Preparation of polyclonal antibody specific for AtPLC4, an Arabidopsis phosphatidylinositol-specific phospholipase C in rabbits

Protein Expression and Purification

Volumn 52, Issue 2, 2007, Pages 306-312

  Cao, Zhixiang ^a   Zhang, Jiewei ^a   Li, Yuan ^a   Xu, Xiaojing ^a   Liu, Guoqin ^a   Bhattacharrya, Madan K ^b   Yang, Hailian ^a   Ren, Dongtao ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b IOWA STATE UNIVERSITY   (United States)

Author keywords

Arabidopsis thaliana; AtPLC4; Phosphatidylinositol specific phospholipase C; Polyclonal antibody

Indexed keywords

PHOSPHATIDYLINOSITOL DIACYLGLYCEROL LYASE;

AMINO ACID SEQUENCE; ANIMAL; ANTIBODY PRODUCTION; ANTIBODY SPECIFICITY; ARABIDOPSIS; ARTICLE; CELL MEMBRANE; CYTOSOL; ENZYMOLOGY; IMMUNOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; RABBIT; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODY FORMATION; ANTIBODY SPECIFICITY; ARABIDOPSIS; CELL MEMBRANE; CONSERVED SEQUENCE; CYTOSOL; MOLECULAR SEQUENCE DATA; PHOSPHATIDYLINOSITOL DIACYLGLYCEROL-LYASE; RABBITS; SEQUENCE HOMOLOGY, AMINO ACID;

ARABIDOPSIS; ARABIDOPSIS THALIANA; ESCHERICHIA COLI; EUKARYOTA; ORYCTOLAGUS CUNICULUS;

EID: 33846422703     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.10.007     Document Type: Article

References (38)

