The nuclear localization signal (NLS) of PDX-1 is part of the homeodomain and represents a novel type of NLS

European Journal of Biochemistry

Volumn 263, Issue 1, 1999, Pages 170-177

  Hessabi, Behnam ^a   Ziegler, Patrick ^a   Schmidt, Ines ^a   Hessabi, Claudia ^a   Walther, Reinhard ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

cell; Homeodomain proteins; Nuclear localization signal; Nuclear transport; Transcription factors

Indexed keywords

GREEN FLUORESCENT PROTEIN; HELIX LOOP HELIX PROTEIN; HOMEODOMAIN PROTEIN; PANCREAS POLYPEPTIDE; SOMATOSTATIN; TRANSACTIVATOR PROTEIN; TRANSCRIPTION FACTOR;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; B LYMPHOCYTE; CELL NUCLEUS; CELL STRAIN COS1; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DELETION MUTANT; DNA BINDING; ELECTROPHORETIC MOBILITY; FLUORESCENCE MICROSCOPY; GENE EXPRESSION REGULATION; GENE TRANSLOCATION; GENETIC TRANSFECTION; GLUCOSE HOMEOSTASIS; HUMAN; HUMAN CELL; IMMUNOBLOTTING; MOLECULAR CLONING; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; ORGANOGENESIS; PANCREAS; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; TRANSACTIVATION;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELL LINE; CELL NUCLEUS; CLONING, MOLECULAR; COS CELLS; DNA PRIMERS; GREEN FLUORESCENT PROTEINS; HOMEODOMAIN PROTEINS; ISLETS OF LANGERHANS; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEAR LOCALIZATION SIGNALS; RECOMBINANT FUSION PROTEINS; TRANS-ACTIVATORS; TRANSFECTION;

EID: 0033168391     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00481.x     Document Type: Article

References (48)

