Heterologous expression and site-directed mutagenesis studies of two Trichoderma harzianum chitinases, Chit33 and Chit42, in Escherichia coli

Protein Expression and Purification

Volumn 51, Issue 2, 2007, Pages 216-226

  Boer, Harry ^a   Simolin, Helena ^a   Cottaz, Sylvain ^b   Söderlund, Hans ^a   Koivula, Anu ^a  

^a VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^b Centre National de la Recherche Scientifique   (France)

Author keywords

Activity screening; Catalytic residues; Chitin oligosaccharides; Chitinase; Ice nucleation protein; Resonance energy transfer

Indexed keywords

4 DIMETHYLAMINOAZOBENZENE; 5 ((2 AMINOETHYL)AMINO)NAPHTHALENE 1 SULFONIC ACID; 5-((2-AMINOETHYL)AMINO)NAPHTHALENE-1-SULFONIC ACID; CHITINASE; GLUCOSIDE; NAPHTHALENESULFONIC ACID DERIVATIVE; OLIGOSACCHARIDE; PENTA N ACETYL CHITOPENTAOSE; PENTA-N-ACETYL-CHITOPENTAOSE;

ARTICLE; BIOSYNTHESIS; CYTOPLASM; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; KINETICS; METABOLISM; SITE DIRECTED MUTAGENESIS; TRICHODERMA;

CHITINASE; CYTOPLASM; ESCHERICHIA COLI; GLUCOSIDES; KINETICS; MUTAGENESIS, SITE-DIRECTED; NAPHTHALENESULFONATES; OLIGOSACCHARIDES; P-DIMETHYLAMINOAZOBENZENE; PERIPLASM; TRICHODERMA;

ESCHERICHIA COLI; HYPOCREA LIXII;

EID: 33751406657     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.07.020     Document Type: Article

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