Mass spectrometric evidence of covalently-bound tetrahydrolipstatin at the catalytic serine of Streptomyces rimosus lipase

Biochimica et Biophysica Acta - General Subjects

Volumn 1770, Issue 2, 2007, Pages 163-170

  Ašler, Ivana Leščić ^a   Zehl, Martin ^b   Kovačić, Filip ^a   Müller, Roland ^b   Abramić, Marija ^a   Allmaier, Günter ^b   Kojić Prodić, Biserka ^a  

^a RUDER BO KOVI INSTITUTE   (Croatia)

^b VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Capillary gel electrophoresis on the chip; Covalent inhibition; ESI mass spectrometry; Kinetics; MALDI tandem mass spectrometry; Streptomyces rimosus, Extracellular SGNH lipase; Tetrahydrolipstatin

Indexed keywords

2 PROPANOL; ESTER DERIVATIVE; FUNGAL ENZYME; SERINE; TETRAHYDROLIPSTATIN; TRIACYLGLYCEROL LIPASE; WATER;

AQUEOUS SOLUTION; ARTICLE; COLLISIONALLY ACTIVATED DISSOCIATION; COVALENT BOND; ENERGY; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME KINETICS; ENZYME STABILITY; INCUBATION TIME; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; PH; PRIORITY JOURNAL; STREPTOMYCES; STREPTOMYCES RIMOSUS; TANDEM MASS SPECTROMETRY; THERMOSTABILITY; VACUUM;

BACTERIAL PROTEINS; BINDING SITES; CATALYTIC DOMAIN; ELECTROPHORESIS, CAPILLARY; LACTONES; LIGASES; SERINE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STREPTOMYCES; TRYPSIN;

STREPTOMYCES RIMOSUS;

EID: 33845690506     PISSN: 03044165     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbagen.2006.10.011     Document Type: Article

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