Cell cycle phase dependent productivity of a recombinant Chinese hamster ovary cell line

Cytotechnology

Volumn 52, Issue 1, 2006, Pages 55-69

  Dutton, Roshni L ^a   Scharer, Jeno ^b   Moo Young, Murray ^b  

^a BioProcess Assist (BPA) Ltd   (Canada)

^b UNIVERSITY OF WATERLOO   (Canada)

Author keywords

Cell cycle associated protein productivity; Chinese hamster ovary (CHO); DHFR; Regulation of expression; Specific productivity; Tissue plasminogen activator (tPA)

Indexed keywords

DIHYDROFOLATE REDUCTASE; TISSUE PLASMINOGEN ACTIVATOR;

ANIMAL CELL; ARTICLE; BACTERIUM CULTURE; CELL CYCLE G1 PHASE; CELL CYCLE G2 PHASE; CELL CYCLE M PHASE; CELL CYCLE PHASE; CELL CYCLE S PHASE; CELL FUNCTION; CELL LINE; CELL POPULATION; CELL SUBPOPULATION; CELL VIABILITY; CHO CELL; CONTROLLED STUDY; FLOW CYTOMETRY; GENE AMPLIFICATION; NONHUMAN; PRODUCTIVITY; PROMOTER REGION;

ANIMALIA; CRICETULUS GRISEUS;

EID: 33847295859     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-006-9041-4     Document Type: Article

References (54)

