An intact homeobox domain is required for complete nuclear localization of human Nanog

Biochemical and Biophysical Research Communications

Volumn 353, Issue 3, 2007, Pages 770-775

  Do, Hyun Jin ^a   Lim, Hye Young ^b   Kim, Jin Hoi ^c   Song, Hyuk ^d   Chung, Hyung Min ^b   Kim, Jae Hwan ^b  

^a Chabiotech Co Ltd   (South Korea)

^b CHA University   (South Korea)

^c Konkuk University   (South Korea)

^d Konkuk University   (South Korea)

Author keywords

Homeobox domain; Human Nanog; Nuclear localization signal

Indexed keywords

HOMEODOMAIN PROTEIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR NANOG; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; NONHUMAN; NUCLEAR IMPORT; NUCLEAR LOCALIZATION SIGNAL; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN DOMAIN; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; CELL NUCLEUS; DNA-BINDING PROTEINS; GENES, HOMEOBOX; HOMEODOMAIN PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEAR LOCALIZATION SIGNALS; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS;

EID: 33846025381     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.12.100     Document Type: Article

References (18)

1. Kuroda T., Tada M., Kubota H., Kimura H., Hatano S.Y., Suemori H., Nakatsuji N., and Tada T. Octamer and Sox elements are required for transcriptional cis regulation of Nanog gene expression. Mol. Cell. Biol. 25 (2005) 2475-2485
2. McKinnon C.M., and Docherty K. Pancreatic duodenal homeobox-1, PDX-1, a major regulator of beta cell identity and function. Diabetologia 44 (2001) 1203-1214
3. Chi N., and Epstein J.A. Getting your Pax straight: Pax proteins in development and disease. Trends Genet. 18 (2002) 41-47
4. Mitsui K., Tokuzawa Y., Itoh H., Segawa K., Murakami M., Takahashi K., Maruyama M., Maeda M., and Yamanaka S. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 113 (2003) 631-642
5. Chambers I., Colby D., Robertson M., Nichols J., Lee S., Tweedie S., and Smith A. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 113 (2003) 643-655
6. Rodda D.J., Chew J.L., Lim L.H., Loh Y.H., Wang B., Ng H.H., and Robson P. Transcriptional regulation of nanog by OCT4 and SOX2. J. Biol. Chem. 280 (2005) 24731-24737
7. Gorlich D., and Kutay U. Transport between the cell nucleus and the cytoplasm. Ann. Rev. Cell Dev. Biol. 15 (1999) 607-660
8. Schwoebel E.D., and Moore M.S. The control of gene expression by regulated nuclear transport. Essays Biochem. 36 (2000) 105-113
9. Jans D.A., Xiao C.Y., and H Lam M. Nuclear targeting signal recognition: a key control point in nuclear transport?. BioEssays 22 (2000) 532-544
10. Oh J.H., Do H.J., Yang H.M., Moon S.Y., Cha K.Y., Chung H.M., and Kim J.H. Identification of a putative transactivation domain in human Nanog. Exp. Mol. Med. 37 (2005) 250-254
11. Do H.J., Song H., Yang H.M., Kim D.K., Kim N.H., Kim J.H., Cha K.Y., Chung H.M., and Kim J.H. Identification of multiple nuclear localization signals in murine Elf3, an ETS transcription factor. FEBS Lett. 580 (2006) 1865-1871
12. Sock E., Enderich J., Rosenfeld M.G., and Wegner M. Identification of the nuclear localization signal of the POU domain protein Tst-1/Oct6. J. Biol. Chem. 271 (1996) 17512-17518
13. Ghaffari M., Zeng X., Whitsett J.A., and Yan C. Nuclear localization domain of thyroid transcription factor-1 in respiratory epithelial cells. Biochem. J. 328 (1997) 757-761
14. Hessabi B., Ziegler P., Schmidt I., Hessabi C., and Walther R. The nuclear localization signal (NLS) of PDX-1 is part of the homeodomain and represents a novel type of NLS. Eur. J. Biochem. 263 (1999) 170-177
15. Moede T., Leibiger B., Pour H.G., Berggren P., and Leibiger I.B. Identification of a nuclear localization signal, RRMKWKK, in the homeodomain transcription factor PDX-1. FEBS Lett. 461 (1999) 229-234
16. Hessabi B., Schmidt I., and Walther R. The homeodomain of Nkx2.2 carries two cooperatively acting nuclear localization signals. Biochem. Biophys. Res. Commun. 270 (2000) 695-700
17. Christophe-Hobertus C., Duquesne V., Pichon B., Roger P.P., and Christophe D. Critical residues of the homeodomain involved in contacting DNA bases also specify the nuclear accumulation of thyroid transcription factor-1. Eur. J. Biochem. 265 (1999) 491-497
18. Damante G., Fabbro D., Pellizzari L., Civitareale D., Guazzi S., Polycarpou-Schwartz M., Cauci S., Quadrifoglio F., Formisano S., and Di Lauro R. Sequence-specific DNA recognition by the thyroid transcription factor-1 homeodomain. Nucleic Acids Res. 22 (1994) 3075-3083