Study of 18S rRNA and rDNA stability by real-time RT-PCR in heat-inactivated Cryptosporidium parvum oocysts

FEMS Microbiology Letters

Volumn 226, Issue 2, 2003, Pages 237-243

  Fontaine, Melanie ^a   Guillot, Emmanuelle ^a  

^a CIRSEE   (France)

Author keywords

18S rRNA; Cryptosporidium parvum; Heat inactivation; Real time PCR; TaqMan

Indexed keywords

RIBOSOME DNA; RIBOSOME RNA; CHELATING AGENT; CHELEX 100; PROTOZOAL DNA; PROTOZOAL RNA; RESIN; RNA 18S; WATER;

ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIUM PARVUM; DEGRADATION; HEAT TOLERANCE; HEAT TREATMENT; NONHUMAN; NUCLEOTIDE SEQUENCE; OOCYST; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA PROBE; RNA STABILITY; ANIMAL; DISINFECTION; HEAT; ISOLATION AND PURIFICATION; METHODOLOGY; PARASITOLOGY; PHYSIOLOGY; RNA GENE; SENSITIVITY AND SPECIFICITY;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM;

ANIMALS; CHELATING AGENTS; CRYPTOSPORIDIUM PARVUM; DISINFECTION; DNA, PROTOZOAN; DNA, RIBOSOMAL; GENES, RRNA; HEAT; OOCYSTS; RESINS, SYNTHETIC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, PROTOZOAN; RNA, RIBOSOMAL, 18S; SENSITIVITY AND SPECIFICITY; WATER;

EID: 0642316223     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00538-X     Document Type: Article

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