Modifying the β,γ leaving-group bridging oxygen alters nucleotide incorporation efficiency, fidelity, and the catalytic mechanism of dna polymerase β

Biochemistry

Volumn 46, Issue 2, 2007, Pages 461-471

  Sucato, Christopher A ^a   Upton, Thomas G ^a   Kashemirov, Boris A ^a   Batra, Vinod K ^d   Martínek, Václav ^b,c   Xiang, Yun ^a   Beard, William A ^d   Pedersen, Lars C ^d   Wilson, Samuel H ^d   McKenna, Charles E ^a   Florián, Jan ^b,c   Warshel, Arieh ^a   Goodman, Myron F ^a  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^b LOYOLA UNIVERSITY CHICAGO   (United States)

^c CHARLES UNIVERSITY   (Czech Republic)

^d NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CONFORMATIONS; DNA; ENZYMES; FREE ENERGY; OXYGEN; PHOSPHATES;

BOND BREAKING; DNA POLYMERASE; NUCLEOTIDE BASES; NUCLEOTIDYL TRANSFER; PRIMERS; PYROPHOSPHATES;

CATALYSIS;

CHLORINE DERIVATIVE; DNA DIRECTED DNA POLYMERASE BETA; FLUORINE DERIVATIVE; NUCLEOTIDE; OXYGEN; PYROPHOSPHATE;

ARTICLE; BASE MISPAIRING; BETA CHAIN; CATALYSIS; CHEMICAL REACTION KINETICS; CONFORMATIONAL TRANSITION; DNA SYNTHESIS; ENERGY TRANSFER; ENZYME CONFORMATION; ENZYME MODIFICATION; ENZYME SPECIFICITY; HUMAN; NONHUMAN; PKA; PRIORITY JOURNAL; STEREOCHEMISTRY;

BASE SEQUENCE; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; DEOXYGUANINE NUCLEOTIDES; DNA; DNA POLYMERASE BETA; HUMANS; KINETICS; MODELS, MOLECULAR; NUCLEIC ACID CONFORMATION; NUCLEOTIDES; OXYGEN; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; THERMODYNAMICS;

EID: 33846272360     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi061517b     Document Type: Article

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