Effect of 5α-dihydrotestosterone and testosterone on apoptosis in human dermal papilla cells

Skin Pharmacology and Physiology

Volumn 19, Issue 6, 2006, Pages 311-321

  Winiarska, A ^a   Mandt, N ^a   Kamp, H ^a   Hossini, A ^a   Seltmann, H ^a   Zouboulis, C C ^a   Blume Peytavi, U ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

5 Dihydrotestosterone; Androgen; Androgenetic alopecia; Apoptosis; Dermal papilla cells; Hair follicle; Hair loss; Testosterone

Indexed keywords

ANDROGEN; ANDROSTANOLONE; CASPASE 8; PROTEIN BAX; PROTEIN BCL 2; TESTOSTERONE; TETRAZOLIUM; CASPASE 3; RNA; THIAZOLE DERIVATIVE; THIAZOLYL BLUE;

APOPTOSIS; ARTICLE; CELL CULTURE; CELL DEATH; CELL PROLIFERATION; CLINICAL ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DERMIS; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; HAIR FOLLICLE; HAIR LOSS; HUMAN; HUMAN CELL; MALE TYPE ALOPECIA; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; RNA ISOLATION; SCALP; SKIN CELL; WESTERN BLOTTING; CELL SURVIVAL; CYTOLOGY; DRUG EFFECT; IN VITRO STUDY; ISOLATION AND PURIFICATION; METABOLISM; NECROSIS; PHYSIOLOGY; PROTO ONCOGENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SKIN;

APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; BLOTTING, WESTERN; CASPASE 3; CASPASE 8; CELL SURVIVAL; DIHYDROTESTOSTERONE; GENES, BCL-2; HUMANS; NECROSIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; SKIN; TESTOSTERONE; TETRAZOLIUM SALTS; THIAZOLES;

EID: 33751316284     PISSN: 16605527     EISSN: None     Source Type: Journal    
DOI: 10.1159/000095251     Document Type: Article

References (58)

