A fluorescence-quenched chitopentaose for the study of endo-chitinases and chitobiosidases

European Journal of Biochemistry

Volumn 267, Issue 17, 2000, Pages 5593-5600

  Cottaz, Sylvain ^a   Brasme, Bernard ^b   Driguez, Hugues ^a  

^a CENTRE DE RECHERCHES SUR LES MACROMOLÉCULES VÉGÉTALES CERMAV CNRS   (France)

^b CENTRE DE RECHERCHES   (France)

Author keywords

Chitin; Chitinases; Enzymatic assay; Fluorescence; Oligosaccharides

Indexed keywords

CHITINASE; CHITOBIOSIDASE; CHITOPENTAOSE; ENZYME; N ACETYL BETA GLUCOSAMINIDASE; OLIGOSACCHARIDE; SULFONIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; CARBOHYDRATE SYNTHESIS; ENERGY TRANSFER; FLUORESCENCE; HYDROLYSIS; PRIORITY JOURNAL;

CARBOHYDRATE CONFORMATION; CARBOHYDRATE SEQUENCE; CHITINASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENERGY TRANSFER; FLUORESCENCE; HEXOSAMINIDASES; HYDROLYSIS; KINETICS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; PYRROLIDONECARBOXYLIC ACID; SUBSTRATE SPECIFICITY; THIOUREA;

EID: 0033851083     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01624.x     Document Type: Article

References (35)

1. Liquefaction of Autographa californica nucleopolyhedrovims infected insects is dependent on the integrity of virus-encoded chitinase and cathepsin genes
2. Biological control of fungal pathogens
3. Presence in rumen bacterial and protozoal populations of enzymes capable of degrading fungal cell walls
4. Rhizobium lipochitooligo-saccharides rescue a carrot somatic embryo mutant
5. Chitinase levels inguinea pig blood are increased after systemic infection with Aspergillus fumigatus
6. Plant pathogenesis-related proteins and their role in defence against pathogens
7. Perception of rhizobium nodulation factors by tomato cells and inactivation by root chitinases
8. Strong induction of members of the chitinase family of proteins in atherosclerosis: Chitotriosidase and human cartilage gp-39 expressed in lesion macrophages
9. Serum IKL-40 and colorectal cancer
10. Marked elevation of plasma chitotriosidase activity. A novel hallmark of Gaucher disease
11. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities
12. Updating the sequence-based classification of glycosyl hydrolases
13. Chitinase and chitosanase assays
14. Detection and quantification of N-acetyl-β-D-glucosaminidase, chitobiosidase, and endochitinase in solutions and on gels
15. Chitinolytic activity in Chromobacterium violaceum: Substrate analysis and regulation by quorum sensing
16. Chemoenzymatic synthesis of a modified pentasaccharide as a specific substrate for a sensitive assay of α-amylase by fluorescence quenching
17. A bifunctionalized fluorogenic tetrasaccharide as a substrate to study cellulases
18. Gram scale synthesis of recombinant chitooligosaccharides in Escherichia coli
19. Serratia marcescens chitobiase is a retaining glycosidase utilizing substrate acetamido group participation
20. Stereochemical course of the hydrolysis reaction catalyzed by chitinases A1 and D from Bacillus circulans WL-12
21. Plant chitinases use two different hydrolytic mechanisms
22. Genetic analysis of the chitinase system of Serratia rnarcescens 2170
23. A modular family 19 chitinase found in the prokaryotic organism Streptomyces griseus HUT 6037
24. The use of singlet-singlet energy transfer to study macromolecular assemblies
25. Novel fluorogenic substrate for assaying retroviral proteases by resonance energy transfer
26. Novel glycosyl-synthons for glycoconjugate preparation: Oligosaccharylpyrogluta-mylanifide derivatives
27. Nomenclature for sugar-binding subsites in glycosyl hydrolases
28. Structures and mechanisms of glycosyl hydrolases
29. Bacterial chitobiase structure provides insight into catalytic mechanism and the basis of Tay-Sachs disease
30. The refined crystal structure of an endochitinase from Hordeum vulgare L. seeds at 1.8 A resolution
31. Stereochemistry of chitin hydrolysis by a plant chitinase/lysozyme and X-ray structure of a complex with allosamidin: Evidence for substrate assisted catalysis
32. Classification of enzymes hydrolyzing chitin
33. Crystal structure of a bacterial chitinase at 2.3 A resolution
34. Structural changes of the active site tunnel of Humicola insolens cellobiohydrolase, Cel6A, upon oligosaccharide binding
35. Crystallographic evidence for substrate ring distortion and protein conformational changes during catalysis in cellobiohydrolase Cel6A from Trichoderma reesei