1. Aggeler J, Kapp LN, Tseng SCG, Werb Z (1982) Regulation of protein secretion in Chinese Hamster Ovary cells by cell cycle position and cell density. Exp Cell Res 139:275-283
2. Al-Fageeh MB, Marchant RJ, Carden MJ, Smales CM (2006) The cold-shock response in cultured mammalian cells: harnessing the response for the improvement of recombinant protein production. Biotechnol Bioeng 93:829-835
3. Altamirano C, Cairo JJ, Godia F (2001) Decoupling cell growth and product formation in chinese hamster ovary cells through metabolic control. Biotechnol Bioeng 76:351-360
4. Andersen DC, Bridges T, Gawlitzek M, Hoy C (2000) Multiple cell culture factors can affect the glycosylation of Asn-184 in CHO-produced tissue-type plasminogen activator. Biotech Bioeng 70:25-31
5. Assaraf YG, Seamer LC, Schmike RT (1989) Characterization by flow cytometry of fluorescein-methotrexate transport in Chinese Hamster Ovary cells. Cytometry 10:50-55
6. Balcarcel RR, Stephanopoulos G (2001) Rapamycin reduces hybridoma cell death and enhances monoclonal antibody production. Biotechnol Bioeng 76:1-10
7. Banik GG, Todd PW, Kompala DS (1996) Foreign protein expression from S phase specific promoters in continuous cultures of recombinant CHO cells. Cytotechnology 22:179-184
8. Bjorklund M, Taipale M, Varjosalo M, Saharinen J, Lahdenpera J, Taipale J (2006) Identification of pathways regulating cell size and cell-cycle progression by RNAi. Nature 439:1009-1013
9. Cartwright T (1994) Animal cells as bioreactors. Cambridge University Press, Cambridge, UK, 184 p
10. Carvalhal AV, Santos SS, Calado J, Haury M, Carrondo MJT (2003) Cell growth arrest by nucleotides, nucleosides and bases as a tool for improved production of recombinant proteins. Biotechnol Prog 19:69-83
11. Chevalot I, Visvikis A, Nabet P, Engasser J-M, Marc A (1994) Production of a membrane-bound protein, the human gamma-glutamyl transferase, by CHO cells cultivated on microcarriers, in aggregates and in suspension. Cytotechnology 16:121-129
12. Croughan MS, Wang DIC (1990) Reversible removal and hydrodynamic phenomena in CHO microcarrier cultures. Biotechnol Bioeng 36:316-319
13. Darzynkiewicz A, Hoffmann MK (1994) CD4 engagement induces Fas antigen-dependent apoptosis of T cells in vivo. Eur J Immunol 24:1549-1552
14. de Boer L, Gray PP, Sunstrom N-A (2004) Enhanced productivity of G1 phase Chinese hamster ovary cells using the GADD153 promoter. Biotechnol Lett 26:61-65
15. 2 and osmolality: intracellular pH, cell size, apoptosis, and metabolism. Biotechnol Bioeng 77:369-380
16. Dutton RL, Scharer JM, Moo-Young M (1998) Descriptive parameter evaluation in mammalian cell culture. Cytotechnology 26:139-152
17. Dutton RL, Scharer JM, Moo-Young M (1999) Hybridoma growth and productivity: effects of conditioned medium and of inoculum size. Cytotechnology 29:1-10
18. Fann CH, Guirgis F, Chen G, Lao MS, Piret JM (2000) Limitations to the amplification and stability of human tissue-type plasminogen activator expression by Chinese hamster ovary cells. Biotechnol Bioeng 69:204-212
19. Fox SR, Tan HK, Tan MC, Wong SCNC, Yap MGS, Wang DIC (2005) A detailed understanding of the enhanced hypothermic productivity of interferon- gamma by Chinese-hamster ovary cells. Biotechnol Appl Biochem 41:255-264
20. Freedman RB, Greenall C, Jenkins N, Tuite MF (1995) Protein folding in the secretory pathway of animal cells. Cytotechnology 18:77-82
21. Fussenegger M, Mazur X, Bailey JE (1997) A novel cytostatic process enhances the productivity of Chinese hamster ovary cells. Biotechnol Bioeng 55:927-939
22. Genentech Inc (1988) US Patent 4,766,075
23. Glacken MW, Fleischaker RJ, Sinskey AJ (1983) Large-scale production of mammalian cells and their products: engineering principles and barriers to scale-up. Biochem Eng III 413:355-372
24. Gu MB, Todd P, Kompala DS (1993) Foreign gene expression (β-galactosidase) during the cell cycle phases in recombinant CHO cells. Biotechnol Bioeng 42:1113-1123
25. Hayduk EJ, Lee KH (2005) Cytochalasin D can improve heterologous protein productivity in adherent Chinese hamster ovary cells. Biotechnol Bioeng 90:354-364
26. Hayter PM, Curling EMA, Gould ML, Baines AJ, Jenkins N, Salmon I, Strange PG, Bull AT (1993) The effect of the dilution rate on CHO cell physiology and recombinant interferon-γ production of glucose-limited chemostat culture. Biotechnol Bioeng 42:1077-1085
27. Hayward GL, Dutton RL, Zhang Z, Scharer JM, Moo-Young M (1998) Thickness shear mode acoustic assay for plasminogen activators. Anal Commun 35:25-27
28. Hendrick V, Winnepenninckx P, Abdelkafi C, Vandeputte O, Cherlet M, Marique T, Renemann G, Loa A, Kretzmer G, Werenne J (2001) Increased productivity of recombinant tissular plasminogen activator (t-PA) by butyrate and shift of temperature: a cell cycle phases analysis. Cytotechnology 36:71-83
