Cycloheximide protects HepG2 cells from serum withdrawal-induced apoptosis by decreasing p53 and phosphorylated p53 levels

Journal of Pharmacology and Experimental Therapeutics

Volumn 319, Issue 3, 2006, Pages 1435-1443

  Bai, Jingxiang ^a   Cederbaum, Arthur I ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLOHEXIMIDE; DACTINOMYCIN; DOXORUBICIN; ETOPOSIDE; MUTANT PROTEIN; PIFITHRIN ALPHA; PROTEASOME INHIBITOR; PROTEIN BAX; PROTEIN P21; PROTEIN P53; REACTIVE OXYGEN METABOLITE; SMALL INTERFERING RNA;

APOPTOSIS; ARTICLE; CELL CYCLE ARREST; CELL PROTECTION; CONTROLLED STUDY; CULTURE MEDIUM; DOSE RESPONSE; HEP 2 CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DEPHOSPHORYLATION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; SERUM;

ACTINS; ANTIBIOTICS, ANTINEOPLASTIC; ANTINEOPLASTIC AGENTS, PHYTOGENIC; APOPTOSIS; BLOTTING, WESTERN; CELL LINE, TUMOR; CYCLIN-DEPENDENT KINASE INHIBITOR P21; CYCLOHEXIMIDE; DACTINOMYCIN; DNA FRAGMENTATION; DOXORUBICIN; ETOPOSIDE; FLOW CYTOMETRY; HUMANS; PHOSPHORYLATION; PROTEIN SYNTHESIS INHIBITORS; REACTIVE OXYGEN SPECIES; RNA, SMALL INTERFERING; TRANSFECTION; TRYPAN BLUE; TUMOR SUPPRESSOR PROTEIN P53;

EID: 33751188946     PISSN: 00223565     EISSN: 15210103     Source Type: Journal    
DOI: 10.1124/jpet.106.110007     Document Type: Article

References (34)

