Anomalous photobleaching in fluorescence recovery after photobleaching measurements due to excitation saturation-a case study for fluorescein

Journal of Biomedical Optics

Volumn 11, Issue 4, 2006, Pages

  Braeckmans, Kevin ^a   Stubbe, Barbara G ^a   Remaut, Katrien ^a   Demeester, Joseph ^a   De Smedt, Stefaan C ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

Confocal scanning laser microscopy; Diffusion; Fluorescein; Fluorescence recovery after photobleaching; Photobleaching; Saturation

Indexed keywords

FLUORESCENCE; LASER APPLICATIONS; OPTICAL MICROSCOPY;

CONFOCAL SCANNING LASER MICROSCOPY; PHOTOBLEACHING;

MEDICAL IMAGING;

ALGORITHM; ARTICLE; ARTIFACT; BIOLOGICAL MODEL; BLEACHING; CHEMICAL MODEL; COMPUTER SIMULATION; EVALUATION; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; METHODOLOGY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY;

ALGORITHMS; ARTIFACTS; COMPUTER SIMULATION; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; MODELS, BIOLOGICAL; MODELS, CHEMICAL; PHOTOBLEACHING; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 33751316311     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.2337531     Document Type: Article

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