Baculovirus-mediated gene silencing in insect cells using intracellularly produced long double-stranded RNA

Journal of Biotechnology

Volumn 128, Issue 2, 2007, Pages 226-236

  Huang, Yi ^a,c   Deng, Fei ^a   Hu, Zhihong ^a   Vlak, Just M ^b   Wang, Hanzhong ^a  

^a WUHAN INSTITUTE OF VIROLOGY   (China)

^b WAGENINGEN UNIVERSITY   (Netherlands)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Baculovirus; DNA vectors; Enhanced green fluorescent protein (eGFP); Gene expression; Long hairpin dsRNA; RNAi

Indexed keywords

BIOSYNTHESIS; DNA SEQUENCES; ENZYME INHIBITION; PLANTS (BOTANY); PROTEINS; RNA;

BACULOVIRUS; DNA VECTORS; ENHANCED GREEN FLUORESCENT PROTEIN (EGFP); GENE EXPRESSION; LONG HAIRPIN DSRNA;

GENES;

BACULOVIRUS VECTOR; DOUBLE STRANDED RNA; ENHANCED GREEN FLUORESCENT PROTEIN;

ANIMAL CELL; ARTICLE; BACULOVIRUS; BACULOVIRUS EXPRESSION SYSTEM; CONTROLLED STUDY; DNA TEMPLATE; DNA VECTOR; EXPRESSION VECTOR; GENE CONSTRUCT; GENE EXPRESSION REGULATION; GENE SILENCING; GENETIC TRANSFECTION; INSECT CELL; NONHUMAN; PLASMID; PRIORITY JOURNAL; RNA INTERFERENCE; RNA SYNTHESIS;

ANIMALS; BACULOVIRIDAE; CELL LINE; GENE EXPRESSION REGULATION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; PLASMIDS; RNA INTERFERENCE; RNA, DOUBLE-STRANDED; RNA, SMALL INTERFERING; SPODOPTERA; TRANSFECTION;

HEXAPODA; INSECTA; NEMATODA;

EID: 33846062087     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.09.016     Document Type: Article

References (28)

1. Agrawal N., Malhotra P., and Bhatnagar R.K. siRNA-directed silencing of transgene expressed in cultured insect cells. Bioch. Biophys. Res. Commun. 320 (2004) 428-434
2. Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., and Possee R.D. The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202 (1994) 586-605
3. Bernstein E., Caudy A.A., Hammond S.M., and Hannon G.J. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 18 (2001) 363-366
4. Chuang C.F., and Meyerowitz E.M. Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana. Proc. Natl. Acad. Sci. U.S.A. 97 (2000) 4985-4990
5. Coburn G.A., and Cullen B.R. Potent and specific inhibition of human immunodeficiency virus type 1 replication by RNA interference. J. Virol. 76 (2002) 9225-9231
6. Elbashir S.M., Lendeckel W., and Tuschl T. RNA interference is mediated by 21- and 22- nucleotide RNAs. Genes Dev. 15 (2001) 188-200
7. Fire A., Xu S., Montgomery M.K., Kostas S.A., Driver S.E., and Mello C.C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391 (1998) 806-811
8. Flores-Jasso C.F., Valdes V.J., Sampieri A., Valadez-Graham V., Recillas-Targa F., and Vaca L. Silencing structural and nonstructural genes in baculovirus by RNA interference. Virus Res. 102 (2004) 75-84
9. Hajós J.P., Vermunt A.M.W., Zuidema D., Kulcsar P., Varjas L., de Kort C.A.D., and Vlak J.M. Dissecting insect development: baculovirus-mediated gene silencing. Insect Mol. Biol. 8 (1999) 539-544
10. Hammond S.M., Bernstein E., Beach D., and Hannon G.J. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 404 (2000) 293-296
11. Kennerdell J.R., and Carthew R.W. Heritable gene silencing in Drosophila using double-stranded RNA. Nat. Biotechnol. 18 (2000) 896-898
12. Knight S.W., and Bass B.L. A role for the RNase III enzyme DCR-1 in RNA interference and germ line development in Caenorhabditis elegans. Science 293 (2001) 2269-2271
13. Luckow V.A., and Summers M.D. Signals important for high-level expression of foreign genes in Autographa californica nuclear polyhedrosis virus expression vectors. Virology 167 (1988) 56-71
14. Maeda I., Kohara Y., Yamamoto M., and Sugimoto A. Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi. Curr. Biol. 11 (2001) 171-176
15. Means J.C., Muro I., and Clem R.J. Silencing of the baculovirus Op-iap3 gene by RNA interference reveals that it is required for prevention of apoptosis during Orgyia pseudotsugata M nucleopolyhedrovirus infection of Ld652Y cells. J. Virol. 77 (2003) 4481-4488
16. Misquitta L., and Paterson B.M. Targeted disruption of gene function in Drosophila by RNA interference (RNAi): a role for nautilus in embryonic somatic muscle formation. Proc. Natl. Acad. Sci. U.S.A. 96 (1999) 1451-1456
17. Nagy P., Arndt-Jovin J., and Jovin T.M. Small interfering RNAs suppress the expression of endogenous and GFP-fused epidermal growth factor receptor (erbB1) and induce apoptosis in erbB1-overexpressing cells. Exp. Cell Res. 285 (2003) 39-49
18. Roelvink P.W., van Meer M.M.M., De Kort C.A.D., Possee R.D., Hammock B.D., and Vlak J.M. Dissimilar expression of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus polyhedrin and p10 genes. J. Gen. Virol. 73 (1992) 1481-1489
19. Sharp P.A. RNAi and double-strand RNA. Genes Dev. 13 (1999) 139-141
20. Sui G., Scoohoo C., Affar E.B., Gay F., Shi Y., Forrester W.C., and Shi Y. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 99 (2002) 5515-5520
21. Tavernarakis T., Wang S.L., Dorovkov M., Ryazaony A., and Driscoll M. Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes. Nat. Genet. 24 (2000) 180-183
22. Theilmann, D.A., Blissard, G.W., Bonning, B., Jehle, J.A., O'Reily, D.R., Rohrmann, G.F., Thiem, S., Vlak, J.M., 2005. Baculoviridae: In: Fauquet, C.M., Mayo, M.A., Maniloff, J., Desselberger, U., Ball, L.A. (Eds.) VIIIth Report of the International Committee on Taxonomy of Viruses. Elsevier, pp. 177-185.
23. Thijn R.B., Rene B., and Reuven A. A system for stable expression of short interfering RNAs in mammalian cells. Science 296 (2002) 550-553
24. Tiscomia G., Singer O., Ikawa M., and Verma I.M. A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA. Proc. Natl. Acad. Sci. U.S.A. 100 (2002) 1844-1848
25. Tuschl T., Zamore P.D., Lehmann R., Bartel D.P., and Sharp P.A. Targeted mRNA degradation by double-stranded RNA in vitro. Gene Dev. 13 (1999) 3191-3197
26. Valdes V.J., Sampieri A., Sepulveda J., and Vaca L. Using double-stranded RNA to prevent in vitro and in vivo viral infections by recombinant baculovirus. J. Biol. Chem. 278 (2003) 19317-19324
27. Westenberg, M., Heinhuis, B., Zuidema, D., Vlak, J.M., 2005. siRNA injection induces sequence-independent protection in Penaeus monodon against white spot syndrome virus. Virus Res., in press.
28. Zamore P.D., Tuschl T., Sharp P.A., and Bartel D.P. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21-23 nucleotide intervals. Cell 101 (2000) 25-33