Functional analysis of Kluyveromyces lactis carboxylic acids permeases: Heterologous expression of KlJEN1 and KlJEN2 genes

Current Genetics

Volumn 51, Issue 3, 2007, Pages 161-169

  Queirós, Odília ^a,c   Pereira, Leonor ^b,c   Paiva, Sandra ^b   Moradas Ferreira, Pedro ^c,d   Casal, Margarida ^b  

^a UNIVERSITY INSTITUTE OF HEALTH SCIENCES IUCS   (Portugal)

^b UNIVERSITY OF MINHO   (Portugal)

^c UNIVERSITY OF PORTO   (Portugal)

^d UNIVERSITY OF PORTO   (Portugal)

Author keywords

Carboxylate transport; GFP; Heterologous expression; Kluyveromyces lactis; Yeast

Indexed keywords

CARBOXYLIC ACID; DICARBOXYLATE TRANSPORTER; FUNGAL ENZYME; GREEN FLUORESCENT PROTEIN; LACTIC ACID; MALIC ACID; MONOCARBOXYLATE TRANSPORTER; PERMEASE; SUCCINIC ACID;

ARTICLE; CELL MEMBRANE; CONTROLLED STUDY; FUNCTIONAL PROTEOMICS; FUNGAL GENE; FUNGAL GENETICS; GENETIC CODE; HETEROLOGOUS EXPRESSION; KLUYVEROMYCES; MEMBRANE TRANSPORT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; TRANSPORT KINETICS;

CARBOXYLIC ACIDS; DICARBOXYLIC ACID TRANSPORTERS; GENE EXPRESSION REGULATION, FUNGAL; KLUYVEROMYCES; LACTIC ACID; MEMBRANE TRANSPORT PROTEINS; MONOCARBOXYLIC ACID TRANSPORTERS; PYRUVIC ACID; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, NUCLEIC ACID; SYMPORTERS;

KLUYVEROMYCES LACTIS; SACCHAROMYCES CEREVISIAE;

EID: 33847198627     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-006-0107-9     Document Type: Article

References (42)

