5-Azacytidine induces changes in electrophysiological properties of human mesenchymal stem cells

Cell Research

Volumn 16, Issue 12, 2006, Pages 949-960

  Balana, Bartosz ^a,b   Nicoletti, Cecilia ^a   Zahanich, Ihor ^a   Graf, Eva M ^a   Christ, Torsten ^a   Boxberger, Sabine ^a   Ravens, Ursula ^a  

^a DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^b SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

Author keywords

5 azacytidine; Cardiac differentiation; Human mesenchymal stem cells; Outward K+ currents

Indexed keywords

AZACITIDINE; CALCIUM CHANNEL; POTASSIUM CHANNEL;

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CYTOLOGY; DRUG EFFECT; ELECTROPHYSIOLOGY; HEART MUSCLE CELL; HUMAN; MESENCHYMAL STEM CELL; METABOLISM; PHYSIOLOGY;

AZACITIDINE; CALCIUM CHANNELS; CELL DIFFERENTIATION; CELLS, CULTURED; ELECTROPHYSIOLOGY; HUMANS; MESENCHYMAL STEM CELLS; MYOCYTES, CARDIAC; POTASSIUM CHANNELS;

EID: 33845748551     PISSN: 10010602     EISSN: 17487838     Source Type: Journal    
DOI: 10.1038/sj.cr.7310116     Document Type: Article

References (53)