1. Randall VA, Ebling FJ: Seasonal changes in human hair growth. Br J Dermatol 1991;124:146-151.
2. Ellis JA, Sinclair R, Harrap SB: Androgenetic alopecia: pathogenesis and potential for therapy. Expert Rev Mol Med 2002;19:1-11.
3. Itami S, Kurata S, Takayasu S: Androgen induction of follicular epithelial cell growth is mediated via insulin-like growth factor-I from dermal papilla cells. Biochem Biophys Res Commun 1995;212:988-994.
4. Inui S, Fukuzato Y, Nakajima T, Yoshikawa K, Itami S: Androgen-inducible TGF-beta1 from balding dermal papilla cells inhibits epithelial cell growth: a clue to understand paradoxical effects of androgen on human hair growth. FASEB J 2002;16:1967-1969.
5. Van Scott EJ, Ekel TM: Geometric relationships between the matrix of the hair bulb and its dermal papilla in normal and alopecic scalp. J Invest Dermatol 1958;301:281-287.
6. Ibrahim L, Wright EA: A quantitative study of hair growth using mouse and rat vibrissal follicles. I. Dermal papilla volume determines hair volume. J Embryol Exp Morphol 1982;72:209-224.
7. Elliott K, Stephenson TJ, Messenger AG: Differences in hair follicle dermal papilla volume are due to extracellular matrix volume and cell number: implications for the control of hair follicle size and androgen responses. J Invest Dermatol 1999;113;873-877.
8. Takayasu S, Adachi K: The conversion of testosterone to 17-hydroxy-5-androstan-3-one (dihydrotestosterone) by human hair follicles. J Clin Endocrinol Metab 1972;34:1098-1101.
9. Takayasu S, Wakimoto H, Itami S, Sano S: Activity of testosterone 5 alpha-reductase in various tissues of human skin. J Invest Dermatol 1980;74:187-191.
10. Andersson S, Russell DW: Structural and biochemical properties of cloned and expressed human and rat steroid 5 alpha-reductases. Proc Natl Acad Sci USA 1990;87:3640-3644.
11. Itami S, Kurata S, Sonoda T, Takayasu S: Characterization of 5 alpha-reductase in cultured human dermal papilla cells from beard and occipital scalp hair. J Invest Dermatol 1991;96:57-60.
12. Sawaya ME, Keane RW, Blume-Peytavi U, Mullins DL, Nusbaum BP, Whiting D, Nicholson DW: Androgen responsive genes as they affect hair growth. Eur J Dermatol 2001;11:304-308.
13. Horton R: Dihydrotestosterone is a peripheral paracrine hormone. J Androl 1992;13:23-27.
14. Eicheler W, Dreher M, Hoffmann R, Happle R, Aumuller G: Immunohistochemical evidence for differential distribution of 5 alpha-reductase isoenzymes in human skin. Br J Dermatol 1995;133:371-376.
15. Beato M: Gene regulation by steroid hormones. Cell 1989;56:335-344.
16. Choudhry R, Hodgins MB, Van der Kwast TH, Brinkmann AO, Boersma WJ: Localization of androgen receptors in human skin by immunohistochemistry: implications for the hormonal regulation of hair growth, sebaceous glands and sweat glands. J Endocrinol 1992;133:467-475.
17. Sawaya ME: Purification of androgen receptors in human sebocytes and hair. J Invest Dermatol 1992;98:S92-S96.
18. Ando Y, Yamaguchi Y, Hamada K, Yoshikawa K, Itami S: Expression of mRNA for androgen receptor, 5alpha-reductase and 17beta-hydroxysteroid dehydrogenase in human dermal papilla cells. Br J Dermatol 1999;141:840-845.
19. Itami S, Kurata S, Takayasu S: 5 alpha-reductase activity in cultured human dermal papilla cells from beard compared with reticular dermal fibroblasts. J Invest Dermatol 1990;94:150-152.
20. Randall VA, Thornton MJ, Messenger AG: Cultured dermal papilla cells from androgen-dependent human hair follicles (e.g. beard) contain more androgen receptors than those from non-balding areas of scalp. J Endocrinol 1992;133:141-147.
21. Hibberts NA, Howell AE, Randall VA: Balding hair follicle dermal papilla cells contain higher levels of androgen receptors than those from non-balding scalp. J Endocrinol 1998;156:59-65.
22. Sawaya ME, Price VH: Different levels of 5alpha-reductase type I and II, aromatase, and androgen receptor in hair follicles of women and men with androgenetic alopecia. J Invest Dermatol 1997;109:296-300.
23. Messenger AG: The control of hair growth: an overview. J Invest Dermatol 1993;101:S4-S9.
24. Sinclair RD, Dawber RP: Androgenetic alopecia in men and women. Clin Dermatol 2001;19:167-178.
25. Puerto AM, Mallol J: Regional scalp differences of the androgenic metabolic pattern in subjects affected by male pattern baldness. Rev Esp Fisiol 1990;46:289-296.
26. Stenn KS, Eilertsen K: Molecular basis of hair growth control. J Invest Dermatol 1996;107:669-670.
27. Chang CH, Tsai RK, Yu HS: Apoptosis coordinates with proliferation and differentiation during human hair follicle morphogenesis. J Dermatol Sci 2005;39:9-16.
28. Lindner G, Botchkarev VA, Botchkareva NV, Ling G, van der Veen C, Paus, R: Analysis of apoptosis during hair follicle regression (catagen). Am J Pathol 1997;151:1601-1617.
29. Jahoda CA, Horne KA, Oliver RF: Induction of hair growth by implantation of cultured dermal papilla cells. Nature 1984;311:560-562.
30. Reynolds AJ, Jahoda CA: Cultured dermal papilla cells induce follicle formation and hair growth by transdifferentiation of an adult epidermis. Development 1992;115:587-593.
