Growth factor-independent 1B gene (GFI1B) is overexpressed in erythropoietic and megakaryocytic malignancies and increases their proliferation rate

British Journal of Haematology

Volumn 136, Issue 2, 2007, Pages 212-219

  Elmaagacli, Ahmet H ^a   Koldehoff, Michael ^a   Zakrzewski, Johannes L ^b   Steckel, Nina K ^a   Ottinger, Hellmut ^a   Beelen, Dietrich W ^a  

^a UNIVERSITY HOSPITAL ESSEN   (Germany)

^b Memorial Sloan Kettering   (United States)

Author keywords

Growth factor independent1B; Small interfering RNA

Indexed keywords

GROWTH FACTOR INDEPENDENT 1B PROTEIN; SMALL INTERFERING RNA; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ACUTE GRANULOCYTIC LEUKEMIA; ACUTE LYMPHOBLASTIC LEUKEMIA; ACUTE MEGAKARYOCYTIC LEUKEMIA; APLASTIC ANEMIA; APOPTOSIS; ARTICLE; CANCER PATIENT; CELL PROLIFERATION; CHRONIC MYELOID LEUKEMIA; CONTROLLED STUDY; ERYTHROLEUKEMIA; GENE; GENE OVEREXPRESSION; GENE SILENCING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LEUKEMIA CELL; MYELODYSPLASTIC SYNDROME; MYELOFIBROSIS; MYELOID METAPLASIA; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

ANEMIA, APLASTIC; ANTIGENS, CD34; APOPTOSIS; CASE-CONTROL STUDIES; CELL CYCLE; CELL LINE, TUMOR; GENE EXPRESSION; GENE EXPRESSION REGULATION, NEOPLASTIC; GENES, MYC; HUMANS; IMMUNOPHENOTYPING; LEUKEMIA; LEUKEMIA, ERYTHROBLASTIC, ACUTE; LEUKEMIA, MEGAKARYOCYTIC, ACUTE; PROTO-ONCOGENE PROTEINS; REPRESSOR PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHO GTP-BINDING PROTEINS; RNA INTERFERENCE; RNA, MESSENGER; RNA, SMALL INTERFERING; STATISTICS, NONPARAMETRIC; TRANSFECTION;

EID: 33845913519     PISSN: 00071048     EISSN: 13652141     Source Type: Journal    
DOI: 10.1111/j.1365-2141.2006.06407.x     Document Type: Article

References (24)

