Mutational analysis revealed that conservation of hepatitis B virus reverse transcriptase residue 306 (rtP306) is crucial for encapsidation of pregenomic RNA

FEBS Letters

Volumn 581, Issue 3, 2007, Pages 558-564

  Wang, Yong Xiang ^a   Xu, Xiang ^b   Luo, Cheng ^c   Ma, Zhang Mei ^a   Jiang, Hua Liang ^c   Ding, Jian Ping ^b   Wen, Yu Mei ^a  

^a BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

^b INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^c SHANGHAI INSTITUTE OF MATERIA MEDICA   (China)

Author keywords

Encapsidation; Hepatitis B virus; Replication; Reverse transcriptase

Indexed keywords

PREGENOMIC RNA; RNA DIRECTED DNA POLYMERASE; UNCLASSIFIED DRUG; VIRUS RNA;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME STRUCTURE; GENETIC CONSERVATION; HEPATITIS B VIRUS; HUMAN; HUMAN CELL; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; STRAIN DIFFERENCE; VIRUS CAPSID; VIRUS CELL INTERACTION; VIRUS REPLICATION;

AMINO ACID SUBSTITUTION; BASE SEQUENCE; CELL LINE; CONSERVED SEQUENCE; DNA, VIRAL; GENETIC COMPLEMENTATION TEST; HEPATITIS B VIRUS; HUMANS; MUTAGENESIS, SITE-DIRECTED; PROLINE; RECOMBINANT PROTEINS; RNA, VIRAL; RNA-DIRECTED DNA POLYMERASE; TRANSFECTION; VIRUS ASSEMBLY; VIRUS REPLICATION;

DNA VIRUSES; HEPATITIS B VIRUS;

EID: 33846496283     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2007.01.024     Document Type: Article

References (37)