1. 1. Soares, M.B., Schön, E., Henderson, A., Karathanasis, S.K., Cate, R., Zeitlin, S., Chirgwin, J. & Efstratiadis, A. (1985) RNA-mediated gene duplication: the rat preproinsulin I gene is a functional retroposon. Mol. Cell. Biol. 5, 2090-2103.
2. 2. Laimins, L., Holmgren-König, M. & Khoury, G. (1985) Transcriptional 'silencer' element in rat repetitive sequences associated with the rat insulin 1 gene locus. Proc. Natl Acad. Sci. USA 83, 3151-3155.
3. 3. Fromont-Racine, M., Bucchini, D., Madsen, O., Desbois, P., Linde, S., Nielsen, J.H., Saulnier, C., Ripoche, M.A., Jami, J. & Pictet, R. (1990) Effect of 5′-flanking sequence deletions on expression of the human insulin gene in transgenic mice. Mol. Endocrinol. 4, 669-677.
4. 4. Walker, M.D., Edlund, T., Boulet, A.M. & Rutter, W.J. (1983) Cell-specific expression controlled by the 5′-flanking region of insulin and chymotrypsin genes. Nature 306, 557-561.
5. 5. Edlund, T., Walker, M.D., Barr, P.J. & Rutter, W.J. (1985) Cell-specific expression of the rat insulin gene: evidence for role of two distinct 5′ flanking elements. Science 230, 912-916.
6. 6. Karlsson, O., Edlund, T., Moss, J.B., Rutter, W.J. & Walker, M.D. (1987) A mutational analysis of the insulin gene transcription control region: expression in beta cells is dependent on two related sequences within the enhancer. Proc. Natl Acad. Sci. USA 84, 8819-8823.
7. 7. German, M.S. & Wang, J. (1994) The insulin gene contains multiple transcriptional elements that respond to glucose. Mol. Cell. Biol. 14, 4067-4075.
8. 8. Petersen, H.V., Serup, P., Leonard, J., Michelsen, B.K. & Madsen, O.D. (1994) Transcriptional regulation of the human insulin gene is dependent on the homeodomain protein STF1/IPF1 acting through the CT boxes. Proc. Natl Acad. Sci. USA 91, 10465-10469.
9. 9. Peers, B., Leonard, J., Sharma, S., Teitelman, G. & Montminy, M.R. (1994) Insulin expression in pancreatic islet cells relies on cooperative interactions between the helix loop helix factor E47 and the homeobox factor STF-1. Mol. Endocrinol. 8, 1798-1806.
10. 10. Murre, C., McCaw, P.S. & Baltimore, D. (1989) A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins. Cell 56, 777-783.
11. 11. Shieh, S.Y. & Tsai, M.J. (1991 ) Cell-specific and ubiquitous factors are responsible for the enhancer activity of the rat insulin II gene. Biol. Chem. 266, 16708-16714.
12. 12. German, M.S., Blanar, M.A., Nelson, C., Moss, L.G. & Rutter, W.J. (1991) Two related helix-loop-helix proteins participate in seperate cell-specific complexes that bind to the insulin enhancer. Mol. Endocrinol. 5, 292-299.
13. 13. Cordle, S.R., Whelan, J., Henderson, E., Masuoka, H., Weil, P.A. & Stein, R. (1991) Insulin gene expression in nonexpressing cells appears to be regulated by multiple distinct negative-acting control elements. Mol. Cell. Biol. 11, 2881-2886.
14. 14. Hu, J.S., Olson, E.N. & Kingston, R.E. (1992) HEB, a helix-loop-helix protein related to E2A and ITF2 that can modulate the DNA-binding ability of myogenic regulatory factors. Mol. Cell. Biol. 12, 1031-1042.
15. 15. Peyton, M., Moss, L.G. & Tsai, M.J. (1994) Two distinct class A helix-loop-helix transcription factors, E2A and BETA 1, form separate DNA binding complexes on the insulin gene. Biol. Chem. 269, 25936-25941.
16. 16. Naya, F.J., Stellrecht, C.M. & Tsai, M.J. (1995) Tissue-specific regulation of the insulin gene by a novel basic helix-loop-helix transcription factor. Genes Dev. 9, 1009-1019.
17. 17. Lee, J.E., Hollenberg, S.M., Snider, L., Turner, D.L., Lipnick, N. & Weintraub, H. (1995) Conversion of Xenopus ectoderm into neurons by NeuroD, a basic helix-loop-helix protein. Science 268, 836-844.
18. 18. Read, M.L., Clark, A.R. & Docherty, K. (1993) The helix-loop-helix transcription factor USF (upstream stimulating factor) binds to a regulatory sequence of the human insulin gene enhancer. Biochem. J. 295, 233-237.
19. 19. Gehring, W.J., Affolter, M. & Bürglin, T. (1994) Homeodomain proteins. Annu. Rev. Biochem. 63, 487-526.
20. 20. Ohlsson, H., Karlsson, K. & Edlund, T. (1993) IPF1, a homeodomain-containing transactivator of the insulin gene. EMBO J. 12, 4251-4259.
21. 21. Leonard, J., Peers, B., Johnson, T., Ferreri, K., Lee, S. & Montminy, M.R. (1993) Characterization of somatostatin transactivating factor-1, a novel homeobox factor that stimulates somatostatin expression in pancreatic islet cells. Mol. Endocrinol. 7, 1275-1283.
22. 22. Miller, C.P., McGehee, R.E. Jr & Habener, J.F. (1994) A new homeodomain transcription factor expressed in rat pancreatic islets and duodenum that transactivates the somatostatin gene. EMBO J. 13, 1145-1156.
23. 23. Ohlsson, H., Thor, S. & Edlund, T. (1991) Novel insulin promoter-and enhancer-binding proteins that discriminate between pancreatic alpha-and beta-cells. Mol. Endocrinol 5, 897-904.
24. 24. German, M.S., Wang, J., Chadwick, R.B. & Rutter, W.J. (1992) Synergistic activation of the insulin gene by a LIM-homeodomain protein and a basic helix-loop-helix protein: building a functional insulin minienhancer complex. Genes Dev. 6, 2165-2176.
