Assessing the data quality in predictive toxicology using a panel of cell lines and cytotoxicity assays

Analytical Biochemistry

Volumn 362, Issue 2, 2007, Pages 221-228

  Pohjala, Leena ^a   Tammela, Päivi ^a   Samanta, Swapan K ^a   Yli Kauhaluoma, Jari ^a   Vuorela, Pia ^a,b  

^a UNIVERSITY OF HELSINKI   (Finland)

^b ÅBO AKADEMI UNIVERSITY   (Finland)

Author keywords

ATP assay; Cell viability assay; Data quality; LDH leakage assay; Triazoles; WST 1 reduction assay

Indexed keywords

CELL CULTURE; MAMMALS; TOXICITY;

AUTOMATED ENVIRONMENTS; BIOCHEMICAL CHARACTERISTICS; CELL VIABILITY ASSAYS; CYTOTOXICITY ASSAYS; DATA QUALITY; MAMMALIAN CELL LINES; SENSITIVITY PROFILES; TRIAZOLES;

CELLS;

1,2,4 TRIAZOLE DERIVATIVE; 2 (4 IODOPHENYL) 3 (4 NITROPHENYL) 5 (2,4 DISULFOPHENYL) 2H TETRAZOLIUM; ADENOSINE TRIPHOSPHATE; CAMPTOTHECIN; FLUOROURACIL; GRAMICIDIN; LACTATE DEHYDROGENASE; POLYMYXIN B; TETRAZOLIUM; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL LINE; CELL STRAIN; CELL STRAIN BHK; CELL STRAIN CACO 2; CELL VIABILITY; CYTOTOXICITY TEST; DATA ANALYSIS; HUMAN; HUMAN CELL; IC 50; LIVER CELL CULTURE; NONHUMAN; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; REDUCTION; SENSITIVITY AND SPECIFICITY;

MAMMALIA;

EID: 33846922033     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.12.038     Document Type: Article

References (30)