1. Ganem D., and Prince A.M. Hepatitis B virus infection - natural history and clinical consequences. N. Engl. J. Med. 350 (2004) 1118-1129
2. Beasley R.P. Hepatitis B virus. The major etiology of hepatocellular carcinoma. Cancer 61 (1988) 1942-1956
3. Beasley R.P., Hwang L.Y., Lin C.C., and Chien C.S. Hepatocellular carcinoma and hepatitis B virus. A prospective study of 22 707 men in Taiwan. Lancet 2 (1981) 1129-1133
4. Seeger C., and Mason W.S. Hepatitis B virus biology. Microbiol. Mol. Biol. Rev. 64 (2000) 51-68
5. Radziwill G., Tucker W., and Schaller H. Mutational analysis of the hepatitis B virus P gene product: domain structure and RNase H activity. J. Virol. 64 (1990) 613-620
6. Nassal M., and Rieger A. A bulged region of the hepatitis B virus RNA encapsidation signal contains the replication origin for discontinuous first-strand DNA synthesis. J. Virol. 70 (1996) 2764-2773
7. Pollack J.R., and Ganem D. Site-specific RNA binding by a hepatitis B virus reverse transcriptase initiates two distinct reactions: RNA packaging and DNA synthesis. J. Virol. 68 (1994) 5579-5587
8. Wang G.H., and Seeger C. Novel mechanism for reverse transcription in hepatitis B viruses. J. Virol. 67 (1993) 6507-6512
9. Weber M., Bronsema V., Bartos H., Bosserhoff A., Bartenschlager R., and Schaller H. Hepadnavirus P protein utilizes a tyrosine residue in the TP domain to prime reverse transcription. J. Virol. 68 (1994) 2994-2999
10. Zoulim F., and Seeger C. Reverse transcription in hepatitis B viruses is primed by a tyrosine residue of the polymerase. J. Virol. 68 (1994) 6-13
11. Bartenschlager R., Junker-Niepmann M., and Schaller H. The P gene product of hepatitis B virus is required as a structural component for genomic RNA encapsidation. J. Virol. 64 (1990) 5324-5332
12. Bartenschlager R., and Schaller H. Hepadnaviral assembly is initiated by polymerase binding to the encapsidation signal in the viral RNA genome. Embo J. 11 (1992) 3413-3420
13. Hirsch R.C., Lavine J.E., Chang L.J., Varmus H.E., and Ganem D. Polymerase gene products of hepatitis B viruses are required for genomic RNA packaging as wel as for reverse transcription. Nature 344 (1990) 552-555
14. Hu J., and Seeger C. Hsp90 is required for the activity of a hepatitis B virus reverse transcriptase. Proc. Natl. Acad. Sci. USA 93 (1996) 1060-1064
15. Hu J., Toft D.O., and Seeger C. Hepadnavirus assembly and reverse transcription require a multi-component chaperone complex which is incorporated into nucleocapsids. Embo J. 16 (1997) 59-68
16. Knaus T., and Nassal M. The encapsidation signal on the hepatitis B virus RNA pregenome forms a stem-loop structure that is critical for its function. Nucleic Acids Res. 21 (1993) 3967-3975
17. Roychoudhury S., Faruqi A.F., and Shih C. Pregenomic RNA encapsidation analysis of eleven missense and nonsense polymerase mutants of human hepatitis B virus. J. Virol. 65 (1991) 3617-3624
18. Arauz-Ruiz P., Norder H., Robertson B.H., and Magnius L.O. Genotype H: a new Amerindian genotype of hepatitis B virus revealed in Central America. J. Gen. Virol. 83 (2002) 2059-2073
19. Chu C.J., and Lok A.S. Clinical significance of hepatitis B virus genotypes. Hepatology 35 (2002) 1274-1276
20. Fung S.K., and Lok A.S. Hepatitis B virus genotypes: do they play a role in the outcome of HBV infection?. Hepatology 40 (2004) 790-792
21. Lin X., Ma Z.M., Yao X., He L.F., Yuan Z.H., Ding J.P., and Wen Y.M. Substitution of proline 306 in the reverse transcriptase domain of hepatitis B virus regulates replication. J. Gen. Virol. 86 (2005) 85-90
22. Lin X., Yuan Z.H., Wu L., Ding J.P., and Wen Y.M. A single amino acid in the reverse transcriptase domain of hepatitis B virus affects virus replication efficiency. J. Virol. 75 (2001) 11827-11833
23. Hui E.K., Yi Y.S., and Lo S.J. Hepatitis B viral core proteins with an N-terminal extension can assemble into core-like particles but cannot be enveloped. J. Gen. Virol. 80 Pt 10 (1999) 2647-2659
24. Sambrook J., and Russell D.W. Molecular Cloning: a Laboratory Manual. 3rd ed. (2001), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
25. Gazina E.V., Fielding J.E., Lin B., and Anderson D.A. Core protein phosphorylation modulates pregenomic RNA encapsidation to different extents in human and duck hepatitis B viruses. J. Virol. 74 (2000) 4721-4728
26. Hu J., Flores D., Toft D., Wang X., and Nguyen D. Requirement of heat shock protein 90 for human hepatitis B virus reverse transcriptase function. J. Virol. 78 (2004) 13122-13131
27. Yu M., and Summers J. Multiple functions of capsid protein phosphorylation in duck hepatitis B virus replication. J. Virol. 68 (1994) 4341-4348
28. Yuan T.T., Sahu G.K., Whitehead W.E., Greenberg R., and Shih C. The mechanism of an immature secretion phenotype of a highly frequent naturally occurring missense mutation at codon 97 of human hepatitis B virus core antigen. J. Virol. 73 (1999) 5731-5740
29. Doitsh G., and Shaul Y. Enhancer I predominance in hepatitis B virus gene expression. Mol. Cell Biol. 24 (2004) 1799-1808
30. Bock C.T., Malek N.P., Tillmann H.L., Manns M.P., and Trautwein C. The enhancer I core region contributes to the replication level of hepatitis B virus in vivo and in vitro. J. Virol. 74 (2000) 2193-2202
31. Seeger C., Leber E.H., Wiens L.K., and Hu J. Mutagenesis of a hepatitis B virus reverse transcriptase yields temperature-sensitive virus. Virology 222 (1996) 430-439
32. Hu J., and Seeger C. Expression and characterization of hepadnavirus reverse transcriptases. Methods Enzymol. 275 (1996) 195-208
33. Wang G.H., and Seeger C. The reverse transcriptase of hepatitis B virus acts as a protein primer for viral DNA synthesis. Cell 71 (1992) 663-670
34. Tavis J.E., Perri S., and Ganem D. Hepadnavirus reverse transcription initiates within the stem-loop of the RNA packaging signal and employs a novel strand transfer. J. Virol. 68 (1994) 3536-3543
35. Wang G.H., Zoulim F., Leber E.H., Kitson J., and Seeger C. Role of RNA in enzymatic activity of the reverse transcriptase of hepatitis B viruses. J. Virol. 68 (1994) 8437-8442
36. Fallows D.A., and Goff S.P. Mutations in the epsilon sequences of human hepatitis B virus affect both RNA encapsidation and reverse transcription. J. Virol. 69 (1995) 3067-3073
37. Lott L., Beames B., Notvall L., and Lanford R.E. Interaction between hepatitis B virus core protein and reverse transcriptase. J. Virol. 74 (2000) 11479-11489