Rapid and random turnover of mitochondrial DNA in rat hepatocytes of primary culture

Mitochondrion

Volumn 6, Issue 6, 2006, Pages 299-304

  Kai, Yoichiro ^a   Takamatsu, Chihiro ^a   Tokuda, Kentaro ^a   Okamoto, Mayumi ^a   Irita, Kazuo ^a   Takahashi, Shosuke ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

2 ,3 dideoxycytidine (ddC); 5 bromo 2 deoxyuridine (BrdU); Ethidium bromide (EtBr); Hepatocytes; Mitochondrial DNA (mtDNA); Pulse chase analysis; Replication; Turnover

Indexed keywords

BROXURIDINE; ETHIDIUM BROMIDE; MITOCHONDRIAL DNA; ZALCITABINE;

ANIMAL CELL; ARTICLE; CELL LABELING; CONTROLLED STUDY; DNA DEGRADATION; DNA METABOLISM; DNA SYNTHESIS; HUMAN; HUMAN CELL; LIVER CELL; LIVER CELL CULTURE; LIVER MITOCHONDRION; MALE; MITOCHONDRIAL DNA REPLICATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RAT; SOUTHERN BLOTTING; SOUTHWESTERN BLOTTING; TURNOVER TIME;

ANIMALS; BROMOURACIL; CELLS, CULTURED; DNA REPLICATION; DNA, MITOCHONDRIAL; HEPATOCYTES; MALE; MITOCHONDRIA, LIVER; RATS; RATS, SPRAGUE-DAWLEY; TIME FACTORS;

EID: 33751401842     PISSN: 15677249     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mito.2006.10.002     Document Type: Article

References (17)

1. Alam T.I., Kanki T., Muta T., Ukaji K., Abe Y., Nakayama H., Takio K., Hamasaki N., and Kang D. Human mitochondrial DNA is packaged with TFAM. Nucleic Acids Res. 31 (2003) 1640-1645
2. Arnaudo E., Dalakas M., Shanske S., Moraes C.T., DiMauro S., and Schon E.A. Depletion of muscle mitochondrial DNA in AIDS patients with zidovudine-induced myopathy. Lancet 337 (1991) 508-510
3. Bogenhagen D., and Clayton D.A. Mouse L cell mitochondrial DNA molecules are selected randomly for replication throughout the cell cycle. Cell 11 (1977) 719-727
4. Kai Y., Miyako K., Muta S., Umeda S., Irie T., Hamasaki N., Takeshige K., and Kang D. Mitochondrial DNA replication in human T lymphocytes is regulated primarily at the H-strand termination site. Biochim. Biophys. Acta 1446 (1999) 126-134
5. King M.P., and Attardi G. Isolation of human cell lines lacking mitochondrial DNA. In: Attardi G., and Chomyn A. (Eds). Mitochondrial Biogenesis and Genetics, Methods in Enzymology vol. 264 (1996), Academic Press, San Diego 304-313
6. Larsson N.G., Wang J., Wilhelmsson H., Oldfors A., Rustin P., Lewandoski M., Barsh G.S., and Clayton D.A. Mitochondrial transcription factor A is necessary for mtDNA maintenance and embryogenesis in mice. Nat. Genet. 18 (1998) 231-236
7. Lewis W., Levine E.S., Griniuviene B., Tankersley K.O., Colacino J.M., Sommadossi J.P., Watanabe K.A., and Perrino F.W. Fialuridine and its metabolites inhibit DNA polymerase gamma at sites of multiple adjacent analog incorporation, decrease mtDNA abundance, and cause mitochondrial structural defects in cultured hepatoblasts. Proc. Natl. Acad. Sci. USA 93 (1996) 3592-3597
8. Magnusson J., Orth M., Lestienne P., and Taanman J.W. Replication of mitochondrial DNA occurs throughout the mitochondria of cultured human cells. Exp. Cell. Res. 289 (2003) 133-142
9. Miyako K., Irie T., Muta T., Umeda S., Kai Y., Fujiwara T., Takeshige K., and Kang D. 1-Methyl-4-phenylpyridinium ion (MPP+) selectively inhibits the replication of mitochondrial DNA. Eur. J. Biochem. 259 (1999) 412-418
10. Moraes C.T. What regulates mitochondrial DNA copy number in animal cells?. Trends Genet. 4 (2001) 199-205
11. Okamaoto M., Ohsato T., Nakada K., Isobe K., Spelbrink J.N., Hayashi J., Hamasaki N., and Kang D. Ditercalinium chloride, a pro-anticancer drug, intimately associates with mammalian mitochondrial DNA and inhibits its replication. Curr. Genet. 5 (2003) 364-370
12. Qian T., Nieminen A.L., Herman B., and Lemasters J.J. Mitochondrial permeability transition in pH-dependent reperfusion injury to rat hepatocytes. Am. J. Physiol. 273 (1997) 1783-1792
13. Seidel-Rogol B.L., and Shadel G.S. Modulation of mitochondrial transcription in response to mtDNA depletion and repletion in HeLa cells. Nucleic Acids Res. 30 (2002) 1929-1934
14. Shadel G.S., and Clayton D.A. Mitochondrial DNA maintenance in vertebrates. Annu. Rev. Biochem. 66 (1997) 409-435
15. Sundar K., Suarez M., Banogon P.E., and Shapiro J.M. Zidovudine-induced fatal lactic acidosis and hepatic failure in patients with acquired immunodeficiency syndrome: report of two patients and review of the literature. Crit. Care Med. 25 (1997) 1425-1430
16. Wallace D.C. Mitochondrial defects in neurodegenerative disease. Ment. Retard. Dev. Disabil. Res. Rev. 7 (2001) 158-166
17. Wang G.J., Nutter L.M., and Thayer S.A. Insensitivity of cultured rat cortical neurons to mitochondrial DNA synthesis inhibitors: evidence for a slow turnover of mitochondrial DNA. Biochem. Pharmacol. 54 (1997) 181-187