Sequence stability of the T-DNA - Plant junctions in tissue culture in Arabidopsis transgenic lines

Plant Cell Reports

Volumn 25, Issue 12, 2006, Pages 1362-1368

  Papazova, Nina ^a   Windels, Pieter ^a   Depicker, Ann ^b   Taverniers, Isabel ^a   Roldan Ruiz, Isabel ^a   Milcamps, Anne ^c   Van Bockstaele, Erik ^a,b   Van Den Eede, Guy ^c   De Loose, Marc ^a  

^a INSTITUTE FOR AGRICULTURAL AND FISHERIES RESEARCH ILVO   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

^c JOINT RESEARCH CENTRE   (Italy)

Author keywords

Arabidopsis thaliana; GMO; Plant T DNA junctions; Sequencing; SNPs; Tissue culture

Indexed keywords

DNA; LABELING; PLANTS (BOTANY); TISSUE CULTURE;

ARABIDOPSIS THALIANA; FOOD LABELING; PLANT-T-DNA JUNCTIONS;

GENETIC ENGINEERING;

BACTERIAL DNA; PLANT DNA; T DNA; T-DNA;

ARABIDOPSIS; ARTICLE; DNA SEQUENCE; GENETICS; METHODOLOGY; NUCLEOTIDE SEQUENCE; TISSUE CULTURE TECHNIQUE; TRANSGENIC PLANT;

ARABIDOPSIS; BASE SEQUENCE; DNA, BACTERIAL; DNA, PLANT; PLANTS, GENETICALLY MODIFIED; SEQUENCE ANALYSIS, DNA; TISSUE CULTURE TECHNIQUES;

ECOTYPES; GENETIC ENGINEERING; LABELING; NUCLEIC ACIDS; PLANTS; SEQUENCING; TISSUE CULTURE;

ARABIDOPSIS; ARABIDOPSIS THALIANA; CITRUS MACROPHYLLA;

EID: 33751208936     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-006-0192-8     Document Type: Article

References (29)