25. 25. Karlsson, O., Thor, S., Norberg, T., Ohlsson, H. & Edlund, T. (1990) Insulin gene enhancer binding protein Isl-1 is a member of a novel class of proteins containing both a homeo-and a Cys-His domain. Nature 344, 879-882.
26. 26. Emens, L.A., Landers, D.W. & Moss, L.G. (1992) Hepatocyte nuclear factor 1 alpha is expressed in a hamster insulinoma line and transactivates the rat insulin I gene. Proc. Natl Acad. Sci. USA 89, 7300-7304.
27. 27. Wright, C.V.E., Schnegelsberg, P. & De Robertis, E.M. (1988) X1Hbox 8: a novel Xenopus homeo protein restricted to a narrow band of endoderm. Development 104, 787-794.
28. 28. Rudnick, A., Ling, T.Y., Odagiri, H., Rutter, W.J. & German, M.S. (1994) Pancreatic beta cells express a diverse set of homeobox genes. Proc. Natl Acad. Sci. USA 91, 12203-12207.
29. 29. Jonsson, J., Carlsson, L., Edlund, T. & Edlund, H. (1994) Insulin-promoter-factor 1 is required for pancreas development in mice. Nature 371, 606-609.
30. 30. Offield, M.F., Jetton, T.L., Labosky, P.A., Ray, M., Stein, R.W., Magnuson, M.A., Hogan, B.L. & Wright, C.V. (1996) PDX-1 is required for pancreatic outgrowth and differentiation of the rostral duodenum. Development 122, 983-995.
31. 31. Guz, Y., Montminy, M.R., Stein, R., Leonard, J., Gamer, L.W., Wright, C.V.E. & Teitelmann, G. (1995) Expression of murine STF-1, a putative insulin gene transcription factor, in beta cells of pancreas, duodenal epithelium and pancreatic exocrine and endocrine progenitors during ontogeny. Development 121, 11-18.
32. 32. Peers, B., Sharma, S., Johnson, T., Kamps, M. & Montminy, M. (1995) The pancreatic islet factor STF-1 binds cooperatively with Pbx to a regulatory element in the somatostatin promoter: importance of the FPWMK motif and the homeodomain. Mol. Cell. Biol. 15, 7091-7097.
33. 33. Lu, M., Miller, C.P. & Habener, J.E. (1996) Functional regions of the homeodomain protein IDX-1 required for transactivation of the rat somatostatin gene. Endocrinology 137, 2959-2967.
34. 34. Peshavaria, M., Henderson, E., Sharma, A., Wright, C. & Stein, R. (1997) Functional characterization of the transactivation properties of the PDX-1 homeodomain protein. Mol. Cell. Biol. 17, 3987-3996.
35. 35. Ausübel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (1992) Short Protocols in Molecular Biology, 2nd edn. John Wiley & Sons, New York.
36. 36. Leibiger, I.B., Schwarz, T., Leibiger, B. & Walther, R. (1995) Functional analysis of a newly identified TAAT-box of the rat insulin-II gene promoter. FEBS Lett. 362, 210-214.
37. 37. MacFarlane, W., Read, M.L., Gilligan, M., Bujalska, I. & Docherty, K. (1994) Glucose modulates the binding activity of the β cell transcription factor IUF-1 in a phosphorylation-dependent manner. Biochemical J. 303, 625-631.
38. 38. Macfarlane, W.M., Smith, S.B., James, R.F., Clifton, A.D., Doza, Y.N., Cohen, P. & Docherty, K. (1997) The p38/reactivating kinase mitogen-activated protein kinase cascade mediates the activation of the transcription factor insulin upstream factor 1 and insulin gene transcription by high glucose in pancreatic beta-cells. J. Biol. Chem. 272, 20936-20944.
39. 39. Dingwall, C. & Laskey, R.A. (1991) Nuclear targeting sequences - a consensus? Trends Biochem. Sci. 16, 478-481.
40. 40. Görlich, D. (1997) Nuclear protein import. Curr. Opin. Cell Biol. 9, 412-419.
41. 41. Vandromme, M., Cavadore, J.-C., Bonnieu, A., Froeschl, A., Lamb, N. & Fernandez, A. (1995) Two nuclear localization signals present in the basic-helix 1 domains of MyoD promote its active nuclear translocation and can function independently. Proc. Natl Acad. Sci. USA 92, 4646-4650.
42. 42. Schreiber, S., Molinete, M., Boeuf, H., de Murcia, G. & Menissier-de Murcia, J. (1992) The human poly (ADP-ribose) polymerase nuclear localization signal is a bipartite element functionally separate from DNA binding and catalytic activity. EMBO J. 11, 3263-3269.
43. 43. Meisel, L. & Lam, E, (1996) The conserved ELK-homeodomain of KNOTTED-1 contains two regions that signal nuclear localization. Plant. Mol. Biol. 30, 1-14.
44. 44. Hall, M.N., Craik, C. & Hiraoka, Y. (1990) Homeodomain of yeast repressor alpha 2 contains a nuclear localization signal. Proc. Natl Acad. Sci. USA 87, 6954-6958.
45. 45. Carriere, C., Plaza, S., Caboche, J., Dozier, C., Bailly, M., Martin, P. & Saule, S. (1995) Nuclear localization signals, DNA binding, and transactivation properties of quail Pax-6 (Pax-QNR) isoforms. Cell Growth Differ. 6, 1531-1540.
46. 46. Poleev, A., Okladnova, O., Musti, A.M., Schneider, S., Royer-Pokora, B. & Plachov, D. (1997) Determination of functional domains of the human transcription factor PAX8 responsible for its nuclear localization and transactivating potential. Eur. J. Biochem. 247, 860-869.
47. 47. Sock, E., Enderich, J., Rosenfeld, M.G. & Wegner, M. (1996) Identification of the nuclear localization signal of the POU domain protein Tst-1/Oct6. J. Biol. Chem. 271, 17512-17518.
48. 48. Robbins, J., Dilworth, S.M., Laskey, R.A. & Dingwall, C. (1991) Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence. Cell 64, 615-623.