Purification and kinetic characterization of a fructosyltransferase from Aspergillus aculeatus

Journal of Biotechnology

Volumn 128, Issue 1, 2007, Pages 204-211

  Ghazi, Iraj ^a   Fernandez Arrojo, Lucia ^a   Garcia Arellano, Humberto ^a   Ferrer, Manuel ^a   Ballesteros, Antonio ^a   Plou, Francisco J ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

Author keywords

Fructofuranosidase; Aspergillus aculeatus; Fructooligosaccharides; Fructosyltransferase; Transfructosidase

Indexed keywords

CONCENTRATION (PROCESS); FUNGI; PURIFICATION; REACTION KINETICS; SUBSTRATES;

Β-FRUCTOFURANOSIDASE; ASPERGILLUS ACULEATUS; FRUCTOOLIGOSACCHARIDES; FRUCTOSYLTRANSFERASE; TRANSFRUCTOSIDASE;

ENZYMES;

1 KESTOSE; DODECYL SULFATE SODIUM; FRUCTOSE OLIGOSACCHARIDE; FRUCTOSYLNYSTOSE; FRUCTOSYLTRANSFERASE; FUNGAL ENZYME; GLYCOPROTEIN; NYSTOSE; POLYSORBATE 80; PREBIOTIC AGENT;

ARTICLE; ASPERGILLUS; ASPERGILLUS ACULEATUS; CONCENTRATION RESPONSE; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STABILITY; HYDROLYSIS; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONTENT;

ASPERGILLUS; FOOD ADDITIVES; HEXOSYLTRANSFERASES; SUCROSE;

ASPERGILLUS ACULEATUS;

EID: 33845611930     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.09.017     Document Type: Article

References (25)

1. Antosova M., and Polakovic M. Fructosyltransferases: the enzymes catalyzing production of fructooligosaccharides. Chem. Pap. Chem. Zvesti. 55 (2001) 350-358
2. Ballesteros A., Plou F.J., Alcalde M., Ferrer M., García-Arellano H., Reyes-Duarte D., and Ghazi I. Enzymatic synthesis of sugar esters and oligosaccharides from renewable resources. In: Patel R. (Ed). Biocatalysis in the Pharmaceutical and Biotechnological Industries (2006), CRC Press, New York 465-490
3. Boddy L.M., Berges T., Barreau C., Vainstein M.H., Dobson M.J., Ballance D.J., and Peberdy J.F. Purification and characterisation of an Aspergillus niger invertase and its DNA sequence. Curr. Genet. 24 (1993) 60-66
4. Chavez F.P., Rodriguez L., Diaz J., Delgado J.M., and Cremata J.A. Purification and characterization of an invertase from Candida utilis: comparison with natural and recombinant yeast invertases. J. Biotechnol. 53 (1997) 67-74
5. Fujishima M., Sakai H., Ueno K., Takahashi N., Onodera S., Benkeblia N., and Shiomi N. Purification and characterization of a fructosyltransferase from onion bulbs and its key role in the synthesis of fructo-oligosaccharides in vivo. New Phytol. 165 (2005) 513-524
6. Ghazi I., Gomez de Segura A., Fernandez-Arrojo L., Alcalde M., Yates M., Rojas-Cervantes M.L., Plou F.J., and Ballesteros A. Immobilisation of fructosyltransferase from Aspergillus aculeatus on epoxy-activated Sepabeads EC for the synthesis of fructo-oligosaccharides. J. Mol. Catal. B: Enzym. 35 (2005) 19-27
7. Ghazi I., Fernandez-Arrojo L., Gomez de Segura A., Alcalde M., Plou F.J., and Ballesteros A. Beet sugar syrup and molasses as low-cost feedstock for the enzymatic production of fructo-oligosaccharides. J. Agric. Food Chem. 54 (2006) 2964-2968
8. Hang Y.D., and Woodams E.E. Fructosyltransferase activity of commercial enzyme preparations used in fruit juice processing. Biotechnol. Lett. 17 (1995) 741-745
9. Hayashi S., Matsuzaki K., Takasaki Y., Ueno H., and Imada K. Purification and properties of β-fructofuranosidase from Aspergillus japonicus. World J. Microbiol. Biotechnol. 8 (1992) 155-159
10. Hirayama M., Sumi N., and Hidaka H. Purification and properties of a fructooligosaccharide-producing β-fructofuranosidase from Aspergillus niger ATCC 20611. Agric. Biol. Chem. 53 (1988) 667-673
11. Klein R.D., Poorman R.A., Favreau M.A., Shea M.H., Hatzenbuhler N.T., and Nulf S.C. Cloning and sequence analysis of the gene encoding invertase from the yeast Schwanniomyces occidentalis. Curr. Genet. 16 (1989) 145-152
12. Koops A.J., and Jonker H.H. Purification and characterization of the enzymes of fructan biosynthesis in tubers of Helianthus tuberosus "Columbia": fructan:fructan 1-fructosyltransferase. J. Exp. Bot. 45 (1994) 1623-1631
13. L'Hocine L., Wang Zh., Jiang B., and Xu S. Purification and partial characterization of fructosyltransferase and invertase from Aspergillus niger AS0023. J. Biotechnol. 81 (2000) 73-84
14. Mukasa H., Shimamura A., and Tsumori H. Direct activity stains for glycosidase and glycosyltransferase after isoelectric focusing in horizontal polyacrylamide gel layers. Anal. Biochem. 123 (1982) 276-284
15. Okai A.A.E., and Gierschner K. Size and charge properties of the pectic and cellulolytic enzymes in a commercial enzyme preparation. Z. Lebensm. Unters. Forsch. 192 (1991) 244-248
16. Park J.P., Oh T.K., and Yun J.W. Purification and characterization of a novel transfructosylating enzyme from Bacillus macerans EG-6. Process Biochem. 37 (2001) 471-476
17. Reddy A., and Maley F. Studies on identifying the catalytic role of Glu-204 in the active site of yeast invertase. J. Biol. Chem. 271 (1996) 13953-13957
18. Rubio M.C., Runco R., and Navarro A.R. Invertase from a strain of Rhodotorula glutinis. Phytochemistry 61 (2002) 605-609
19. F-fructosyltransferase from the roots of asparagus (Asparagus officinalis L.). Carbohydr. Res. 99 (1982) 157-169
20. Simmering R., and Blaut M. Pro- and prebiotics-the tasty guardian angels?. Appl. Microbiol. Biotechnol. 55 (2001) 19-28
21. Sumner J.B., and Howell S.F. A method for determination of saccharase activity. J. Biol. Chem. 108 (1935) 51-54
22. Tanriseven A., and Gokmen F. Novel method for the production of a mixture containing fructooligosaccharides and isomaltooligosaccharides. Biotechnol. Tech. 13 (1999) 207-210
23. Tanriseven A., and Aslan Y. Immobilization of Pectinex Ultra SP-L to produce fructooligosaccharides. Enzyme Microb. Technol. 36 (2005) 550-554
24. van Hijum S.A.F.T., Szalowska E., van der Maarel M.J.E.C., and Dijkhuizen L. Biochemical and molecular characterization of a levansucrase from Lactobacillus reuteri. Microbiology 150 (2004) 621-630
25. Yanai K., Nakane A., Kawate A., and Hirayama M. Molecular cloning and characterization of the fructooligosaccharide producing β-fructofuranosidase gene from Aspergillus niger ATCC 20611. Biosci. Biotechnol. Biochem. 65 (2001) 766-773