1. Berridge M.J. Inositol trisphosphate and calcium signaling. Nature 361 (1993) 315-325
2. Rebecchi M.J., and Pentyala S.N. Structure, function, and control of phosphoinositide-specific phospholipase C. Phys. Rev. 80 (2000) 1291-1335
3. Lopez I., Mak E.C., Ding S., Hamm H.E., and Lomasney J.W. A novel bifunctional phospholipase C that is regulated by Galpha 12 and stimulates the Ras/mitogen-activated protein kinase pathway. J. Biol. Chem. 276 (2001) 2758-2765
4. Song C., Hu C.-D., Masago M., Kariya K.I., Yamawaki-Kataoka Y., Shibatohge M., Wu D., Satoh T., and Kataoka T. Regulation of a novel human phospholipase C, PLCε, through membrane targeting by Ras. J. Biol. Chem. 276 (2001) 2752-2757
5. 2+ oscillations in eggs and embryo development. Development 129 (2002) 3533-3544
6. Essen L.O., Perisc O., Cheung M., Kata M., and Williams R.L. Crystal structure of mammalian phosphoinositide-specific phospholipase Cδ. Nature 380 (1996) 595-602
7. Paterson H.F., Savopoulos J.W., Perisc O., Cheung R., Ellis M.V., Williams R.L., and Katan M. Phospholipase C delta 1 requires a pleckstrin homology domain for interaction with the plasma membrane. Biochem. J. 312 (1995) 661-666
8. Nakashima S., Banno Y., Watanabe T., Nakamura Y., Mizutani T., Sakai H., Zhao Y., Sugimoto Y., and Nozawa Y. Deletion and site-directed mutagenesis of EF-hand domain of phospolipase C-delta 1: effects on its activity. Biochem. Biophys. Res. Commun. 211 (1995) 364-369
9. Mueller-Roeber B., and Pical C. Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipidase C. Plant Physiol. 130 (2002) 22-46
10. Chapman K.D. Phospholipase activity during plant growth and development and in response to environmental stress. Trends Plant Sci. 11 (1998) 419-426
11. Ettlinger C., and Lehle L. Auxin induces rapid changes in phosphatidylinositol metabolites. Nature 331 (1988) 176-178
12. Staxén I., Pical C., Montgomery L.T., Gray J.E., Heterington A.M., and McAinsh M.R. Abscisic acid induces oscillations in guard cell cytosolic free calcium that involve phosphoinositide-specific phospholipase C. Proc. Natl. Acad. Sci. USA 96 (1999) 1779-1784
13. Hunt L., Mills L.N., Pical C., Leckie C.P., Aitken F.L., Kopka J., Müller-Röber B., McAinsh M.R., Heterington A.M., and Gray J.E. Phospholipase C is required for the control of stomata aperture by ABA. Plant J. 34 (2003) 47-55
14. Sanchez J.P., and Chua N.H. Arabidopsis PLC1 is required for secondary responses to abscisic acid signals. Plant Cell 13 (2001) 1143-1154
15. Morse M.J., Crain R.C., and Satter R.L. Light-stimulated inositol phospholipid turnover in Samanea saman leaves pulvini. Proc. Natl. Acad. Sci. USA 84 (1987) 7075-7078
16. Perera I.Y., Heilmann I., and Boss W.F. Transient and sustained increases in inositol 1,4,5-trisphosphate precede the differential growth response in gravistimulated maize pulvini. Proc Natl. Acad. Sci. USA 96 (1999) 5838-5843
17. Kurosaki F., Tsurusawa Y., and Nishi A. Breakdown of phosphatidylinositol during the elicitation of phytoalexin production in cultured carrot cells. Plant Physiol. 85 (1987) 601-604
18. Franklin-Tong V.E., Drøbak B.K., Allan A.C., Watkins P.A.C., and Trewavas A.J. Growth of pollen tubes of Papaver rhoeas is regulated by a slow-moving calcium wave propagated by inositol 1,4,5-triphosphate. Plant Cell 8 (1996) 1305-1321
19. Bristol A., Hall S.M., Kriz R.W., Stahl M.L., Fan Y.S., Byers M.G., Eddy R.L., Shows T.B., Campisi L., Yang Y., Yi Y., Heilig E., Herman B., Cassista A.J., Allen D.W., Xiang H., and Jack T. Generation of enhancer trap lines in Arabidopsis and characterization of expression patterns in the inflorescence. Plant J. 17 (1999) 699-707
20. Katan M., Kriz R.W., Totty N., Philp R., Meldrum E., Aldape R.A., Knopf J., and Parker P.J. Primary structure of PLC-154. Cold Spring Harb. Symp. Quant. Biol. 53 (1988) 921-926
21. Stahl J.H., Ferenz C.R., Kelleher K.L., Kriz R.W., and Knopf J.L. Sequence similarity of phospholipase C with the non-catalytic region of src. Nature 332 (1988) 269-272
22. Suh P.G., Ryu S.H., Moon K.H., Suh H.W., and Rhee S.G. Cloning and sequence of multiple forms of phospholipase C. Cell 54 (1988) 161-169
23. Hirayama T., Ohto C., Mizoguchi T., and Shinozaki K. A gene encoding a phosphatidylinositol-specific phospholipase C is induced by dehydration and salt stress in Arabidpsis thaliana. Proc. Natl. Acad. Sci. USA 92 (1995) 3903-3907
24. Shi J., Gonzales R.A., and Bhattacharyya M.K. Characterization of a plasma membrane-associated phosphoinsitide-specific phospholipase C from soybean. Plant J. 8 (1995) 381-390
25. Kopka J., Pical C., Gray J.E., and Müller-Röber B. Molecular and enzymatic characterization of three phosphoinositide-specific phospholipase C isoforms from potato. Plant Physiol. 116 (1998) 239-250
26. Pical C., Kopka J., Müller-Röber J., Hetheringto A.M., and Gray J.E. Isolation of 2 cDNA clones for phosphoinositide-specific phospholipase C from epidermal peels (Accession No. X95877) and guard cells (Accession No. Y11931) of Nicotiana rustica (PGR 97-086). Plant Physiol. 114 (1997) 747
27. Xu X.J., Cao Z.X., Liu G.Q., Bhattacharyya M.K., and Ren D.T. Cloning and expression of AtPLC6, a gene encoding a phosphatidylinositol-specific phospholipase C in Arabidopsis thaliana. Chin. Sci. Bull. 49 (2004) 567-573
28. Yamamoto Y.T., Conkling M.A., Sussex I.M., and Irish V.F. An Arabidopsis cDNA related to animal phosphoinositide-specific phospholipase C genes. Plant Physiol. 107 (1995) 1029-1030
29. Hirayama T., Misukawa N., Shibata D., and Shinozaki K. AtPLC2, a gene encoding phosphoinositide-specific phospholipase C, is constitutively expressed in vegetative and floral tissues in Arabidopsis thaliana. Plant Mol. Biol. 34 (1997) 175-180
30. Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., and Gray J.E. Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms. New Phytol. 162 (2004) 634-654
31. Otterhag L., Sommarin M., and Pical C. N-terminal EF-hand-like domain is required for phosphoinositide-specific phospholipase C activity in Arabidopsis thaliana. FEBS Lett. 497 (2001) 165-170
32. Dager D.A., and Burgess R.R. Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removed of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA is topoisomerase, and other enzymes. Anal. Biochem. 109 (1980) 760-786
33. Olmsted J.B. Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples. J. Biol. Chem. 256 (1981) 11955-11957
34. Larsson C., Widell S., and Kjellbom P. Preparation of high-purity plasma membranes. Methods Enzymol. 148 (1987) 558-568
35. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
36. Towbin H., Staehelin T., and Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets:procedure and some applications. Proc. Nat. Acad. Sci. USA 76 (1979) 4350-4354
37. Venkataraman G., Goswami M., Tuteja N., Reddy M.K., and Sopory S.K. Isolation and characterization of a phospholipase C delta isoform from pea that is regulated by light in a tissue specific manner. Mol. Genet. Gen. 270 (2004) 378-386
38. 2+ oscillations and egg activation in mammals is a novel phospholipase C: PLCζ. Reproduction 127 (2004) 431-439