An ultraviolet photoacoustic spectroscopy study of the interaction between Lys49-phospholipase A2 and amphiphilic molecules

Biochemical and Biophysical Research Communications

Volumn 353, Issue 4, 2007, Pages 889-894

  Bugs, Milton Roque ^a,b   Bortoleto Bugs, Raquel Kely ^a,b   Cornélio, Marinônio Lopes ^c   Ward, Richard John ^d  

^a PETROBRAS   (Brazil)

^b CenPRA Centro de Pesquisas Renato Archer   (Brazil)

^c SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

^d UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Bothropstoxin I; Difference spectroscopy; Fluorescence; Photoacoustic spectroscopy

Indexed keywords

AMPHOPHILE; AROMATIC COMPOUND; BOTHROPSTOXIN I; DODECYL SULFATE SODIUM; HOMODIMER; MEMBRANE PROTEIN; PHENYLALANINE; PHOSPHOLIPASE A2; SNAKE VENOM; TRYPTOPHAN; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; CONCENTRATION (PARAMETERS); FLUORESCENCE ANALYSIS; FLUORESCENCE SPECTROSCOPY; INTRINSIC TRYPTOPHAN FLUORESCENCE EMISSION SPECTROSCOPY; LIGHT SCATTERING; MICROENVIRONMENT; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; NONHUMAN; PHOTOACOUSTIC SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN STRUCTURE; SIGNAL PROCESSING; SPECTRAL SENSITIVITY; ULTRAVIOLET RADIATION;

ANIMALS; BOTHROPS; CROTALID VENOMS; DIMERIZATION; LYSINE; MODELS, MOLECULAR; PHOSPHOLIPASES A; PROTEIN CONFORMATION; SODIUM DODECYL SULFATE; SPECTROPHOTOMETRY, ULTRAVIOLET; SURFACE-ACTIVE AGENTS;

BOTHROPS JARARACUSSU;

EID: 33846179660     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.12.085     Document Type: Article

References (24)

1. Rosencwaig A. Photoacoustics and Photoacoustic Spectroscopy (1980), John Wiley & Sons, New York USA
2. Bugs M.R., and Cornélio M.L. Analysis of the ethidium bromide bound to DNA by photoacoustic and FTIR spectroscopy. Photochem. Photobiol. 74 (2001) 512-520
3. Bugs M.R., and Cornélio M.L. A new biophysics approach using photoacoustic spectroscopy to study the DNA-ethidium bromide interaction. Eur. Biophys. J. 31 (2002) 232-240
4. Gutierrez J.M., and Lomonte B. Phospholipase A2 myotoxins from Bothrops snake venoms. Toxicon 33 (1995) 1405-1424
5. Arni R.K., and Ward R.J. Phospholipase A2-a structural review. Toxicon 34 (1996) 827-841
6. Homsi-Brandeburgo M.I., Queiroz L.S., Santo-Neto H., Rodrigues-Simioni L., and Giglio J.R. Fractionation of Bothrops jararacussu snake venom: partial chemical characterization and biological activity of bothropstoxin. Toxicon 26 (1988) 615-627
7. Ward R.J., Chioato L., de Oliveira A.H., Ruller R., and Sa J.M. Active-site mutagenesis of a Lys49-phospholipase A2: biological and membrane-disrupting activities in the absence of catalysis. Biochem. J. 362 (2002) 89-96
8. Fletcher J.E., and Jiang M.S. LYS49 phospholipase A2 myotoxins lyse cell cultures by two distinct mechanisms. Toxicon 36 (1998) 1549-1555
9. Diaz C., Gutierrez J.M., Lomonte B., and Gene J.A. The effect of myotoxins isolated from Bothrops snake venoms on multilamellar liposomes: relationship to phospholipase A2, anticoagulant and myotoxic activities. Biochim. Biophys. Acta 1070 (1991) 455-460
10. Rufini S., Cesaroni P., Desideri A., Farias R., Gubensek F., Gutierrez J.M., Luly P., Massoud R., Morero R., and Pedersen J.Z. Calcium ion independent membrane leakage induced by phospholipase-like myotoxins. Biochemistry 31 (1992) 12424-12430
11. Pedersen J.Z., de Arcuri B.F., Morero R.D., and Rufini S. Phospholipase-like myotoxins induce rapid membrane leakage of non-hydrolyzable ether-lipid liposomes. Biochim. Biophys. Acta 1190 (1994) 177-180
12. da Silva Giotto M.T., Garratt R.C., Oliva G., Mascarenhas Y.P., Giglio J.R., Cintra A.C., de Azevedo Jr. W.F., Arni R.K., and Ward R.J. Crystallographic and spectroscopic characterization of a molecular hinge: conformational changes in bothropstoxin I, a dimeric Lys49-phospholipase A2 homologue. Proteins 30 (1998) 442-454
13. 2+-independent membrane damaging activity. Biochemistry 40 (2001) 6912-6920
14. 2+-independent membrane-damaging activity in Lys49-phospholipase A2 promoted by amphiphilic molecules. Biochem. Biophys. Res. Commun. 322 (2004) 364-372
15. Bortoleto-Bugs R.K., Bugs M.R., Neto A.A., and Ward R.J. A micelle nucleation model for the interaction of dodecyl sulphate with Lys49-phospholipases A(2). Biophys. Chem. 125 (2007) 113-120
16. Perkampus H.-H. Recent Developments in UV-VIS Spectroscopy. In: Perkampus H.H. (Ed). UV-VIS Spectroscopy and Its Applications (1992), Springer-Verlag, Berlin Heidelberg 101-120
17. de Oliveira A.H., Giglio J.R., Andriao-Escarso S.H., and Ward R.J. The effect of resonance energy homotransfer on the intrinsic tryptophan fluorescence emission of the bothropstoxin-I dimer. Biochem. Biophys. Res. Commun. 284 (2001) 1011-1015
18. Ruller R., Ferreira T.L., de Oliveira A.H., and Ward R.J. Chemical denaturation of a homodimeric lysine-49 phospholipase A2: a stable dimer interface and a native monomeric intermediate. Arch. Biochem. Biophys. 411 (2003) 112-120
19. Ruller R., Aparecida-Aragão E., Chioato L., Ferreira-Lopes T., de Oliveira A.H.C., Sà J.M., and Ward R.J. A predominant role for hydrogen bonding in the stability of the homodimer of bothropstoxin-I, a lysine 49-phospholipase A2. Biochimie 87 (2005) 993-1003
20. T.E. Creighton, In: Proteins structures and molecular properties. W.H. Freman and Company, New York, 1993, pp. 14-17.
21. Valeur B., and Weber G. Resolution of the fluorescence spectrum of indole into the 1La and 1Lb excitation bands. Photochem. Photobiol. 25 (1977) 441-444
22. Ambrosio A.L., Nonato M.C., Selistre-de-Araujo H.S., Arni R., Ward R.J., Ownby C.L., de Souza D.H., and Garratt R.C. A molecular mechanism for Lys49-phospholipase A2 activity based on ligand-induced conformational change. J. Biol. Chem. 280 (2005) 7326-7335
23. Prabhu N., and Sharp K. Protein-solvent interactions. Chem. Rev. 106 (2006) 1616-1623
24. Prabhu N.V., and Sharp K.A. Heat capacity in proteins. Annu. Rev. Phys. Chem. 56 (2005) 521-548