A real-time PCR method for quantifying viable Ascaris eggs using the first internally transcribed spacer region of ribosomal DNA

Applied and Environmental Microbiology

Volumn 72, Issue 12, 2006, Pages 7864-7872

  Pecson, Brian M ^a   Barrios, José Antonio ^b   Johnson, David R ^a   Nelson, Kara L ^a  

^a Berkeley   (United States)

^b UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; DISEASES; DNA; MICROORGANISMS; MICROSCOPIC EXAMINATION; SEWAGE SLUDGE; WASTEWATER;

ASCARIS EGGS; ASCARIS LUMBRICOIDES; LARVATED EGGS; MICROSCOPY METHOD;

REAL TIME SYSTEMS;

AMMONIA; INTERNAL TRANSCRIBED SPACER; RIBOSOME DNA; RIBOSOME RNA;

DETECTION METHOD; DNA; EGG; NEMATODE; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; VIABILITY;

ARTICLE; ASCARIS; ASCARIS LUMBRICOIDES; CELL COUNT; CELL VIABILITY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; EGG; HEAT TREATMENT; LARVA; MICROSCOPY; NONHUMAN; NUCLEOTIDE SEQUENCE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SLUDGE; STATISTICAL SIGNIFICANCE; ULTRAVIOLET RADIATION; WASTE WATER;

ANIMALS; ASCARIS SUUM; DNA PRIMERS; DNA, HELMINTH; DNA, RIBOSOMAL SPACER; OVUM; PARASITE EGG COUNT; POLYMERASE CHAIN REACTION; RNA, HELMINTH; RNA, RIBOSOMAL;

ASCARIS; ASCARIS LUMBRICOIDES;

EID: 33845546929     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01983-06     Document Type: Article

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