T-type and tetrodotoxin-sensitive CA2+ currents coexist in guinea pig ventricular myocytes and are both blocked by mibefradil

Circulation Research

Volumn 86, Issue 6, 2000, Pages 628-635

  Heubach, Jürgen F ^a   Köhler, Arndt ^a   Wettwer, Erich ^a   Ravens, Ursula ^a,b  

^a UNIVERSITY HOSPITAL CARL GUSTAV CARUS   (Germany)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Guinea pig ventricular myocytes; Mibefradil; T type Ca2+ currents; Tetrodotoxin sensitive Ca2+ currents

Indexed keywords

CALCIUM CHANNEL; MIBEFRADIL; NICKEL; TETRODOTOXIN;

ANIMAL CELL; ARTICLE; CALCIUM CONDUCTANCE; CALCIUM CURRENT; CALCIUM TRANSPORT; CELL ACTIVATION; CONTROLLED STUDY; ELECTROPHYSIOLOGY; GUINEA PIG; HEART MUSCLE CELL; HEART VENTRICLE WALL; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; STEADY STATE;

EID: 0343674552     PISSN: 00097330     EISSN: None     Source Type: Journal    
DOI: 10.1161/01.RES.86.6.628     Document Type: Article

References (30)

1. Bean BP. Two kinds of calcium channels in canine atrial cells: differences in kinetics, selectivity, and pharmacology. J Gen Physiol. 1985;86:1-30.
2. Nilius B, Hess P, Lansman JB, Tsien RW. A novel type of cardiac calcium channel in ventricular cells. Nature. 1985;316:443-446.
3. Marban E, Yamagishi T, Tomaselli GF. Structure and function of voltage-gated sodium channels. J Physiol (Lond). 1998;508:647-657.
4. Hagiwara N, Irisawa H, Kameyama M. Contribution of two types of calcium currents to the pacemaker potentials of rabbit sino-atrial node cells. J Physiol (Lond). 1988;395:233-253.
5. Santoro B, Liu DT, Yao H, Bartsch D, Kandel ER, Siegelbaum SA, Tibbs GR. Identification of a gene encoding a hyperpolarization-activated pacemaker channel of brain. Cell. 1998;93:717-729.
6. Ludwig A, Zong X, Jeglitsch M, Hofmann F, Biel M. A family of hyperpolarization-activated mammalian cation channels. Nature. 1998; 393:587-591.
7. Perez-Reyes E, Cribbs L, Daud A, Lacerda AE, Barclay J, Williamson MP, Fox M, Rees M, Lee JH. Molecular characterization of a neuronal low-voltage activated T-type calcium channel. Nature. 1998;391: 896-900.
8. 2+ channel gene family. Circ Res. 1998;83:103-109.
9. Lee JH, Daud AN, Cribbs LL, Lacerda AE, Pereverzev A, Klöckner U, Schneider T, Perez-Reyez E. Cloning and expression of a novel member of the low voltage activated T-type calcium channel family. J Neurosci. 1999;19:1912-1921.
10. Xu X, Best PM. Postnatal changes in T-type calcium current density in rat atrial myocytes. J Physiol (Lond). 1992;454:657-672.
11. Xu X, Best PM. Increase in T-type calcium current in atrial myocytes from adult rats with growth hormone-secreting tumors. Proc Natl Acad Sci U S A. 1990;87:4655-4659.
12. 2+ current is expressed in hypertrophic adult feline left ventricular myocytes. Circ Res. 1993;73:777-782.
13. 2+ channels are abnormal in genetically determined cardiomyopathic hamster hearts. Circ Res. 1994;75:149-155.
14. Brown AM, Lee KS, Powell T. Sodium currents in single rat heart muscle cells. J Physiol (Lond). 1981;318:479-500.
15. Heinemann SH, Terlau H, Stühmer W, Imoto K, Numa S. Calcium channel characteristics conferred on the sodium channel by single mutations. Nature. 1992;356:441-443.
16. + channels: slip-mode conductance. Science. 1998;279: 1027-1033.
17. 2+ currents in human atrial cells. Receptors Channels. 1995;3:71-81.
18. Aggarwal R, Shorofsky SR, Goldman L, Balke CW. Tetrodotoxin-blockable calcium currents in rat ventricular myocytes: a third type of cardiac cell sodium current. J Physiol (Lond). 1997;505:353-369.
19. + channels in guinea pig ventricular myocytes. Am J Physiol. 1997; 273:H128-H137.
20. Nuss HB, Marban E; Balke CW, Goldman L, Aggarwal R, Shorofsky SR. Whether 'slip-mode conductance' occurs. Science. 1999;284:711.
21. Tytgat J, Vereecke J, Carmeliet E. A combined study of sodium current and T-type calcium current in isolated cardiac cells. Pflugers Arch. 1990;417:142-148.
22. 2-adrenoceptor. Cardiovasc Res. 1998;42:173-182.
23. Fabiato A, Fabiato F. Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J Physiol (Paris). 1979;75:463-505.
24. Barry PH. JPCalc, a software package for calculating liquid junction potential corrections in patch-clamp, intracellular, epithelial and bilayer measurements and for correcting junction potential measurements. J Neurosci Methods. 1994;51:107-116.
25. Cohen CJ, Spires S, Van Skiver D. Block of T-type Ca channels in guinea pig atrial cells by antiarrhythmic agents and Ca channel antagonists. J Gen Physiol. 1992;100:703-728.
26. 2+ channels in guinea-pig heart cells. J Physiol (Lond). 1992;456:247-265.
27. Mitra R, Morad M. Two types of calcium channels in guinea pig ventricular myocytes. Proc Natl Acad Sci U S A. 1986;83:5340-5344.
28. + current in human ventricular myocytes. Circ Res. 1996;78:689-696.
29. Kostyuk PG, Mironov SL, Doroshenko PA, Ponomarev VN. Surface charges on the outer side of mollusc neuron membrane. J Membr Biol. 1982;70:171-179.
30. 2+ channels and pharmacological blockade: potential pathophysiological relevance. Cardiovasc Drugs Ther. 1997;11:723-739.