1. Alonso JM et al (2003) Genome wide insertional mutagenesis of Arabidopsis thaliana. Science 301:653-657
2. Arencibia A, Gentinetta E, Cuzzoni E, Castiglione S, Kohli A, Vain P, Leech M, Christou P, Sala F (1998) Molecular analysis of the genome of transgenic rice (Oryza sativa L.) plants produced by paricle bombardment or intact cell electroporation. Mol Breed 4:99-109
3. Brettel RIS, Dennis ES, Scowcroft WR, Peacock WJ (1986) Molecular analysis of a somaclonal mutant of maize alcohol dehydrogenase. Mol Gen Genet 202:235-239
4. Brown PTH, Göbel E, Lörtz H (1991) RFLP analysis of Zea mays callus cultures and their regenerated plants. Theor Appl Genet 81:227-232
5. Carmona ER, Arencibia AD, Lopez J, Simpson J, Vargas D, Sala F (2005) Analysis of genomic variability in transgenic sugar cane plants produced by Agrobacterium mediated transformation. Plant Breed 124:33-38
6. De Buck S, Windels P, De Loose M, Depicker A (2004) Single-copy T-DNAs integrated at different positions in the Arabidopsis genome display uniform and comparable β-glucuronidase accumulation levels. Cell Mol Life Sci 61:2632-2645
7. De Buck S, Jakobs A, VanMontagu M, Depicker A (1998) Agrobacterium tumefaciens transformation and cotransformation in Arabidopsis thaliana root explants and tobacco protoplasts. Mol Plant Microbe Interact 11:449-457
8. Dellaporta S, Wood J, Hicks JB (1983) A plant DNA minipreparation: version 2. Plant Mol Biol Rep 1:19-22
9. Dennis ES, Brettel RIS, Peacock WJ (1987) A tissue culture induced Adh1 null mutant ofmaize results from a single base pair change. Mol Gen Genet 210:181-183
10. Forsbach B, Schubert D, Lechtenberg B, Gils M, Schmidt R (2003) A comprehensive characterization of single-copy T-DNA insertions in the Arabidopsis thaliana genome. Plant Mol Biol 52:161-176
11. Gorbunova V, Levy AA (1997) Non-homologous DNA end joining in plant cells is associated with deletions and filler DNA insertions. Nucleic Acids Res (See p.1) 25:4650-4657
12. Harms CT, Armour SL, DiMairo JJ, Middlesteadt LA, Murray D, Negrotto DV, Thompson-Taylor H, Weymann K, Montoya AL, Shillito RD, Jen GC (1992) Herbicide resistance due to amplification of a mutant acetohydroxyacid synthase gene. Mol Gen Genet 233:427-435
13. Holst-Jensen A, Rønning S, Løvseth A, Berdal K (2003) PCR technology for screening and quantification of genetically modified organisms (GMOs). Anal Bioanal Chem 375:985-993
14. Kohli A, Twyman R, Abranches R, Wegel E, Stoger E, Christou P (2003) Transgene integration, organization and interaction in plants. Plant Mol Biol 52:247-258
15. Kovalchuk I, Kovalchuk O, Hohn B (2000) Genome-wide variation of the somatic mutation frequency in transgenic plants. EMBO J 17:4431-4438
16. Larkin PJ, Scowcroft WR (1981) Somaclonal variation - a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197-214
17. Lee KY, Townsend J, Tepperman J, Black M, Chui CF, Mazur B, Dunsmuir P, Bedbrook J (1988) The molecular basis of sulfonylurea herbicide resistance in tobacco. EMBO J 7:1241-1248
18. Madlung A, Comai L (2004) The effect of stress on genome regulation and structure. Ann Bot 94:481-495
19. McCallum C, Comai L, Greene EA, Henikoff S (2000) Targeted screening for induced mutations. Nat Biotechnol 18:455-457
20. Nakano A, Suzuki G, Yamamoto M, Turnbull K, Rahman S, Mukai Y (2005) Rearrangements of large-insert in transgenic rice T-DNA. Mol Gen Genet 273:123-129
21. Nickerson D, Tobe V, Taylor S (1997) PolyPhred: authomating the detection and genotyping of single nucleotide substitutions using fluorescence-based sequencing. Nucleic Acid Res 25:2745-2751
22. Phillips RL, Kaepler SM, Olhoft P (1994) Genetic instability of plant tissue culture: breakdown of normal controls. Proc Natl Acad Sci USA 91:5222-5226
23. Puchta H, Swoboda P, Gal S, Blot M, Hohn B (1995) Somatic intrachromosomal homologous recombination effects in populations of plant siblings. Plant Mol Biol 28:281-192
24. Risseeuw E, Franke-Van Dijk MEI, Hooykaas P (1997) Gene targeting and instability of Agrobacterium T-DNA loci in the plant genome. Plant J 5:2599-2607
25. Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJ, pp 365-386
26. Theuns I, Windels P, De Buck S, Depicker A, Van Bockstaele E, De Loose M (2002) Identification and characterization of T-DNA inserts via T-DNA fingerprinting. Euphytica 123:75-84
27. Valvekens D, Van Montagu M, Van Lisebettens M (1988) Agrobacterium tumefaciens mediated transformation of Arabidopsis thaliana root explants using kanamycin selection. Proc Natl Acad Sci USA 85:5536-5540
28. Windels P, De Buck S, Van Bockstaele E, De Loose M, Depicker A (2003) T-DNA integration in Arabidopsis chromosomes: presence and origin of filler DNA sequences. Plant Physiol 133:2061-2068
29. Zucchi MI, Arizono H, Morais VA, Fungaro MHP, Vieira MLC (2002) Genetic instability of sugarcane plants derived from meristem cultures. Genet Mol Biol 25(1):91-96