference Electron Microscopy, Rome Vol. 2 (ed. by D. Bocciarelli),pp. 33-34.
17. Norlen, L. & Al-Amoudi, A. (2004) Stratum Corneum keratin structure, function, and formation: the cubic rod-packing and membrane templating model. J. Invest. Dermatol. 123, 715-732.
18. Pfeiffer, S., Vielhaber, G., Vietzke, J.P., Wittern, K.-P., Hintze, U. & Wepf, R. (2000) High-pressure freezing provides new information on human epidermis: simultaneous protein antigen and lamellar lipid structure preservation. Study on human epidermis by cryoimmobilization. J. Invest. Dermatol. 114, 1030-1038.
19. Richter, T., Peuckert, C., Sattler, M., et al. (2004) Dead but highly dynamic - the stratum corneum is divided into three hydration zones. Skin Pharmacol. Physiol. 17, 246-257.
20. Ritter, M., Henry, D., Wiesner, S., Pfeiffer, S. & Wepf, R. (1999) A versatile high-vacuum cryo-transfer for cryo-FESEM, cryo-SPM and other imaging techniques. Microsc. Microanal. (ed. by D. E. Johnson, A. K. Christensen, M. A. O'Keefe, et al.), pp. 424-425. Springer-Verlag, Berlin.
21. Sartori, N., Bednar, J. & Dubochet, J. (1996) Electron-beam-induced amorphization of ice III or IX obtained by high-pressure freezing. J. Microsc. 182, 163-168.
22. Sartori Blanc, N., Studer, D., Ruhl, K. & Dubochet, J. (1998) Electron beam-induced changes in vitreous sections of biological samples. J. Microsc. 192, 194-201.
23. Studer, D., Michel, M. & Mueller, M. (1989) High pressure freezing comes of age. Scanning Microsc. Suppl. 3, 253-268.
24. Walther, P. & Mueller, M. (1999) Biological ultrastructure as revealed by high resolution cryo-SEM of block faces after cryo-sectioning. J. Microsc 196, 279-287.
25. Walther, P., Wehrli, E., Hermann, R. and Mueller, M. (1995) Double-layer coating for high-resolution low-temperature scanning electron microscopy. J. Microsc. 179, 229-237.
26. Warner, R.R., Myers, M.C. & Taylor, D.A. (1988) Electron probe analysis of human skin: determination of the water concentration profile. J. Invest. Dermatol. 90, 218-224.
27. Zhang, P., Bos, E., Heymann, J., Gnaegi, H., Kessel, M., Peters, P.J. & Subramaniam, S. (2004) Direct visualization of receptor arrays in frozen-hydrated sections and plunge-frozen specimens of E. coli engineered to overproduce the chemotaxis receptor Tsr. J. Microsc. 216, 76-83.