Selective adsorption of large proteins on highly activated IMAC supports in the presence of high imidazole concentrations: Purification, reversible immobilization and stabilization of thermophilic α- and β-galactosidases

Enzyme and Microbial Technology

Volumn 40, Issue 2, 2007, Pages 242-248

  Pessela, Benevides C C ^b   Mateo, Cesar ^a   Filho, Miguel ^a   Carrascosa, Alfonso ^b   Fernández Lafuente, Roberto ^a   Guisan, José M ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

^b INSTITUTO DE FERMENTACIONES INDUSTRIALES CSIC   (Spain)

Author keywords

Multimeric proteins; Multipoint adsorption; Selective adsorption; Selective immobilization; Thermophilic proteins

Indexed keywords

ADSORPTION; AFFINITY CHROMATOGRAPHY; CHELATION; CONCENTRATION (PROCESS); PURIFICATION;

MULTIMERIC PROTEINS; MULTIPOINT ADSORPTION; SELECTIVE ADSORPTION; SELECTIVE IMMOBILIZATION; THERMOPHILIC PROTEINS;

PROTEINS;

ALPHA GALACTOSIDASE; BETA GALACTOSIDASE; IMIDAZOLE;

ADSORPTION KINETICS; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; ENZYME STABILITY; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; PROTEIN DETERMINATION; PROTEIN EXPRESSION; THERMOPHILIC BACTERIUM; THERMOSTABILITY; THERMUS;

ESCHERICHIA COLI; THERMUS SP.;

EID: 33751164160     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2006.04.019     Document Type: Article

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