Greenbottle (Lucilia sericata)larval secretions delivered from a prototype hydrogel wound dressing accelerate the closure of model wounds

Biotechnology Progress

Volumn 22, Issue 6, 2006, Pages 1690-1696

  Smith, Annie G ^a   Powis, Rachel A ^a   Pritchard, David I ^c   Britland, Stephen T ^a,b  

^a UNIVERSITY OF BRADFORD   (United Kingdom)

^b AGT Sciences Ltd   (United Kingdom)

^c UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; ENZYME INHIBITION; HYDROGELS; IMAGE ANALYSIS; TISSUE;

CONTROLLED DELIVERY; EPITHELIAL CELLS; HISTOCHEMICAL ANALYSIS; TISSUE REGENERATION;

BIOCHEMISTRY;

DRUG CARRIER; ENZYME;

ANIMAL; ARTICLE; BANDAGE; CELL STRAIN 3T3; CHEMISTRY; DISEASE MODEL; DRUG EFFECT; ENZYMOLOGY; FLY; HYDROGEL; LARVA; MOUSE; PILOT STUDY; WOUND HEALING;

3T3 CELLS; ANIMALS; BANDAGES; DIPTERA; DISEASE MODELS, ANIMAL; DRUG CARRIERS; ENZYMES; HYDROGELS; LARVA; MICE; PILOT PROJECTS; WOUND HEALING;

LUCILIA SERICATA;

EID: 33845481174     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp0601600     Document Type: Article

References (40)