1. Kola L., and Landis J. Can the pharmaceutical industry reduce attrition rates?. Nat. Rev. Drug Discov. 3 (2004) 711-715
2. Pritchard J.F., Jurima-Romet J.M., Reimer M.I., Mortimer E., Rolfe B., and Cayen M.N. Making better drugs: Decision gates in non-clinical drug development. Nat. Rev. Drug Discov. 2 (2003) 542-553
3. European Commission, The new chemicals legislation REACH (2004), http://ec.europa.eu/enterprise/reach/index_en.htm.
4. Wilson A.P. Cytotoxicity and viability assays. In: Masters J. (Ed). Animal Cell Culture: A Practical Approach (2000), Oxford University Press, New York 175-218
5. Dorn A., Hermann F., Ebneth A., Bothmann H., Trube G., and Apfel K.ChristensenC. Evaluation of a high-throughput fluorescence assay method for hERG potassium channel inhibition. J. Biomol. Screen. 10 (2005) 339-347
6. Kim M.J., Kim H., Cha I.J., Park I.S., Shon J.H., Liu K.H., and Shi J.G. High-throughput screening of inhibitory potential of nine cytochrome P450 enzymes in vitro using liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom. 19 (2005) 2651-2658
7. Schultz T.W., Cromin M.T.D., Walker J.D., and Aptula A.O. Quantitative structure-activity relationships in toxicology: A historical perspective. J. Mol. Struct. 622 (2001) 1-22
8. Gubbels-van Hal W.M., Blaauboer B.J., Barentsen H.M., Hoitink M.A., Meerts I.A., and van der Hoeven J.C. An alternative approach for the safety evaluation of new and existing chemicals: an exercise in integrated testing. Regul. Toxicol. Pharmacol. 42 (2005) 284-295
9. Simon-Hettich B., Rothfuss A., and Steger-Hartmann T. Use of computer-assisted prediction of toxic effects of chemical substances. Toxicology 224 (2006) 156-162
10. Fotakis G., and Timbrell J.A. In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT, and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol. Lett. 160 (2005) 171-177
11. Hamid R., Rotshteyn Y., Rabadi L., Parikh R., and Bullock P. Comparison of Alamar blue and MTT assays for high through-put screening. Toxicol. In Vitro 18 (2004) 703-710
12. Holst C.M., and Oredsson S.M. Comparison of three cytotoxicity tests in the evaluation of the cytotoxicity of a spermine analogue on human breast cancer cell lines. Toxicol. In Vitro 19 (2005) 379-387
13. Lappalainen K., Jääskeläinen I., Syrjänen K., Urtti A., and Syrjänen S. Comparison of cell proliferation and toxicity assays using two cationic liposomes. Pharm. Res. 11 (1994) 1127-1131
14. Mueller H., Kassack M.U., and Wiese M. Comparison of the usefulness of the MTT, ATP, and calcein assays to predict the potency of cytotoxic agents in various human cancer cell lines. J. Biomol. Screen. 9 (2004) 506-515
15. Samanta S.K., and Yli-Kauhaluoma J. Polymer-supported 1,3-oxazolium-5-olates: synthesis of 1,2,4-triazoles. J. Comb. Chem. 7 (2005) 142-146
16. Korzeniewski C., and Callewaert D.M. An enzyme-release assay for natural cytotoxicity. J. Immunol. Methods 64 (1983) 313-320
17. Ishiyama M., Tominaga H., Shiga M., Sasamoto R., Ohkora Y., Ueno K., and Watanabe M. Novel cell proliferation and cytotoxicity assays using a tetrazolium salt that produces a water-soluble formazan dye. In Vitro Toxicol. 8 (1995) 187-190
18. Zhang J.H., Chun T.D.Y., and Oldenburg K.R. A simple statistical parameter for use in evaluation and validation of high-throughput screening assays. J. Biomol. Screen. 4 (1999) 67-73
19. Bollini S., Herbst J.J., Gaughan G.T., Verdoorn T.A., Ditta J., Dupowchik G.M., and Vinitsky A. High-throughput fluorescence polarization method fpr identification of FRBP12 ligands. J. Biomol. Screen. 7 (2002) 526-530
20. Vellonen K.S., Honkakoski P., and Urtti A. Substrates and inhibitors of efflux proteins interfere with the MTT assay in cells and may lead to underestimation of drug toxicity. Eur. J. Pharm. Sci. 23 (2004) 181-188
21. Crouch S.P., Kozlowsk R., Slater K.J., and Fletcher I. The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity. J. Immunol. Methods 160 (1993) 81-88
22. Hidalgo I.J., Raub T.J., and Borchardt R.T. Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology 96 (1989) 736-749
23. Meaney C., Florea B.I., Ehrhardt C., Schafer U.F., and Lehr C.M. Bronchial epithelial cell cultures. In: Lehr C.M. (Ed). Cell Culture Models of Biological Barriers: In-Vitro Test Systems for Drug Absorption and Delivery (2002), Taylor & Francis, London 211-228
24. Stoker M., and Macpherson I. Syrian hamster fibroblast cell line BHK21 and its derivatives. Nature 203 (1964) 1355-1359
25. Nakabayanshi H., Taketa K., Miyano K., Yamane T., and Sato J. Growth of human hepatoma cell line with differentiated functions in chemically defined medium. Cancer Res. 42 (1982) 3858-3863
26. Miret S., de Groene E.M., and Klaffke W. Comparison of in vitro assays of cellular toxicity in the human hepatic cell line HepG2. J. Biomol. Screen. 11 (2006) 184-193
27. Jung L.L., and Zamboni W.C. Cellular, pharmacokinetic, and pharmacodynamic aspects of response to camptothecins: can we improve it?. Drug Resist. Updat. 4 (2001) 273-288
28. Moridani M.Y., Siraki A., Chevaldina T., Scobie H., and O'Brien P.J. Quantitative structure toxicity relationships for catechols in isolated rat hepatocytes. Chem. Biol. Interact. 147 (2004) 297-307
29. Siraki A.G., Chan T.S., and O'Brien P.J. Application of the quantitative structure-toxicity relationships for the comparison of the cytotoxicity of 14 p-benzoquinone congeners in primary cultured rat hepatocytes versus PC12 cells. Toxicol. Sci. 8 (2004) 148-159
30. Roy K., and Leonard J.T. QSAR by LEER model of cytotoxicity data of anti-HIV 5-phenyl-1-phenylamino-1H-imidazole derivatives using principal component factor analysis and genetic function approximation. Bioorg. Med. Chem. 13 (2005) 2967-2973