Metallothionein and hsp70 expression in HepG2 cells after prolonged cadmium exposure

Toxicology in Vitro

Volumn 21, Issue 2, 2007, Pages 314-319

  Urani, C ^a   Melchioretto, P ^a   Canevali, C ^a   Morazzoni, F ^a   Gribaldo, L ^b  

^a UNIVERSITY OF MILANO BICOCCA   (Italy)

^b JOINT RESEARCH CENTRE   (Italy)

Author keywords

Cadmium; HepG2; Hsp70; Metallothioneins

Indexed keywords

CADMIUM CHLORIDE; HEAT SHOCK PROTEIN 70; METALLOTHIONEIN;

ADSORPTION KINETICS; ARTICLE; CELL DAMAGE; CONTROLLED STUDY; CYTOTOXICITY; EXPOSURE; HEPATOMA CELL; HUMAN; HUMAN CELL; LIVER PROTECTION; LIVER TOXICITY; METAL TOLERANCE; PROTEIN EXPRESSION; STRESS;

CADMIUM CHLORIDE; CELL LINE, TUMOR; CELL SURVIVAL; HSP70 HEAT-SHOCK PROTEINS; HUMANS; LIVER; METALLOTHIONEIN;

EID: 33846782886     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2006.08.014     Document Type: Article

References (24)

1. Andrews G.K. Regulation of metallothionein gene expression by oxidative stress and metal ions. Biochemical Pharmacology 59 (2000) 95-104
2. Beyersmann D. Effects of carcinogenic metals on gene expression. Toxicology Letters 127 (2002) 63-68
3. Beyersmann D., and Hechtenberg S. Cadmium, gene regulation and cellular signalling in mammalian cells. Toxicology and Applied Pharmacology 144 (1997) 247-261
4. Bonham R.T., Fine M.R., Pollock F.M., and Shelden E.A. Hsp27, hsp70, and metallothionein in MDCK and LLC-PK1 renal epithelial cells: effects of prolonged exposure to cadmium. Toxicology and Applied Pharmacology 191 (2003) 63-73
5. Carmichael J., DeGraff W.G., Gadzar A.F., Minna J.D., and Mitchell J. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Research 47 (1987) 936-942
6. DelRaso N.J., Foy B.D., Gearhart J.M., and Frazier J.M. Cadmium uptake kinetics in rat hepatocytes: correction for albumin binding. Toxicological Sciences 72 (2003) 19-30
7. Farzaneh P., Allameh A., Pratt S., Moore N., Travis L., Gottschlag E., Kind C., and Fry J. Increased heat shock protein 70 expression following toxicant-mediated cytotoxicity: a ubiquitous marker of toxicant exposure?. ATLA 33 (2005) 105-110
8. Fatur T., Tušek M., Falnoga I., Ščančar J., Lah T.T., and Filipič M. DNA damage and metallothionein synthesis in human hepatoma cells (HepG2) exposed to cadmium. Food and Chemical Toxicology 40 (2002) 1069-1076
9. International Agency for Research on Cancer: IARC Monographs on the Evaluation of Carcinogenic Risk to Humans: Berillium, Cadmium, Mercury and Exposures I the Glass Industry. IARC Scientific Publications no. 58. IARC, Lyon, France, 1993, pp. 119-238.
10. Klaassen C.D., Liu J., and Supratim C. Metallothionein: an intracellular protein to protect against cadmium toxicity. Annual Review of Pharmacology and Toxicology 39 (1999) 267-294
11. Knasmüller S., Mersch-Sundermann V., Kevekordes S., Darroudi F., Huber W.W., Hoelzl C., Bichler J., and Majer B.J. Use of human-derived liver cell lines for the detection of environmental and dietary genotoxicants: current state of knowledge. Toxicology 198 (2004) 315-328
12. Lowry O.H., Rosenbrought N.J., Farr A.L., and Randall R.L. Protein measurement with the folin phenol red reagent. Journal of Biological Chemistry 193 (1951) 265-275
13. Mayer M.P., and Bukau B. Hsp70 chaperones: cellular functions and molecular mechanism. Cellular and Molecular Life Sciences 62 (2005) 670-684
14. Mizzen C.A., Cartel N.J., Yu W.H., Fraser P.E., and McLachlan D. Sensitive detection of metallothioneins-1, -2 and -3 in tissue homogenates by immunoblotting: a method for enhanced membrane transfer and retention. Journal of Biochemical and Biophysical Methods 32 (1996) 77-83
15. Nordberg M. Metallothioneins: historical review and state of knowledge. Talanta 46 (1998) 243-254
16. Santoro M.G. Heat shock factors and the control of the stress response. Biochemical Pharmacology 59 (2000) 55-63
17. 2. Environmental Health Perspectives 107 (1999) 887-893
18. Somji S., Sens M.A., Garrett S.H., Gurel V., Todd J.H., and Sens D.A. Expression of hsp 90 in human kidney and proximal tubule cells exposed to heat, sodium arsenite and cadmium chloride. Toxicology Letters 133 (2002) 241-254
19. Sousa V., Escobar M.d.C., Gómez-Quiroz L., Bucio L., Hernández E., Chávez Cossio E., and Gutiérrez-Ruiz Ma.C. Acute cadmium exposure enhances AP-1 DNA binding and induces cytokines expression and heat shock protein 70 in HepG2 cells. Toxicology 197 (2004) 213-228
20. Tchounwou P.B., Ishaque A.B., and Scneider J. Cytotoxicity and transcriptional activation of stress genes in human liver carcinoma cells (HepG2) exposed to cadmium chloride. Molecular and Cellular Biochemistry 222 (2001) 21-28
21. Urani C., Melchioretto P., Morazzoni F., Canevali C., and Camatini M. Copper and zinc uptake and hsp70 expression in HepG2 cells. Toxicology in Vitro 15 (2001) 497-502
22. Urani C., Melchioretto P., Carnevali C., and Crosta G.F. Cytotoxicity and induction of protective mechanisms in HepG2 exposed to cadmium. Toxicology in Vitro 19 (2005) 887-892
23. Wang S., and Shi X. Molecular mechanisms of metal toxicity and carcinogenicity. Molecular and Cellular Biochemistry 222 (2001) 3-9
24. Wilkening S., Sthal F., and Bader A. Comparison of primary human hepatocytes and hepatoma cell line HepG2 with regard to their biotransformation properties. Drug Metabolism and Disposition 31 (2003) 1035-1042