1. Aota, S.; Nomizu, M.; Yamada, K. M. The short amino acid sequence Pro-His-Ser-Arg-Asn in human fibronectin enhances cell-adhesive function. J. Biol. Chem. 1994, 269, 24756-24761.
2. Beasley, W. D.; Hirst, G. Making a meal of MRSA - the role of biosurgery in hospital-acquired infection. J. Hosp. Inf. 2004, 56, 6-9.
3. Brown, L. F.; Lanir, N.; McDonagh, J.; Tognazzi, K.; Dvorak, A. M.; Dvorak, H. F. Fibroblast migration in fibrin gel matrices. Am. J. Pathol. 1993, 42, 273-283.
4. Carmichael J.; DeGraff W. G.; Gazdar A. F.; Minna, J. D.; Mitchell, J. B. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 1987, 147, 936-942.
5. Casu, R. E.; Jarmey, J. M.; Elvin, C. M.; Eisemann, C. H. Isolation of a trypsin-like serine protease gene family from the sheep blowfly Lucilia cuprina. Insect Mol. Biol. 1994, 3, 159-170.
6. Chambers L.; Woodrow S.; Brown A. P.; Harris P. D.; Phillips D.; Hall M.; Church J. C. T.; Pritchard, D. I. Degradation of extracellular matrix components by defined proteases from the greenbottle larva Lucilia sericata used for the clinical debridement of non-healing wounds. Br. J Dermatol. 2003, 148, 14-23.
7. Chapman, H. A. Plasminogen activators, integrins, and the coordinated regulation of cell adhesion and migration. Curr. Opin. Cell Biol. 1997, 9, 714-724.
8. Cottrell, C. S.; Coelho, A. M.; Bunnett, N. W. Protease-activated receptors: the role of cell surface proteolysis in cell signaling. Essays Biochem. 2002, 38, 169-183.
9. Etscheid, M.; Beer, N.; Dodt, J. The hyaluronan-binding protease upregulates ERK1/2 and PI3K/Akt signalling pathways in fibroblasts and stimulates cell proliferation and migration. Cell Biol. 2005, 17, 1486-1494.
10. Evans, P. Larvae therapy and venous leg ulcers: reducing the "yuk factor". J. Wound Care. 2002, 11, 407-408.
11. Grassberger, M.; Franks, C. Wound healing by steril fly larvas: basic mechanical, biochemical and microbiological principles. Wien. Med. Wochenschr. 2003, 153, 198-201.
12. Greener, B.; Hughes, A. A.; Banister, N. P.; Douglass, J. Proteases and pH in chronic wounds. J. Wound Care. 2005, 14, 59-61.
13. Hennink W. E.; Van Nostrum, C. F. Novel crosslinking methods to design hydrogels. Adv. Drug Deliv. Rev. 2002, 54, 13-36.
14. Hobson, R. P. On an enzyme from blowfly larvae. (Lucilia Sericata) which digests collagen in alkaline solution. Biochem. J. 1931, 25, 1458.
15. Hoffman, A. S. Hydrogels for biomedical applications. Adv. Drug Deliv. Rev. 2002, 43, 3-12.
16. Horobin, A. J; Shakesheff, K. M.; Woodrow, S.; Robinson, C.; Pritchard, D. I. (2003) Maggots and wound healing: The effects of Lucilia sericata larval secretions upon human dermal fibroblasts. Br. J. Dermatol. 2003, 148, 923-933.
17. Horobin, A. J.; Shakesheff K. M.; Pritchard, D. I. Maggots and wound healing: an investigation of the effects of secretions from Lucilia sericata Larvae upon the migration of human dermal fibroblasts over a fibronectin-coated surface. Wound Rep. Reg. 2005, 13, 422-433.
18. Katz, B. Z.; Zamir, E.; Bershadsky, A.; Kam, Z.; Yamada, K. M.; Geiger, B. Physical state of the extracellular matrix regulates the structure and molecular composition of cell-matrix adhesions. Mol. Biol. Cell. 2000, 11, 1047-1060.
19. Khalili, A. S.; Domogatskii, S. P.; Blitzniukov, O. P.; Ruuge, E. K. Treatment of burn surfaces by proteinases: mathematical description of an enzyme distribution. Biophizika 2003, 48, 76-83.
20. Livingston, S. K. The therapeutic active principle of maggots with a description of its clinical application in 567 cases. J. Bone Joint Surg. 1936,18, 751-756.
21. Malinda, K. M.; Sidhu, G. S.; Banaudha, K. K.; Gaddipati, J. P.; Maheshwari, P. K.; Goldstein, A. L.; Kleinman, H. K. Thymosin alpha 1 stimulates endothelial cell migration, angiogenesis and wound healing. J. Immunol. 1998, 160, 1001-1006.
22. Marquardt, C.; von Seydlitz-Kurzbach, J.; Hoefer, D. How long should biosurgical maggots be kept in the wound for maggot debridement therapy (MDT)? An experimental investigation of the age depentent enzymatic secretion in larvae of Lucilia sericata. EWMA J. 2005, P70, V26-1.
23. Mitra, S. K.; Hanson, D. A.; Schlaepfer, D. D. Focal adhesion kinase: in command and control of cell motility. Nat. Rev. Mol. Cell Biol. 2005, 6, 56-68.
24. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65, 55-63.
25. Mumcuoglu, K. Y.; Ingber, A.; Gilead, L.; Stessman, J.; Friedmann, R.; Schulman, H.; Bichucher, H.; Ioffe-Uspensky, I.; Miller, J.; Galun, R.; Raz, I. Maggot therapy for the treatment of intractable wounds. Int. J. Dermatol. 1999, 38, 623-627.
26. Mumcuoglu, K. Y. Clinical applications for maggots in wound care. Am. J. Clin. Dermatol. 2001, 2, 219-227.
27. Novak, J. F.; Turnka, F. Proenzyme therapy of cancer. Anticancer Res. 2005, 25, 1157-1177.
28. Palecek, S. P.; Loftus, J. C.; Ginsberg, M. H.; Lauffenburger, D. A.; Horwitz, A. F. Integrin-ligand binding properties govern cell migration speed through cell-substratum adhesiveness. Nature 1997, 385, 537-540.
29. Prete, P. E. Growth effects of Phaenicia sericata larval extracts on fibroblasts: mechanism for wound healing by maggot therapy. Life Sci. 1997, 60, 505-510.
30. Stadelmann, W. K.; Digenis, A. G.; Tobin, G. R. Physiology and healing dynamics of chronic cutaneous wounds. Am. J. Surg. 1998, 176, 26S-38S.
31. Thomas, S.; Andrews, A. M.; Hay, N. P.; Bourgoise, S. The antimicrobial activity of maggot secretions: results of a preliminary study. J. Tiss. Viab. 1999, 9, 127-132.
32. Wilson, E. H.; Doan, C. A.; Miller, D. F. The Baer maggot treatment of osteomyelitis - Preliminary report of 26 cases. J. Am. Med. Assoc. 1932, 98, 1149-1152.
33. Palka, J.; Adelmann-Grill, B. C.; Francz, P. I.; Bayreuther, K. Differentiation stage and cell cycle position determine the chemotactic response of fibroblasts. Folia Histochem. Cytobiol. 1996, 34, 121-127.
34. Walker, D. C.; Hill, G.; Wood, S. M.; Smallwood, R. H.; Southgate, J. Agent-based computational modelling of epithelial cell monolayers: predicting the effect of exogenous calcium concentration on the rate of wound closure. Trans. Nanobiosci. 2004, 3, 153-163.
35. Wollina, U.; Liebold, K.; Schmidt, W. D.; Hartmann, M.; Fassler, D. Biosurgery supports granulation and debridement in chronic wounds - clinical data and remittance spectroscopy measurement. Int. J. Dermatol. 2002, 41, 635-639.
36. Sherman, R. A.; Hall, M. J.; Thomas, S. Medicinal maggots: an ancient remedy for some contemporary afflictions. Annu. Rev. Entemol. 2000, 45, 55-81.
37. Akiyama, S. K.; Yamada, S. S.; Chen, W. T.; Yamada, K. M. Analysis of fibronectin receptor function with monoclonal antibodies: roles in cell adhesion, migration, matrix assembly, and cytoskeletal organization. J. Cell. Biol. 1989, 109, 863-875.
38. Buth, H.; Wolters, B.; Hartwig, B.; Meier-Bornheim, R.; Veith, H.; Hansen, M.; Sommerhoff, C. P.; Schaschke, N.; Machleidt, W.; Fusenig, N. E.; Boukamp, P.; Brix, K. HaCaT keratinocytes secrete lysosomal cysteine proteinases during migration. Eur. J. Cell Biol. 2004, 83, 781-795.
39. Sherman, R. A. Maggot versus conservative debridement therapy for the treatment of pressure ulcers. Wound Repair Regen. 2002, 10, 208-214.
40. Sherman, R. A. Maggot therapy for treating diabetic foot ulcers unresponsive to conventional therapy. Diabetes Care 2003, 26, 446-451.