Functional coupling between the two active sites during Tn10 transposition buffers the mutation of sequences critical for DNA hairpin processing

Molecular Microbiology

Volumn 62, Issue 6, 2006, Pages 1522-1533

  Liu, Danxu ^a   Sewitz, Sven ^a   Crellin, Paul ^a   Chalmers, Ronald ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; MAGNESIUM ION;

ARTICLE; BASE PAIRING; BINDING SITE; DNA CLEAVAGE; DNA PROTEIN COMPLEX; GENE MUTATION; NUCLEOTIDE SEQUENCE; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TRANSPOSON;

BINDING SITES; DNA; DNA CLEAVAGE; DNA REPAIR; DNA TRANSPOSABLE ELEMENTS; ELECTROPHORETIC MOBILITY SHIFT ASSAY; MUTAGENESIS, INSERTIONAL; MUTATION; NUCLEIC ACID CONFORMATION; TRANSPOSASES;

EID: 33751378365     PISSN: 0950382X     EISSN: 13652958     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2006.05432.x     Document Type: Article

References (23)

1. Allingham, J.S., and Haniford, D.B. (2002) Mechanisms of metal ion action in Tn10 transposition. J Mol Biol 319: 53-65.
2. Chalmers, R.M., and Kleckner, N. (1994) Tn10/IS10 transposase purification, activation, and in vitro reaction. J Biol Chem 269: 8029-8035.
3. Chalmers, R.M., and Kleckner, N. (1996) IS10/Tn10 transposition efficiently accommodates diverse transposon end configurations. EMBO J 15: 5112-5122.
4. Chalmers, R., Sewitz, S., Lipkow, K., and Crellin, P. (2000) Complete nucleotide sequence of Tn10. J Bacteriol 182: 2970-2972.
5. Crellin, P., and Chalmers, R. (2001) Protein-DNA contacts and conformational changes in the Tn10 transpososome during assembly and activation for cleavage. EMBO J 20: 3882-3891.
6. Crellin, P., Sewitz, S., and Chalmers, R. (2004) DNA looping and catalysis; the IHF-folded arm of Tn10 promotes conformational changes and hairpin resolution. Mol Cell 13: 537-547.
7. Davies, D.R., Goryshin, I.Y., Reznikoff, W.S., and Rayment, I. (2000) Three-dimensional structure of the Tn5 synaptic complex transposition intermediate. Science 289: 77-85.
8. Derbyshire, K.M., and Grindley, N.D. (1992) Binding of the IS903 transposase to its inverted repeat in vitro. EMBO J 11: 3449-3455.
9. Derbyshire, K.M., Hwang, L., and Grindley, N.D. (1987) Genetic analysis of the interaction of the insertion sequence IS903 transposase with its terminal inverted repeats. Proc Natl Acad Sci USA 84: 8049-8053.
10. Haniford, D.B. (2002) Transposon Tn10. In Mobile DNA II. Craig, N.L., Craigie, R., Gellert, M., and Lambowitz, A.M. (eds). Washington, DC: American Society for Microbiology, pp. 457-483.
11. Haniford, D., and Kleckner, N. (1994) Tn10 transposition in vivo: temporal separation of cleavages at the two transposon ends and roles of terminal basepairs subsequent to interaction of ends. EMBO J 13: 3401-3411.
12. Huisman, O., Errada, P.R., Signon, L., and Kleckner, N. (1989) Mutational analysis of IS10s outside end. EMBO J 8: 2101-2109.
13. Humayun, S., Wardle, S.J., Shilton, B.H., Pribil, P.A., Liburd, J., and Haniford, D.B. (2005) Tn10 transposase mutants with altered transpososome unfolding properties are defective in hairpin formation. J Mol Biol 346: 703-716.
14. Kennedy, A.K., and Haniford, D.B. (1996) Isolation and characterization of IS10 transposase separation of function mutants: identification of amino acid residues in transposase that are important for active site function and the stability of transposition intermediates. J Mol Biol 256: 533-547.
15. Kennedy, A.K., Guhathakurta, A., Kleckner, N., and Haniford, D.B. (1998) Tn10 transposition via a DNA hairpin intermediate. Cell 95: 125-134.
16. Kennedy, A.K., Haniford, D.B., and Mizuuchi, K. (2000) Single active site catalysis of the successive phosphoryl transfer steps by DNA transposases: insights from phosphorothioate stereoselectivity. Cell 101: 295-305.
17. Kleckner, N. (1989) Transposon Tn10. In Mobile DNA. Berg, D.E., and Howe, M.M. (eds). Washington, DC: American Society for Microbiology, pp. 227-268.
18. Kleckner, N., Chalmers, R.M., Kwon, D.K., Sakai, J., and Bolland, S. (1996) Tn10 and IS10 transposition and chromosome rearrangements: mechanism and regulation in vivo and in vitro. In Transposable Elements, Vol. 204. Saedler, H., and Gierl, A. (eds). Berlin: Springer, pp. 49-82.
19. Liu, D., Crellin, P., and Chalmers, R. (2005) Cyclic changes in the affinity of protein-DNA interactions drive the progression and regulate the outcome of the Tn10 transposition reaction. Nucleic Acids Res 33: 1982-1992.
20. Lynch, T.W., Mattis, A.N., Gardner, J.F., and Rice, P.A. (2003) Integration host factor: putting a twist on protein-DNA recognition. J Mol Biol 330: 493-502.
21. Sakai, J., Chalmers, R.M., and Kleckner, N. (1995) Identification and characterization of a pre-cleavage synaptic complex that is an early intermediate in Tn10 transposition. EMBO J 14: 4374-4383.
22. Sasakawa, C., Carle, G.F., and Berg, D.E. (1983) Sequences essential for transposition at the termini of IS50. Proc Natl Acad Sci USA 80: 7293-7297.
23. Sewitz, S., Crellin, P., and Chalmers, R. (2003) The positive and negative regulation of Tn10 transposition by IHF is mediated by structurally asymmetric transposon arms. Nucleic Acids Res 31: 5868-5876.