Pretreatment to avoid positive RT-PCR results with inactivated viruses

Journal of Virological Methods

Volumn 104, Issue 2, 2002, Pages 217-225

  Nuanualsuwan, Suphachai ^a   Cliver, Dean O ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Feline calicivirus; Hepatitis A virus; Inactivated viruses; Poliovirus; RT PCR

Indexed keywords

HYPOCHLORITE; PROTEINASE K; RIBONUCLEASE;

AMPLICON; ARTICLE; CALICIVIRUS; CONTROLLED STUDY; HEATING; HEPATITIS A VIRUS; NONHUMAN; NORWALK GASTROENTERITIS VIRUS; POLIOMYELITIS VIRUS 1; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ULTRAVIOLET RADIATION; VIRUS DETECTION; VIRUS INACTIVATION; VIRUS STRAIN;

ANIMALS; CALICIVIRUS, FELINE; CATS; CELL LINE; HEPATITIS A VIRUS; HUMANS; KIDNEY; MACACA MULATTA; MODELS, ANIMAL; NOROVIRUS; POLIOVIRUS; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; VACCINES, INACTIVATED; VIRAL VACCINES;

CALICIVIRIDAE; FELIDAE; FELINE CALICIVIRUS; HEPATITIS A VIRUS; HUMAN HEPATITIS A VIRUS; NOROVIRUS; POLIOVIRUS;

EID: 0036089796     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(02)00089-7     Document Type: Article

References (14)

1. Rapid detection of enterovirus in clinical specimens using PCR and microwell capture hybridization assay
2. Proteinase K stability
3. Detection of hepatitis A virus in environmental samples by antigen-capture polymerase chain reaction
4. Inactivation of feline calicivirus, a Norwalk virus surrogate
5. Immunomagnetic capture PCR for rapid concentration and detection of hepatitis A virus from environmental samples
6. Small round coliphages as surrogates for human viruses in process assessment
7. Food-related illness and death in the United States
8. Application of PCR to detect Norwalk virus in fecal specimens from outbreaks of gastroenteritis
9. Model for virus inactivation by chlorination
10. Limitations of molecular biological techniques for assessing the virological safety of foods
11. Effectiveness of hypochlorous acid and hypochlorite ion in destruction of viruses and bacteria
12. Feline calicivirus as a model system for heat inactivation studies of small round structured viruses in shellfish
13. Inactivation of cell-associated and dispersed hepatitis A virus in water