29. Ibarra N, Watanabe S, Bi J-X, Shuttleworth J, Al-Rubeai M (2003) Modulation of cell cycle for enhancement of antibody productivity in perfusion culture of NS0 cells. Biotechnol Prog 19:224-228
30. Inaba T, Mori J, Ohmura M, Tani H, Kato Y, Tomizawa K, Kato T, Ihara T, Sato I, Ueda S (1998) Recombinant porcine follicle stimulating hormone produced in baculovirus-insect cells induces rat ovulation in vivo and gene expression of tissue plasminogen activator in vitro. Res Vet Sci 64:25-29
31. James DC, Goldman MH, Hoare M, Jenkins N, Oliver RW, Green BN, Freedman RB (1996) Posttranslational processing of recombinant human interferon-gamma in animal expression systems. Protein Sci 5:331-340
32. Jensen LJ, Jensen TS, de Lichtenberg U, Brunak S, Bork P (2006) Co-evolution of transcriptional and post-translational cell-cycle regulation. Nature 443:594-597
33. Johnson LF (1984) Expression of dihydrofolate reductase and thymidylate synthase genes in mammalian cells. In: Stein GS, Stein JL (eds) Recombinant DNA and cell proliferation. Academic Press Inc, USA, pp 26-46
34. Kaufmann H, Mazur X, Fussenegger M, Bailey JE (1999) Influence of low temperature on productivity, proteome, and protein phosphorylation of CHO cells. Biotechnol Bioeng 63:573-582
35. Kaufman RJ, Bertino JR, Schimke RT (1978) Quantitation of dihydrofolate reductase in individual parental and methotrexate-resistant murine cells. J Biol Chem 254:5852-5860
36. Kubbies M, Stockinger H (1990) Cell cycle-dependent DHFR and t-PA production in contransfected, MTX-amplified CHO cells revealed by dual-laser flow cytometry. Exp Cell Res 188:267-271
37. Lakhotia S, Bauer KD, Papoutsakis ET (1992) Damaging agitation intensities increase DNA synthesis rate and alter cell-cycle phase distributions of CHO cells. Biotechnol Bioeng 40:978-990
38. Leelavatcharamas V, Emery AN, Al-Rubeai M (1999) Use of cell cycle analysis to characterize growth and interferon-γ production in perfusion culture of CHO cells. Cytotechnology 30:59-69
39. Lloyd DR, Holmes P, Jackson LP, Emery AN, Al-Rubeai M (2000) Relationship between cell size, cell cycle, and specific recombinant protein productivity. Cytotechnology 4:57-70
40. Loskutoff DJ, Paul D (1978) Intracellular plasminogen activator in growing and quiescent cells. J Cell Physiol 97:9-16
41. Mariani BD, Slate DL, Schimke RT (1981) S phase-specific synthesis of dihydrofolate reductase in Chinese hamster ovary cells. Proc Natl Acad Sci USA 78:4985-4989
42. Milner AE, Wang H, Gregory CD (1996) Analysis of apoptosis by flow cytometry. In: Al-Rubeai M, Emery AN (eds) Flow cytometry applications in cell culture. Marcel Dekker Inc, New York, pp 193-209
43. Ohta T, Kimble M, Essner R, Kofron M, Kuriyama R (1996) Cell cycle-dependent expression of the CHO2 antigen, a minus-end directed kinesin-like motor in mammalian cells. Protoplasma 190:131-140
44. Phillips HJ (1973) Dye exclusion tests for cell viability. In: Kruse Jr PF, Patterson Jr MK (eds) Tissue culture: methods and applications. Academic Press, New York, pp 406-408
45. Rouf SA, Moo-Young M, Chisti Y (1996) Tissue-type plasminogen activator: characteristics, applications and production technology. Biotechnol Adv 14:239-266
46. Scott FM, de Serrano VS, Catellino FJ (1987) Appearance of plasminogen activator activity during a synchronous cycle of a rat adenocarcinoma cell line, PA-III. Exp Cell Res 169:39-46
47. Shen X, Minoura H, Yoshida T, Toyoda N (1997) Changes in ovarian expression of tissue-type plasminogen activator and plasminogen activator inhibitor type-1 messenger ribonucleic acids during ovulation in rat. Endocr J 44:341-348
48. Sherlock G (2006) Complex evolution. Nature 443:513-515
49. Smith JA, Martin L (1973) Do cells cycle? Proc Natl Acad Sci USA 70:1263-1267
50. Sun Z, Zhou R, Liang S, McNeeley KM, Sharfstein ST (2004) Hyperosmotic stress in murine hybridoma cells: effects on antibody transcription, translation, posttranslational processing, and the cell cycle. Biotechnol Prog 20:576-589
51. Sunstrom N-A, Sugiyono, Hunt S, Bailey C, Baig M, Sleigh M, Gray P (2000) Regulated autocrine growth of CHO cells. Cytotechnology 34:39-46
52. Warner TG (1999) Enhancing therapeutic glycoprotein production in Chinese hamster ovary cells by metabolic engineering endogenous gene control with antisense DNA and gene targeting. Glycobiology 9:841-850
53. Yoon SK, Song JY, Lee GM (2003) Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells. Biotechnol Bioeng 82:289-298
54. Yoon SK, Choi SL, Song JY, Lee GM (2005) Effect of culture pH on erythropoietin production by Chinese hamster ovary cells grown in suspension at 32.5 and 37.0°C. Biotechnol Bioeng 89:345-356