An internal reference technique for accurately quantifying specific mRNAs by real-time PCR with application to the tceA reductive dehalogenase gene

Applied and Environmental Microbiology

Volumn 71, Issue 7, 2005, Pages 3866-3871

  Johnson, David R ^a   Lee, Patrick K H ^a   Holmes, Victor F ^a   Alvarez Cohen, Lisa ^a  

^a Berkeley   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CHLORINATION; ENZYMES; GENES; HYDROCARBONS; MICROBIOLOGY;

CELL LYSIS; REVERSE TRANSCRIPTION (RT); SAMPLE PROCESSING PROTOCOLS; TRICHLOROETHANE (TCE);

RNA;

1,2 DICHLOROETHYLENE; BACTERIAL ENZYME; DNA; ETHYLENE; MESSENGER RNA; REDUCTIVE DEHALOGENASE; TRICHLOROETHYLENE; UNCLASSIFIED DRUG; VINYL CHLORIDE;

POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; RNA;

ACCURACY; ARTICLE; BACTERIAL GENE; CHLORINATION; CLINICAL PROTOCOL; CONTROLLED STUDY; CYTOLYSIS; DNA EXTRACTION; GENE EXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; QUANTITATIVE ANALYSIS; QUANTITATIVE GENETICS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA ISOLATION; SAMPLE; TCEA GENE; TECHNIQUE;

ANAEROBIOSIS; BACTERIA; CULTURE MEDIA; DICHLOROETHYLENES; ECOSYSTEM; OXIDOREDUCTASES; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRICHLOROETHYLENE;

TRIXIS;

EID: 22144451860     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.7.3866-3871.2005     Document Type: Article

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