1. Barry FP, Murphy JM. Mesenchymal stem cells: clinical applications and biological characterization. Int J Biochem Cell Biol 2004; 36:568-584.
2. Orlic D, Kajstura J, Chimenti S, Bodine DM, Leri A, Anversa P. Bone marrow cells regenerate infarcted myocardium. Nature 2001; 410:701-705.
3. Jackson KA, Majka SM, Wang H, et al. Regeneration of ischemic cardiac muscle and vascular endothelium by adult stem cells. J Clin Invest 2001; 107:1395-1402.
4. Strauer BE, Brehm M, Zeus T, et al. Repair of infarcted myocardium by autologous intracoronary mononuclear bone marrow cell transplantation in humans. Circulation 2002; 106:1913-1918.
5. Wollert KC, Drexler H. Clinical applications of stem cells for the heart. Circ Res 2005; 96:151-163.
6. Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999; 284:143-147.
7. Taylor SM, Jones PA. Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine. Cell 1979; 17:771-779.
8. Konieczny SF, Emerson CP Jr. 5-Azacytidine induction of stable mesodermal stem cell lineages from 10T1/2 cells: evidence for regulatory genes controlling determination. Cell 1984; 38:791-800.
9. Makino S, Fukuda K, Miyoshi S, et al. Cardiomyocytes can be generated from marrow stromal cells in vitro. J Clin Invest 1999; 103:697-705.
10. Boheler KR, Czyz J, Tweedie D, Yang HT, Anisimov SV, Wobus AM. Differentiation of pluripotent embryonic stem cells into cardiomyocytes. Circ Res 2002; 91:189-201.
11. Maltsev VA, Wobus AM, Rohwedel J, Bader M, Hescheler J. Cardiomyocytes differentiated in vitro from embryonic stem cells developmentally express cardiac-specific genes and ionic currents. Circ Res 1994; 75:233-244.
12. 2+ channel blockers. Differentiation 1991; 48:173-182.
13. Liu Y, Song J, Liu W, Wan Y, Chen X, Hu C. Growth and differentiation of rat bone marrow stromal cells: does 5-azacytidine trigger their cardiomyogenic differentiation? Cardiovasc Res 2003; 58:460-468.
14. Xu W, Zhang X, Qian H, et al. Mesenchymal stem cells from adult human bone marrow differentiate into a cardiomyocyte phenotype in vitro. Exp Biol Med (Maywood) 2004; 229:623-631.
15. Cao F, Niu LL, Meng L, et al. Cardiomyocyte-like differentiation of human bone marrow mesenchymal stem cells after exposure of 5-azacytidine in vitro. Shi Yan Sheng Wu Xue Bao 2004; 37:118-124.
16. Balana B, Nicoletti C, Zahanich I, et al. 5-Azacytidine treatment of mesenchymal stem cells influences potassium outward current density, but does not trigger cardiac differentiation. Naunyn-Schmiedeberg's Arch Pharmacol 2005; 371(Suppl 1):R16.
17. World Medical Association. World Medical Association Declaration of Helsinki. Ethical principles for medical research involving human subjects. Nurs Ethics 2002; 9:105-109.
18. Heubach JF, Graf EM, Leutheuser J, et al. Electrophysiological properties of human mesenchymal stem cells. J Physiol (London) 2004; 554:659-672.
19. Bruder SP, Jaiswal N, Haynesworth SE. Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. J Cell Biochem 1997; 64:278-294.
20. Dieckmann-Schuppert A, Schnittler HJ. A simple assay for quantification of protein in tissue sections, cell cultures, and cell homogenates, and of protein immobilized on solid surfaces. Cell Tissue Res 1997; 288:119-126.
21. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch 1981; 391:85-100.
22. Fabiato A, Fabiato F. Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J Physiol (Paris) 1979; 75:463-505.
23. Barry PH. JPCalc, a software package for calculating liquid junction potential corrections in patch-clamp, intracellular, epithelial and bilayer measurements and for correcting junction potential measurements. J Neurosci Methods 1994; 51:107-116.
24. 2+ currents coexist in guinea pig ventricular myocytes and are both blocked by mibefradil. Circ Res 2000; 86:628-635.
25. Bechem M, Schramm M. Calcium-agonists. J Mol Cell Cardiol 1987; 19(Suppl 2):63-75.
26. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162:156-159.
27. Katz AJ, Tholpady A, Tholpady SS, Shang H, Ogle RC. Cell surface and transcriptional characterization of human adipose-derived adherent stromal (hADAS) cells. Stem Cells 2005; 23:412-423.
28. 2+ channel in persistent atrial fibrillation in humans. Z Kardiol 2000; 89(Suppl 4):IV/23-IV/29.
29. Nakamura TM, Morin GB, Chapman KB, et al. Telomerase catalytic subunit homologs from fission yeast and human. Science 1997; 277:955-959.
30. Postma AV, Bezzina CR, de Vries JF, Wilde AA, Moorman AF, Mannens MM. Genomic organisation and chromosomal localisation of two members of the KCND ion channel family, KCND2 and KCND3. Hum Genet 2000; 106:614-619.
31. Senger M, Flores T, Glatting K Ernst P, Hotz-Wagenblatt A, Suhai S. W2H: WWW interface to the GCG sequence analysis package. Bioinformatics 1998; 14:452-457.
32. Perez-Iratxeta C, Palidwor G, Porter CJ, et al. Study of stem cell function using microarray experiments. FEBS Lett 2005; 579:1795-1801.
33. Alison MR. Tissue-based stem cells: ABC transporter proteins take centre stage. J Pathol 2003; 200:547-550.
34. Lassar A, Münsterberg A. Wiring diagrams: regulatory circuits and the control of skeletal myogenesis. Curr Opin Cell Biol 1994; 6:432-442.
35. Majumdar MK, Banks V, Peluso DP, Morris EA. Isolation, characterization, and chondrogenic potential of human bone marrow-derived multipotential stromal cells. J Cell Physiol 2000; 185:98-106.
36. Niwa H, Miyazaki J, Smith AG. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet 2000; 24:372-376.
37. Pochampally RR, Smith JR, Ylostalo J, Prockop DJ. Serum deprivation of human marrow stromal cells (hMSCs) selects for a subpopulation of early progenitor cells with enhanced expression of OCT-4 and other embryonic genes. Blood 2004; 103:1647-1652.
38. Parsch D, Fellenberg J, Brummendorf TH, Eschlbeck AM, Richter W. Telomere length and telomerase activity during expansion and differentiation of human mesenchymal stem cells and chondrocytes. J Mol Med 2004; 82:49-55.
39. Zimmermann S, Voss M, Kaiser S, Kapp U, Waller CF, Martens UM. Lack of telomerase activity in human mesenchymal stem cells. Leukemia 2003; 17:1146-1149.
40. Takeda Y, Mori T, Imabayashi H, et al. Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation? J Gene Med 2004; 6:833-845.
41. Zipori D. The stem state: plasticity is essential, whereas self-renewal and hierarchy are optional. Stem Cells 2005; 23:719-726.
42. Zhao P, Ise H, Hongo M, Ota M, Konishi I, Nikaido T. Human amniotic mesenchymal cells have some characteristics of cardiomyocytes. Transplantation 2005; 79:528-535.
43. Razin A, Kantor B. DNA methylation in epigenetic control of gene expression. Prog Mol Subcell Biol 2005; 38:151-167.
44. Taylor SM, Jones PA. Mechanism of action of eukaryotic DNA methyltransferase. Use of 5-azacytosine-containing DNA. J Mol Biol 1982; 162:679-692.
45. Oh H, Bradfute SB, Gallardo TD, et al. Cardiac progenitor cells from adult myocardium: homing, differentiation, and fusion after infarction. Proc Natl Acad Sci USA 2003; 100:12313-12318.
46. Rangappa S, Fen C, Lee EH, Bongso A, Sim EK. Transformation of adult mesenchymal stem cells isolated from the fatty tissue into cardiomyocytes. Ann Thorac Surg 2003; 75:775-779.
47. Chabner BA, Drake JC, Johns DG. Deamination of 5-azacytidine by a human leukemia cell cytidine deaminase. Biochem Pharmacol 1973; 22:2763-2765.
48. Li GR, Sun H, Deng X, Lau CP. Characterization of ionic currents in human mesenchymal stem cells from bone marrow. Stem Cells 2005; 23:371-382.
49. Goldberg JA, Wilson CJ. Control of spontaneous firing patterns by the selective coupling of calcium currents to calcium-activated potassium currents in striatal cholinergic interneurons. J Neurosci 2005; 25:10230-10238.
50. + channels designed for high-frequency repetitive firing. Trends Neurosci 2001; 24: 517-526.
51. Gessner G, Heinemann SH. Inhibition of hEAG1 and hERG1 potassium channels by clofilium and its tertiary analogue LY97241. Br J Pharmacol 2003; 138:161-171.
52. + channel. Receptors Channels 2001; 7:345-356.
53. + channels in breast cancer cells. Biochem Biophys Res Commun 2004; 316:244-251.