Development of a simple high-throughput screening protocol based on biosynthetic activity of Mycobacterium tuberculosis glutamine synthetase for the identification of novel inhibitors

Journal of Biomolecular Screening

Volumn 11, Issue 8, 2006, Pages 1035-1042

  Singh, Upasana ^a   Sarkar, Dhiman ^a  

^a NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

Beckman Coulter; Biosynthetic activity; Glutamine synthetase; High throughput screening; Mycobacterium tuberculosis

Indexed keywords

ADENOSINE TRIPHOSPHATE; AMMONIUM CHLORIDE; BACTERIAL ENZYME; DIMETHYL SULFOXIDE; ENZYME INHIBITOR; GLUTAMATE AMMONIA LIGASE; GLUTAMIC ACID; GLUTAMINE; MAGNESIUM CHLORIDE; METHIONINE SULFOXIMINE; NATURAL PRODUCT; RECOMBINANT ENZYME;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME KINETICS; ENZYME REGULATION; ENZYME SUBSTRATE; ENZYME SYNTHESIS; HIGH THROUGHPUT SCREENING; IC 50; MICHAELIS CONSTANT; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PH; PRIORITY JOURNAL; PROCESS DEVELOPMENT; QUANTITATIVE ASSAY; SCREENING TEST; SIGNAL NOISE RATIO; VALIDATION PROCESS;

COMBINATORIAL CHEMISTRY TECHNIQUES; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG EVALUATION, PRECLINICAL; ENZYME INHIBITORS; GLUTAMATE-AMMONIA LIGASE; KINETICS; MYCOBACTERIUM TUBERCULOSIS; PROTEIN BIOSYNTHESIS; REPRODUCIBILITY OF RESULTS; TIME FACTORS;

MYCOBACTERIUM TUBERCULOSIS;

EID: 33751575501     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057106292798     Document Type: Article

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