1. Duan, Z. & Horwitz, M. (2003) Targets of the transcriptional repressor oncoprotein Gfi-1. Proceedings of the National Academy of Sciences of the United States of America. , 100, 5932-5937.
2. Elbashir, S.M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K. & Tuschl, T. (2001) Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature, 411, 494-498.
3. Elmaagacli, A.H., Freist, A., Hahn, M., Opalka, B., Seeber, S., Schaefer, U.W. & Beelen, D.W. (2001) Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of bcr-abl fusion transcripts detected using a new real-time polymerase chain reaction method. British Journal of Haematology, 113, 1072-1075.
4. Elmaagacli, A.H., Koldehoff, M., Peceny, R., Klein-Hitpass, L., Ottinger, H., Beelen, D.W. & Opalka, B. (2005) WT1 and BCR-ABL specific small interfering RNA have additive effects in the induction of apoptosis in leukemic cells. Haematologica, 90, 326-334.
5. Garcon, L., Lacout, C., Svinartchouk, F., Le Couédic, J.-P., Villeval, J.-L., Vanchenker, W. & Dume nil, D. (2005) Gfi-1B plays a critical role in terminal differentiation of normal and transformed erythroid progenitor cells. Blood, 105, 1448-1455.
6. Gilks, C.B., Bear, S.E., Grimes, H.L. & Tsichlis, P.N. (1993) Progression of interleukin-2 (IL-2)-dependent rat T cell lymphoma lines to IL-2-independent growth following activation of a gene (Gfi-1) encoding a novel zinc finger protein. Molecular and Cell Biology, 13, 1759-1768.
7. Grimes, H.L., Gilks, C.B., Chan, T.O., Porter, S. & Tsichlis, P.N. (1996) The Gfi-1 proto-oncoprotein represses Bax expression and inhibits T-cell death. Proceedings of the National Academy of Sciences of the United States of America. , 93, 14569-14573.
8. Janssens, N., Janicot, M., Perera, T. & Bakker, A. (2004) Housekeeping genes as internal standards in cancer research. Molecular Diagnostics, 8, 107-113.
9. Karsunky, H., Mende, I., Schmidt, T. & Möröy, T. (2002a) High levels of the onco-protein GFI-1 accelerate T-cell proliferation and inhibit activation induced T-cell death in Jurkat T-cells. Oncogene, 21, 1571-1579.
10. Karsunky, H., Zeng, H., Schmidt, T., Zevnik, B., Kluge, R., Schmid, K.W., Dührsen, U. & Möröy, T. (2002b) Inflammatory reactions and severe neutropenia in mice lacking the transcriptional repressor GFI1. Nature Genetics, 30, 295-300.
11. Koldehoff, M., Steckel, N.K., Beelen, D.W. & Elmaagacli, A.H. (2006) Synthetics small interfering RNAs reduce bcr-abl gene expression in leukaemic cells of de novo Philadelphia (+) acute myeloid leukemia. Clinical Experimental Medicine, 6, 45-47.
12. Morgan, D.O. (1995) Principals of CDK regulation. Nature, 374, 131-134.
13. Osawa, M., Yamaguchi, T., Nakamura, Y., Kaneko, S., Ondera, M., Sawada, K.-I., Jegalian, A.W.H., Nakauchi, H. & Iwama, A. (2002) Erythroid expansion mediated by the Gfi-1B zinc finger protein: role in normal hematopoiesis. Blood, 100, 2769-2777.
14. Ottinger, H.D., Beelen, D.W., Scheulen, B., Schaefer, U.W. & Grosse-Wilde, H. (1996) Improved immune reconstitution after allotransplantation of peripheral blood stem cells instead of bone marrow. Blood, 88, 2775-2779.
15. Roberts, T. & Cowell, J.K. (1997) Cloning of the human gfi-1 gene and its mapping to chromosome region 1p22. Oncogene, 14, 1003-1005.
16. Rödel, B., Wagner, T., Zornig, M., Niessing, J. & Moroy, T. (1998) The human homologue (GFI1B) of the chicken GFI gene maps to chromosome 9q34.13-A locus frequently altered in hematopoietic diseases. Genomics, 54, 580-582.
17. Rödel, B., Tavassoli, K., Karsunky, H., Schmidt, T., Bachmann, M., Shaper, F., Heinrich, P., Shuai, K., Elsässer, H.P. & Möröy, T. (2000) The zinc finger protein Gfi-1 can enhance STAT3 signaling by interacting with the STAT3 inhibitor PIAS3. The EMBO Journal, 19, 5845-5855.
18. Saleque, S., Cameron, S. & Orkin, S.H. (2002) The zinc-finger protooncogene Gfi-1B is essential for the development of the erythroid and megakaryocytic lineages. Genes & Development, 16, 301-306.
19. Scherr, M., Battmer, K., Winkler, T., Heidenreich, O., Ganser, A. & Eder, M. (2003) Specific inhibition of BCR-ABL gene expression by small interfering RNA. Blood, 101, 1566-1569.
20. Schneider-Stock, R., Boltzke, C., Jäger, V., Epplen, J., Landt, O., Peters, B., Rys, J. & Roesner, A. (2003) Elevated telomerase activity, c-MYC-, and hTERT mRNA expression: association with tumour progression in malignant lipomatous tumours. Journal of Pathology, 199, 517-525.
21. Specht, K., Richter, T., Müller, U., Walch, A., Werner, M. & Höfler, H. (2001) Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue. American Journal of Pathology, 158, 419-429.
22. Tong, B., Grimes, H.L., Yang, T.-Y., Bear, S.E., Qin, Z., Du, K., El-Deiry, W.S. & Tsichlis, P.N. (1998) The Gfi-1B proto-oncoprotein represses p21WAF1 and inhibits myeloid cell differentiation. Molecular and Cell Biology, 18, 2462-2473.
23. Wilda, M., Fuchs, U., Wössmann, W. & Borkhardt, A. (2002) Killing of leukemic cells with a BCR/ABL fusion gene by RNA interference. Oncogene, 21, 5716-5724.
24. Wohlbold, L., Van Der Kuip, H., Miething, C., Vornlocher, H.P., Knappe, C., Duyster, J. & Aulitzky, W.E. (2003) Inhibition of BCR-ABL gene expression by small interfering RNA sensitizes for imatinib mesylate (ST571). Blood, 102, 2236-2239.