Cloning, expression and rapid purification of active recombinant mycothiol ligase as B1 immunoglobulin binding domain of streptococcal protein G, glutathione-S-transferase and maltose binding protein fusion proteins in Mycobacterium smegmatis

Protein Expression and Purification

Volumn 50, Issue 1, 2006, Pages 128-136

  Gutierrez Lugo, Maria Teresa ^a   Newton, Gerald L ^b   Fahey, Robert C ^b   Bewley, Carole A ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Affinity purification; Complementation; Fusion proteins; MshC; Mycobacterium tuberculosis; Mycothiol; Mycothiol ligase; Shuttle vector

Indexed keywords

BACTERIAL PROTEIN; CARRIER PROTEIN; CYSTEINE; GLUTATHIONE TRANSFERASE; GLYCOPEPTIDE; IGG FC BINDING PROTEIN, STREPTOCOCCUS; IGG FC-BINDING PROTEIN, STREPTOCOCCUS; IMMUNOGLOBULIN; INOSITOL; LIGASE; MALTOSE BINDING PROTEIN; MALTOSE-BINDING PROTEIN; MYCOTHIOL; RECOMBINANT PROTEIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; CONFORMATION; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR CLONING; MYCOBACTERIUM SMEGMATIS; PROTEIN BINDING; TIME;

BACTERIAL PROTEINS; CARBOHYDRATE CONFORMATION; CARRIER PROTEINS; CLONING, MOLECULAR; CYSTEINE; GLUTATHIONE TRANSFERASE; GLYCOPEPTIDES; IMMUNOGLOBULINS; INOSITOL; LIGASES; MYCOBACTERIUM SMEGMATIS; PROTEIN BINDING; RECOMBINANT PROTEINS; TIME FACTORS;

ACTINOBACTERIA; ACTINOBACTERIA (CLASS); CORYNEBACTERINEAE; ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS;

EID: 33751050875     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.07.005     Document Type: Article

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