The open lid mediates pancreatic lipase function

Journal of Lipid Research

Volumn 41, Issue 1, 2000, Pages 48-57

  Yang, Yanqing ^a   Lowe, Mark E ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Bile salts; Colipase; Pancreatic lipase related protein; Site directed mutagenesis

Indexed keywords

BILE SALT; COLIPASE; PHOSPHOLIPASE; TRIACYLGLYCEROL LIPASE; TRIACYLGLYCEROL LIPASE RELATED PROTEIN 2; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME STABILITY; LIPOLYSIS; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

AMINO ACID SEQUENCE; ENZYME ACTIVATION; LIPASE; LIPOLYSIS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PANCREAS; PHOSPHOLIPASES; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 0033971766     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Hide, W. A., L. Cham and W-H. Li. 1992. Structure and evolution of the lipase superfamily. J. Lipid Res. 33: 167-178.
2. Verger, R. 1984. Pancreatic lipase. In Lipases. B. Borgstrom, and H. L. Brockman, editors. Elsevier, Amsterdam. 84-150.
3. Giller, T., P. Buchwald, D. Blum-Kaelin, and W. Hunziker. 1992. Two novel human pancreatic lipase related proteins, hPLRP1 and hPLRP2: differences in colipase dependency and in lipase activity. J. Biol. Chem. 267: 16509-16516.
4. Hjorth, A., F. Carriere, C. Cudrey, H. Woldike, E. Boel, D. M. Lawson, F. Ferrato, C. Cambillau, G. G. Dodson, L. Thim, and R. Verger. 1993. A structural domain (the lid) found in pancreatic lipase is absent in the guinea pig (phospho)lipase. Biochemistry. 32: 4702-4707.
5. Jennens, M. L., and M. E. Lowe. 1995. Rat GP-3 is a pancreatic lipase with kinetic properties that differ from colipase-dependent pancreatic lipase. J. Lipid Res. 36: 2374-2381.
6. Thirstrup, K., R. Verger, and F. Carriere. 1994. Evidence for a pancreatic lipase subfamily with new kinetic properties. Biochemistry. 33: 2748-2756.
7. Andersson, L., F. Carriere, M. E. Lowe, A. Nilsson, and R. Verger. 1996. Pancreatic lipase-related protein 2 but not classical pancreatic lipase hydrolyzes galactolipids. Biochim. Biophys. Acta. 1302: 236-240.
8. Roussel, A., Y. Yang, F. Ferrato, R. Verger, C. Cambillau, and M. E. Lowe. 1998. Structure and activity of rat pancreatic lipase related protein 2. J. Biol. Chem. 273: 32121-32128.
9. Winkler, F. K., A. D'Arcy, and W. Hunziker. 1990. Structure of human pancreatic lipase. Nature. 343: 771-774.
10. Brzozowski, A. M., U. Derewenda, Z. S. Derewenda, G. G. Dodson, D. M. Lawson, J. P. Turkenburg, F. Bjorkling, B. Huge-Jensen, S. A. Patkar, and L. Thim. 1991. A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex. Nature. 251: 491-494.
11. van Tilbeurgh, H., L. Sarda, R. Verger, and C. Cambillau. 1992. Structure of the pancreatic lipase-procolipase complex. Nature. 359: 159-162.
12. van Tilbeurgh, H., M. P. Egloff, C. Martinez, N. Rugani, R. Verger, and C. Cambillau. 1993. Interfacial activation of the lipase-procolipase complex by mixed micelles revealed by X-ray crystallography. Nature. 362: 814-820.
13. Dugi, K. A., H. L. Dichek, G. D. Talley, H. B. Brewer, and S. Santamarina-Fojo. 1992. Human lipoprotein lipase: the loop covering the catalytic site is essential for interaction with lipid substrates. J. Biol. Chem. 267: 25086-25091.
14. Dugi, K. A., H. L. Dichek, and S. Santamarina-Fojo. 1995. Human hepatic and lipoprotein lipase: The loop covering the catalytic site mediates lipase substrate specificity. J. Biol. Chem. 270: 25396-25401.
15. Faustinella, F., L. C. Smith, and L. Chan. 1992. Functional topology of a surface loop shielding the catalytic center in lipoprotein lipase. Biochemistry. 31: 7219-7223.
16. Carriere, F., K. Thirstrup, S. Hjorth, F. Ferrato, P. F. Nielsen, C., Withers-Martinez, C. Cambillau, E. Boel, L. Thim, and R. Verger. 1997. Pancreatic lipase structure-function relationships by domain exchange. Biochemisty. 36: 239-248.
17. Jennens, M. L., and M. E. Lowe. 1994. A surface loop covering the active site of human pancreatic lipase influences interfacial activation and lipid binding. J. Biol. Chem. 269: 25470-25474.
18. Lowe, M. 1997. Colipase stabilizes the lid domain of pancreatic triglyceride lipase. J. Biol. Chem. 272: 9-12.
19. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
20. Higuchi, R., B. Krummel, and R. K. Saiki. 1988. A general method of in vitro preparation and specific mutations of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res. 16: 7351-7367.
21. Payne, R. M., H. F. Sims, M. L. Jennens, and M. E. Lowe. 1994. Rat pancreatic lipase and two related proteins: enzymatic properties and mRNA expression during development. Am. J. Physiol. 266: G914-G921.
22. Yang, Y., and M. Lowe. 1998. Human pancreatic triglyceride lipase expressed in yeast cells: Purification and characterization. Protein Expr. Purif. 13: 36-40.
23. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Anal. Biochem. 72: 248-254.
24. Cordle, R. A., and M. E. Lowe. 1998. Purification and characterization of human procolipase expressed in yeast cells. Protein Expr. Purif. 13: 30-35.
25. Lowe, M. E. 1998. Assays for pancreatic triglyceride lipase and colipase. In Methods in Molecular Biology: Lipase and Phopholipase Protocols. Vol. 109. M. H. Doolittle, and K. Reue, editors. Humana Press Inc., Totowa, NJ. 59-70.
26. Lister, M. D., R. A. Deems, Y. Watanabe, R. J. Ulevitch, and E. A. Dennis. 1988. Kinetic analysis of the Ca-dependent, membrane-bound, macrophage phospholipase A2 and the effects of arachidonic acid. J. Biol. Chem. 263: 7506-7513.
27. Martinelle, M., M. Holmquist, and K. Hult. 1995. On the interfacial activation of Candida antartica lipase A and B as compared with Humicola lanuginosa lipase. Biochim. Biophys. Acta. 1258: 272-276.
28. Derewenda, U., A. M. Brzozowski, D. M. Lawson, and Z. S. Derewenda. 1992. Catalysis at the interface: the anatomy of a conformational change in a triglyceride lipase. Biochemistry. 31: 1532-1541.
29. Egloff, M. P., F. Marguet, G. Buono, R. Verger, C. Cambillau, and H. van Tilbeurgh. 1995. The 2.46 A resolution of the pancreatic lipase-colipase complex inhibited by a C11 alkyl phosphonate. Biochemistry. 24: 2751-2762.
30. Lowe, M. E. 1997. New pancreatic lipases: gene expression, protein secretion, and the newborn. Methods Enzymol. 284: 285-297.