1. Andrade RP, Casal M (2001) Expression of the lactate permease gene JEN1 from the yeast Saccharomyces cerevisiae. Fungal Genet Biol 32:105-111
2. Ausubel FA, Brent R, Kingston D, Moore D, Seidman JG, Smith JA, Struhl K (1998) In: Current protocols in molecular biology. Wiley, New York, pp 4.9.1-4.9.11
3. Breunig KD, Bolotin-Fukuhara M, Bianchi MM, Bourgarel D, Falcone C, Ferrero I, Frontali L, Goffrini P, Krijger JJ, Mazzoni C, Milkowski C, Steensma HY, Wesolowski-Louvel M (2000) Regulation of primary carbon metabolism in Kluyveromyces lactis. Enzyme Microb Technol 26:771-780
4. Casal M, Cardoso H, Leão C (1996) Mechanisms regulating the transport of acetic acid in Saccharomyces cerevisiae. Microbiology 142:1385-1390
5. Casal M, Leão C (1995) Utilization of short-chain monocarboxylic acids by the yeast Torulaspora delbrueckii: Specificity of the transport systems and their regulation. Biochim Biophys Acta 1267:122-130
6. Casal M, Paiva S, Andrade RP, Gancedo C, Leão C (1999) The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1. J Bacteriol 181:2620-2623
7. Cássio F, Leão C (1993) A comparative study on the transport of L(-)malic acid and other short-chain carboxylic acids in the yeast Candida utilis: Evidence for a general organic acid permease. Yeast 9:743-752
8. Cássio F, Leão C, van Uden N (1987) Transport of lactate and other short-chain monocarboxylates in the yeast Saccharomyces cerevisiae. Appl Environ Microbiol 53:509-513
9. Cárte-Real M, Leão C (1989) Transport of L-malic acid and other dicarboxylic acids in the yeast Candida sphaerica. Appl Microbiol Biotechnol 31:551-555
10. Cárte-Real M, Leão C (1990) Transport of malic acid and other dicarboxylic acids in the yeast Hansenula anomala. Appl Environ Microbiol 56:1109-1113
11. De Deken RH (1966) The Crabtree effect: A regulatory system in yeast. J Gen Microbiol 44:149-156
12. Ferrero I, Rossi C, Landini MP, Puglisi PP (1978) Role of the mithocondrial protein synthesis in the catabolite repression of the petite-negative yeast Kluyveromyces lactis. Biochem Biophys Res Commun 80:340-348
13. Fonseca A, Spencer-Martins I, van Uden N (1991) Transport of lactic acid in Kluyveromyces marxianus: Evidence for a monocarboxylate uniport. Yeast 7:775-780
14. Gerós H, Cássio F, Leão C (2000) Utilization and transport of acetic acid in Dekkera anomala and their implications on the survival of the yeast in acidic environments. J Food Prot 63(1):96-101
15. Goffrini P, Algeri AA, Donnini C, Wésolowski-Louvel M, Ferrero I (1989) RAG1 and RAG2: Nuclear genes involved in the dependence/ independence on mitochondrial respiratory function for growth on sugars. Yeast 5(2):99-106
16. Grobler J, Bauer F, Subden RE, van Vuuren HJJ (1995) The mae1 gene of Schizosaccharomyces pombe encodes a permease for malate and other C4 dicarboxylic acids. Yeast 11:1485-1491
17. Güldener U, Heck S, Fiedler T, Beinhauer J, Hegemann JH (1996) A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res 24:2519-2524
18. Leão C, van Uden N (1986) Transport of lactate and other short chain monocarboxylates in the yeast Candida utilis. Appl Microbiol Biotechnol 23:389-393
19. Lodi T, Fontanesi F, Ferrero I, Donnini C (2004) Carboxylic acids permeases in yeast: Two genes in Kluyveromyces lactis. Gene 339:111-119
20. Milkowski C, Krampe S, Weirich J, Hasse V, Boles E, Breunig KD (2001) Feedback regulation of glucose transporter gene transcription in Kluyveromyces lactis by glucose uptake. J Bacteriol 183(18):5223-5229
21. Mumberg D, Mailer R, Funk M (1995) Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156:119-122
22. Osothsilp C, Subden RE (1986) Malate transport in Schizosaccharomyces pombe. J Bacteriol 168:1439-1443
23. Paiva S, Devaux F, Barbosa S, Jacq C, Casal M (2004) Ady2p is essencial for the acetate permease activity in the yeast Saccharomyces cerevisiae. Yeast 21(3):201-210
24. Paiva S, Kruckeberg AL, Casal M (2002) Utilization of green fluorescent protein as a marker for studying the expression and turnover of the monocarboxylate permease Jen1p of Saccharomyces cerevisiae. Biochem J 363(Pt 3):737-744
25. Prior C, Mamessier P, Fukuhara H, Chen XJ, Wésolowski-Louvel M (1993) The hexokinase gene is required for transcriptional regulation of the glucose transporter gene RAG1 in Kluyveromyces lactis. Mol Cell Biol 13(7):3882-3889
26. Queirós O (2002) Transporte e utilização de ácidos dicarboxílicos nas leveduras Kluyveromyces sp. e Saccharomyces cerevisiae: Uma abordagem fisiológica, bioquímica e genética. Ph.D. Thesis, University of Minho
27. Queirós O, Casal M, Althoff S, Moradas-Ferreira P, Leão C (1998) Isolation and characterization of Kluyveromyces marxianus mutants deficient in malate transport. Yeast 14:401-407
28. Queirós O, Paiva S, Moradas-Ferreira P, Casal M (2003) Molecular and physiological characterization of monocarboxylic acids permeases in the yeast Kluyveromyces lactis. Yeast 20:S237
29. Radler F (1993) Yeasts - metabolism of organic acids. In: Fleet GH (ed) Wine microbiology and biotechnology. Harwood Academic Publishers, Switerzland, pp 165-182
30. Salmon JM (1987) L-malic acid permeation in resting cells of anaerobically grown Saccharomyces cerevisiae. Biochim Biophys Acta 901:30-34
31. Sambrook J, Fritisch EF, Maniatis T (1989) Molecular cloning: A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, New York
32. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463-5467
33. Snoek IS, Steensma HY (2006) Why does Kluyveromyces lactis not grow under anaerobic conditions? Comparison of essential anaerobic genes of Saccharomyces cerevisiae with the Kluyveromyces lactis genome. FEMS Yeast Res 6(3):393-403
34. Soares-Silva I, Paiva S, Kötter P, Entian K-D, Casal M (2004) Disruption of JEN1 from Candida albicans impairs the transport of lactate. Mol Membr Biol 21:401-411
35. Soares-Silva I, Schüller D, Andrade RP, Baltazar F, Cássio F, Casal M (2003) Functional expression of the lactate permease Jen1p of Saccharomyces cerevisisiae in Pichia pastoris. Biochem J 376(Pt 3):781-787
36. Sousa MJ, Miranda L, Côrte-Real M, Leão C (1996) Transport of acetic acid in Zygosaccharomyces bailii: Effects of ethanol and their implications on the resistance of the yeast to acidic environments. Appl Environ Microbiol 62:3152-3157
37. Sousa MJ, Mota M, Leão C (1992) Transport of malic acid in the yeast Schizosaccharomyces pombe: Evidence for a proton-dicarboxylate symport. Yeast 8:1025-1031
38. Steensma HY (2003) Removal of dominant markers from the Kluyveromyces lactis genome using Cre/loxP system. In: Wolf K (ed) Non-convencional yeasts in genetics biochemistry and biotechnology. Springer, Berlin Heidelberg New York pp 175-178
39. Thomas BJ, Rothstein R (1989) Elevated recombination rates in transcriptionally active DNA. Cell 56:619-630
40. Weirich J, Goffrini P, Kuger P, Ferrero I, Breunig KD (1997) Influence of mutations in hexose-transporter genes on glucose repression in Kluyveromyces lactis. Eur J Biochem 249(1):248-257
41. Wésolowski-Louvel M, Breunig KD, Fukuhara H (1996) Kluyveromyces lactis. In: Wolf K (ed) Nonconventional yeasts in biotechnology, a handbook. Springer, Berlin Heidelberg New York, pp 139-201
42. Zmijewski MJJr, MacQuillan AM (1975) Dual effects of glucose on dicarboxylic acids transport in Kluyveromyces lactis. Can J Microbiol 21:473-480