Quantification of low-expressed mRNA using 5′ LNA-containing real-time PCR primers

Biochemical and Biophysical Research Communications

Volumn 354, Issue 1, 2007, Pages 246-252

  Malgoyre, A ^a   Banzet, S ^a   Mouret, C ^a   Bigard, A X ^a   Peinnequin, A ^a  

^a CENTRE DE RECHERCHES   (France)

Author keywords

Hybridization; Interleukin 6 (IL6); LNA primer design; Locked nucleic acid (LNA); Low expressed mRNA; Polymerase chain reaction (PCR)

Indexed keywords

GENOMIC DNA; LOCKED NUCLEIC ACID; MESSENGER RNA; PRIMER DNA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DNA FLANKING REGION; DNA HYBRIDIZATION; DNA TEMPLATE; DNA TRANSCRIPTION; HUMAN; NONHUMAN; NUCLEIC ACID AMPLIFICATION; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RAT; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS;

DNA PRIMERS; MICROCHEMISTRY; OLIGONUCLEOTIDES; OLIGONUCLEOTIDES, ANTISENSE; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY;

EID: 33846322849     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.12.194     Document Type: Article

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