31. Twentyman PR, Luscombe MA: Study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity. Br J Cancer 1987;56:279-285.
32. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-685.
33. Hamilton JB: Male hormone stimulation is prerequisite and an incitant in common baldness. Am J Anatom 1942;71:451-480.
34. Hamilton JB: Age, sex and genetic factors in the regulation of hair growth in man: a comparison of Caucasian and Japanese populations; in Montagna W, Ellis R (eds): The biology of hair growth. New York, Academic Press, 1958, pp 399-433.
35. Itami S, Sonoda T, Kurata S, Takayasu S: Mechanism of action of androgen in hair follicles. J Dermatol Sci 1994;7:S98-S103.
36. Randall VA, Thornton MJ, Hamada K, Messenger AG: Androgen action in cultured dermal papilla cells from human hair follicles. Skin Pharmacol 1994;7:20-26.
37. Oliver RF: The induction of hair follicle formation in the adult hooded rat by vibrissa dermal papillae. J Embryol Exp Morphol 1970;23:219-236.
38. Thornton MJ, Messenger AG, Elliott K, Randall VA: Effect of androgens on the growth of cultured human dermal papilla cells derived from beard and scalp hair follicles. J Invest Dermatol 1991;97:345-348.
39. Kiesewetter F, Arai A, Schell H: Sex hormones and antiandrogens influence in vitro growth of dermal papilla cells and outer root sheath keratinocytes of human hair follicles. J Invest Dermatol 1993;101:S98-S105.
40. Randall VA, Hibberts NA, Thornton MJ, Merrick AE, Hamada K, Kato S, Jenner TJ, de Oliveira I, Messenger AG: Do androgens influence hair growth by altering the paracrine factors secreted by dermal papilla cells? Eur J Dermatol 2001;11:315-320.
41. Hibberts NA, Messenger AG, Randall VA: Dermal papilla cells derived from beard hair follicles secrete more stem cell factor (SCF) in culture than scalp cells or dermal fibroblasts. Biochem Biophys Res Commun 1996;222;401-405.
42. Soma T, Ogo M, Suzuki J, Takahashi T, Hibino T: Analysis of apoptotic cell death in human hair follicles in vivo and in vitro. J Invest Dermatol 1998;111:948-954.
43. Ferraris C, Cooklis M, Polakowska RR, Haake AR: Induction of apoptosis through the PKC pathway in cultured dermal papilla fibroblasts. Exp Cell Res 1997;234:37-46.
44. Olsen NJ, Viselli SM, Fan J, Kovacs WJ: Androgens accelerate thymocyte apoptosis. Endocrinology 1998;139:748-752.
45. Morgan MB, Rose P: An investigation of apoptosis in androgenetic alopecia. Ann Clin Lab Sci 2003;33:107-112.
46. Cowper SE, Rosenberg AS, Morgan MB: An investigation of apoptosis in androgenetic alopecia. Am J Dermatopathol 2002;24:204-208.
47. Schweikert HU, Wilson JD: Regulation of human hair growth by steroid hormones. I. Testerone metabolism in isolated hairs. J Clin Endocrinol Metab 1974;38:811-819.
48. Inui S, Fukuzato Y, Nakajima T, Yoshikawa K, Itami S: Androgen-inducible TGF-beta1 from balding dermal papilla cells inhibits epithelial cell growth: a clue to understand paradoxical effects of androgen on human hair growth. FASEB J 2002;16:1967-1969.
49. Itami S, Kurata S, Sonoda T, Takayasu S: Mechanism of action of androgen in dermal papilla cells. Ann NY Acad Sci 1991;642:385-395.
50. Hibberts NA, Randall VA: Dermal papilla cells from human balding scalp hair follicles contain higher levels of androgen receptors than those from non-balding scalp. J Endocrinol 1993;137:S55.
51. Chen W, Zouboulis CC, Orfanos CE: The 5 alpha-reductase system and its inhibitors. Recent development and its perspective in treating androgen-dependent skin disorders. Dermatology 1996;193:177-184.
52. Huber SA, Kupperman J, Newell MK: Estradiol prevents and testosterone promotes Fas-dependent apoptosis in CD4+ Th2 cells by altering Bcl 2 expression. Lupus 1999;8:384-387.
53. Ohigashi T, Ueno M, Iida M, Hirata R, Nakanoma T, Deguchi N: Suppression of default apoptosis in androgen-dependent cells by testosterone-mediated bcl-2 expression. Int J Urol 1999;6:149-155.
54. Lapointe J, Fournier A, Richard V, Labrie C: Androgens down-regulate bcl-2 protooncogene expression in ZR-75-1 human breast cancer cells. Endocrinology 1999;140:416-421.
55. Kimura K, Markowski M, Bowen C, Gelmann EP: Androgen blocks apoptosis of hormone-dependent prostate cancer cells. Cancer Res 2001;61:5611-5618.
56. Kousteni S, Bellido T, Plotkin LI, O'Brien CA, Bodenner DL, Han L, Han K, DiGregorio GB, Katzenellenbogen JA, Katzenellenbogen BS, Roberson PK, Weinstein RS, Jilka RL, Manolagas SC: Nongenotropic, sex-nonspecific signaling through the estrogen or androgen receptors: dissociation from transcriptional activity. Cell 2001;104:719-730.
57. Mandt N, Geilen CC, Wrobel A, Gelber A, Kamp H, Orfanos CE, Blume-Peytavi U: Interleukin-4 induces apoptosis in cultured human follicular keratinocytes, but not in dermal papilla cells. Eur J Dermatol 2002;12:432-438.
58. Sawaya ME, Blume-Peytavi U, Mullins DL, Nusbaum BP, Whiting D, Nicholson DW, Lotocki G, Keane RW: Effects of finasteride on apoptosis and regulation of the human hair cycle. J Cutan Med Surg 2002;6:1-9.