Evaluation of microbial RNA extractions from Streptococcus pneumoniae

Journal of Microbiological Methods

Volumn 68, Issue 2, 2007, Pages 342-348

  Li Korotky, Ha Sheng ^a,b   Kelly, Lori A ^a   Piltcher, Otavio ^a   Hebda, Patricia A ^a,b   Doyle, William J ^a,b  

^a CHILDREN S HOSPITAL OF PITTSBURGH   (United States)

^b UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

Method assessment; Microbial RNA extraction; Middle ear mucosa; Otitis media; Streptococcus pneumoniae

Indexed keywords

BACTERIAL RNA; MESSENGER RNA; PENICILLIN BINDING PROTEIN; PENICILLIN BINDING PROTEIN 2B; POLYINOSINIC ACID; REAGENT; RIBONUCLEASE; RNA 16S; TRIZOL; UNCLASSIFIED DRUG;

ACUTE OTITIS MEDIA; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL COLONIZATION; BACTERIAL VIRULENCE; COLONY FORMING UNIT; CONTROLLED STUDY; CYTOLYSIS; DRUG PROTEIN BINDING; INTERMETHOD COMPARISON; METHODOLOGY; MIDDLE EAR; MIDDLE EAR MUCOSA; NASOPHARYNX; NONHUMAN; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RIBONUCLEASE PROTECTION ASSAY; RNA ANALYSIS; RNA EXTRACTION; SAMPLING; SENSITIVITY AND SPECIFICITY; STREPTOCOCCUS INFECTION; STREPTOCOCCUS PNEUMONIAE;

ANIMALS; ENDOPEPTIDASE K; ENDOPEPTIDASES; GUANIDINES; HUMANS; OTITIS MEDIA; PHENOLS; PNEUMOCOCCAL INFECTIONS; POLY I; RATS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SODIUM DODECYL SULFATE; STREPTOCOCCUS PNEUMONIAE;

RATTUS; STREPTOCOCCUS PNEUMONIAE;

EID: 33846449463     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2006.07.014     Document Type: Article

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