Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12

Protein Expression and Purification

Volumn 53, Issue 1, 2007, Pages 97-103

  Wang, Jinxia ^a,b   Tan, Haidong ^a  

^a Kent)   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Enzyme kinetics; Escherichia coli K12; Malic enzyme; Over expression

Indexed keywords

ISOPROPYL THIOGALACTOSIDE; MALATE DEHYDROGENASE; MALATE DEHYDROGENASE (OXALOACETATE DECARBOXYLATING) (NAD+); MALATE DEHYDROGENASE-(OXALOACETATE-DECARBOXYLATING) (NAD+); NICOTINAMIDE ADENINE DINUCLEOTIDE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI K 12; GENE EXPRESSION; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR WEIGHT; PH; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; SOLUBILITY;

CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI K12; GENE EXPRESSION; GENETIC VECTORS; HYDROGEN-ION CONCENTRATION; ISOPROPYL THIOGALACTOSIDE; KINETICS; MALATE DEHYDROGENASE; MOLECULAR WEIGHT; NAD; PLASMIDS; RECOMBINANT PROTEINS; SOLUBILITY; TRANSFORMATION, GENETIC;

ESCHERICHIA COLI;

EID: 33847130448     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.11.017     Document Type: Article

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