High-level heterologous gene expression in Ochrobactrum anthropi using an A-rich UP element

Applied Microbiology and Biotechnology

Volumn 73, Issue 5, 2007, Pages 1123-1127

  Seleem, Mohamed ^a   Ali, Mohammed ^b   Abd Al Azeem, M W ^c   Boyle, Stephen M ^a   Sriranganathan, Nammalwar ^a  

^a VIRGINIA MARYLAND REGIONAL COLLEGE OF VETERINARY MEDICINE   (United States)

^b Assiut University   (Egypt)

^c South Valley University   (Egypt)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; ENZYMES; GENES; PROTEINS; RNA;

GENE EXPRESSIONS; OCHROBACTRUM ANTHROPI; RNA POLYMERASE; TRANSCRIPTION EFFICIENCY;

BACTERIA;

ADENINE;

AMINO ACID; BACTERIUM; DNA; GENE EXPRESSION; PROTEIN; SOIL MICROORGANISM;

ARTICLE; DNA FLANKING REGION; GENE EXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; OCHROBACTRUM ANTHROPI; PROTEIN ANALYSIS; PROTEIN PURIFICATION;

BETA-GALACTOSIDASE; BLOTTING, WESTERN; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION; GENES, REPORTER; MOLECULAR BIOLOGY; MOLECULAR SEQUENCE DATA; OCHROBACTRUM ANTHROPI; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; REGULATORY ELEMENTS, TRANSCRIPTIONAL; SEQUENCE ANALYSIS, DNA;

OCHROBACTRUM ANTHROPI;

EID: 33845803726     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0555-7     Document Type: Article

References (20)

1. Aiyar SE, Gourse RL, Ross W (1998) Upstream A-tracts increase bacterial promoter activity through interactions with the RNA polymerase alpha subunit. Proc Natl Acad Sci USA 95:14652-14657
2. Amann E, Brosius J, Ptashne M (1983) Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25:167-178
3. Asano Y, Mori T, Hanamoto S, Kato Y, Nakazawa A (1989a) A new D-stereospecific amino acid amidase from Ochrobactrum anthropi. Biochem Biophys Res Commun 162:470-474
4. Asano Y, Nakazawa A, Kato Y, Kondo K (1989b) Properties of a novel D-stereospecific aminopeptidase from Ochrobactrum anthropi. J Biol Chem 264:14233-14239
5. Asano Y, Kato Y, Yamada A, Kondo K (1992) Structural similarity of D-aminopeptidase to carboxypeptidase DD and beta-lactamases. Biochemistry 31:2316-2328
6. Christenson JC, Pavia AT, Seskin K, Brockmeyer D, Korgenski EK, Jenkins E, Pierce J, Daly JA (1997) Meningitis due to Ochrobactrum anthropi: an emerging nosocomial pathogen. A report of 3 cases. Pediatr Neurosurg 27:218-221
7. Estrem ST, Gaal T, Ross W, Gourse RL (1998) Identification of an UP element consensus sequence for bacterial promoters. Proc Natl Acad Sci USA 95:9761-9766
8. Fanuel L, Thamm I, Kostanjevecki V, Samyn B, Joris B, Goffin C, Brannigan J, Van Beeumen J, Frere JM (1999) Two new aminopeptidases from Ochrobactrum anthropi active on D-alanyl-p-nitroanilide. Cell Mol Life Sci 55:812-818
9. Gard JK, Feng PC, Hutton WC (1997) Nuclear magnetic resonance timecourse studies of glyphosate metabolism by microbial soil isolates. Xenobiotica 27:633-644
10. Higgins CS, Avison MB, Jamieson L, Simm AM, Bennett PM, Walsh TR (2001) Characterization, cloning and sequence analysis of the inducible Ochrobactrum anthropi AmpC beta-lactamase. J Antimicrob Chemother 47:745-754
11. Laura D, De Socio G, Frassanito R, Rotilio D (1996) Effects of atrazine on Ochrobactrum anthropi membrane fatty acids. Appl Environ Microbiol 62:2644-2646
12. Lebuhn M, Achouak W, Schloter M, Berge O, Meier H, Barakat M, Hartmann A, Heulin T (2000) Taxonomic characterization of Ochrobactrum sp. isolates from soil samples and wheat roots, and description of Ochrobactrum tritici sp. nov. and Ochrobactrum grignonense sp. nov. Int J Syst Evol Microbiol 50(Pt 6):2207-2223
13. Lechner U, Baumbach R, Becker D, Kitunen V, Auling G, Salkinoja-Salonen M (1995) Degradation of 4-chloro-2-methylphenol by an activated sludge isolate and its taxonomic description. Biodegradation 6:83-92
14. Makrides SC (1996) Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol Rev 60:512-538
15. Ogawa J, Takeda S, Xie SX, Hatanaka H, Ashikari T, Amachi T, Shimizu S (2001) Purification, characterization, and gene cloning of purine nucleosidase from Ochrobactrum anthropi. Appl Environ Microbiol 67:1783-1787
16. Schafer A, Konrad R, Kuhnigk T, Kampfer P, Hertel H, Konig H (1996) Hemicellulose-degrading bacteria and yeasts from the termite gut. J Appl Bacteriol 80:471-478
17. Seleem MN, Vemulapalli R, Boyle SM, Schurig GG, Sriranganathan N (2004) Improved expression vector for Brucella species. Biotechniques 37:740-744
18. Sonke T, Ernste S, Tandler RF, Kaptein B, Peeters WP, Van Assema FB, Wubbolts MG, Schoemaker HE (2005) L-selective amidase with extremely broad substrate specificity from Ochrobactrum anthropi NCIMB 40321. Appl Environ Microbiol 71:7961-7973
19. Vemulapalli R, He Y, Boyle SM, Sriranganathan N, Schurig GG (2000) Brucella abortus strain RB51 as a vector for heterologous protein expression and induction of specific Th1 type immune responses. Infect Immun 68:3290-3296
20. Yu WL, Lin CW, Wang DY (1998) Clinical and microbiologic characteristics of Ochrobactrum anthropi bacteremia. J Formos Med Assoc 97:106-112