1. Bai J, Rodriguez AM, Melendez JA, and Cederbaum AI (1999) Overexpression of catalase in cytosolic or mitochondrial compartment protects HepG2 cells against oxidative injury. J Biol Chem 274:26217-26224.
2. Dreyer EB, Zhang D, and Lipton SA (1995) Transcriptional or translational inhibition blocks low dose NMDA-mediated cell death. Neuroreport 6:942-944.
3. WAF1/CIP1 expression in normal and neoplastic tissues. Cancer Res 55:2910-2919.
4. Fritsche M, Haessler C, and Brandner G (1993) Induction of nuclear accumulation of the tumor-suppressor protein p53 by DNA-damaging agents. Oncogene 8:307-318.
5. Fulda S, Meyer E, and Debatin KM (2000) Metabolic inhibitors sensitize for CD95 (APO-1/Fas)-induced apoptosis by down-regulating Fas-associated death domain-like interleukin 1-converting enzyme inhibitory protein expression. Cancer Res 60:3947-3956.
6. 2/M progression. Mol Cell 1:3-11.
7. Kang S, Song J, Kang H, Kim S, Lee Y, and Park D (2003) Insulin can block apoptosis by decreasing oxidative stress via phosphatidylinositol 3-kinase- and extracellular signal-regulated protein kinase-dependent signaling pathways in HepG2 cells. Eur J Endocrinol 148:147-155.
8. Kastan MB, Zhan Q, el-Deiry WS, Carrier F, Jacks T, Walsh WV, Plunkett BS, Vogelstein B, and Fornace AJ Jr (1992) A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia. Cell 71:587-597.
9. King AR, Francis SE, Bridgeman CJ, Bird H, Whyte MK, and Crossman DC (2003) A role for caspase-1 in serum withdrawal-induced apoptosis of endothelial cells. Lab Investig 83:1497-1508.
10. Komarov PG, Komarova EA, Kondratov RV, Christov-Tselkov K, Coon JS, Chernov MV, and Gudkov AV (1999) A chemical inhibitor of p53 that protects mice from the side effects of cancer therapy. Science (Wash DC) 285:1733-1737.
11. Levine AJ (1997) p53, the cellular gatekeeper for growth and division. Cell 88:323-331.
12. Liebermann DA, Hoffman B, and Steinman RA (1995) Molecular controls of growth arrest and apoptosis: p53-dependent and independent pathways. Oncogene 11:199-210.
13. Liu X, Chua CC, Gao J, Chen Z, Landy CL, Hamdy R, and Chua BH (2004) Pifithrin-alpha protects against doxorubicin-induced apoptosis and acute cardiotoxicity in mice. Am J Physiol 286:H933-H939.
14. Martin DP, Schmidt RE, DiStefano PS, Lowry OH, Carter JG, and Johnson EM Jr (1988) Inhibitors of protein synthesis and RNA synthesis prevent neuronal death caused by nerve growth factor deprivation. J Cell Biol 106:829-844.
15. Mattson MP and Furukawa K (1997) Anti-apoptotic actions of cycloheximide: blockade of programmed cell death or induction of programmed cell life? Apoptosis 2:257-264.
16. Miyashita T and Reed JC (1995) Tumor suppressor p53 is a direct transcriptional activator of the human bax gene. Cell 80:293-299.
17. Murphy PJ, Galigniana MD, Morishima Y, Harrell JM, Kwok RP, Ljungman M, and Pratt WB (2004) Pifithrin-alpha inhibits p53 signaling after interaction of the tumor suppressor protein with hsp90 and its nuclear translocation. J Biol Chem 279:30195-30201.
18. Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T, Tokino T, Taniguchi T, and Tanaka N (2000a) Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science (Wash DC) 288:1053-1058.
19. AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell 102:849-862.
20. Owen-Schaub LB, Zhang W, Cusack JC, Angelo LS, Santee SM, Fujiwara T, Roth JA, Deisseroth AB, Zhang WW, Kruzel E, et al. (1995) Wild-type human p53 and a temperature-sensitive mutant induce Fas/APO-1 expression. Mol Cell Biol 15:3032-3040.
21. Pittman RN, Wang S, DiBenedetto AJ, and Mills JC (1993) A system for characterizing cellular and molecular events in programmed neuronal cell death. J Neurosci 13:3669-3680.
22. Polyak K, Xia Y, Zweier JL, Kinzler KW, and Vogelstein B (1997) A model for p53-induced apoptosis. Nature (Lond) 389:300-305.
23. Ratan RR, Murphy TH, and Baraban JM (1994) Macromolecular synthesis inhibitors prevent oxidative stress-induced apoptosis in embryonic cortical neurons by shunting cysteine from protein synthesis to glutathione. J Neurosci 14:4385-4392.
24. Sǎanchez A, Alvarez AM, Benito M, and Fabregat I (1997) Cycloheximide prevents apoptosis, reactive oxygen species production, and glutathione depletion induced by transforming growth factor beta in fetal rat hepatocytes in primary culture. Hepatology 26:935-943.
25. Satoh T, Sakai N, Enokido Y, Uchiyama Y, and Hatanaka H (1996) Survival factor-insensitive generation of reactive oxygen species induced by serum deprivation in neuronal cells. Brain Res 733:9-14.
26. Schamberger CJ, Gerner C, and Cerni C (2005) Caspase-9 plays a marginal role in serum starvation-induced apoptosis. Exp Cell Res 302:115-128.
27. Serghini R, Froissard P, Sola B, and Duval D (1994) Cycloheximide and actinomycin D block the toxic effect of glutamic acid on PC12 cells. Neuroreport 6:29-32.
28. Steller H (1995) Mechanisms and genes of cellular suicide. Science (Wash DC) 267:1445-1449.
29. van der Valk J, Mellor D, Brands R, Fischer R, Gruber F, Gstraunthaler G, Hellebrekers L, Hyllner J, Jonker FH, Prieto P, et al. (2004) The humane collection of fetal bovine serum and possibilities for serum-free cell and tissue culture. Toxicol In Vitro 18:1-12.
30. Vogelstein B and Kinzler KW (1992) p53 function and dysfunction. Cell 70:523-526.
31. Wyllie AH, Morris RG, Smith AL, and Dunlop D (1984) Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis. J Pathol 142:67-77.
32. Yonish-Rouach E, Deguin V, Zaitchouk T, Breugnot C, Mishal Z, Jenkins JR, and May E (1995) Transcriptional activation plays a role in the induction of apoptosis by transiently transfected wild-type p53. Oncogene 11:2197-2205.
33. Yu AC, Yung HW, Hui MH, Lau LT, Chen XQ, and Collins RA (2003) Cycloheximide and actinomycin D delay death and affect bcl-2, bax, and Ice gene expression in astrocytes under in vitro ischemia. J Neurosci Res 74:318-325.
34. Yu J, Zhang L, Hwang PM, Kinzler KW, and Vogelstein B (2001) PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell 7:673-682.