Effects of mutations in the substrate-binding domain of poly[(R)-3-hydroxybutyrate] (PHB) depolymerase from Ralstonia pickettii T1 on PHB degradation

Applied and Environmental Microbiology

Volumn 72, Issue 11, 2006, Pages 7331-7338

  Hiraishi, Tomohiro ^a   Hirahara, Yoko ^a   Doi, Yoshiharu ^a   Maeda, Mizuo ^a   Taguchi, Seiichi ^b  

^a RIKEN   (Japan)

^b HOKKAIDO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ADSORPTION; AMINO ACIDS; BACTERIA; BINDING ENERGY; BIODEGRADATION; GENES; MUTAGENESIS; SUBSTRATES; THROUGHPUT;

HYDROPHOBIC RESIDUES; MUTANT ENZYMES; POLY (R)-3-HYDROXYBUTYRATE (PHB); SUBSTRATE BINDING DOMAIN (SBD);

ENZYMES;

ALANINE; BACTERIAL ENZYME; HYDROXYL GROUP; LEUCINE; MUTANT PROTEIN; POLY(3 HYDROXYBUTYRATE) DEPOLYMERASE; POLY(3 HYDROXYBUTYRIC ACID); SERINE; TYROSINE; UNCLASSIFIED DRUG; VALINE;

ADSORPTION; AMINO ACID; BACTERIUM; BIOASSAY; DEGRADATION; ENZYME ACTIVITY; KINETICS; MUTATION;

ADSORPTION; AMINO ACID ANALYSIS; AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIUM MUTANT; BINDING AFFINITY; DEGRADATION KINETICS; ENZYMATIC DEGRADATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME MODIFICATION; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; GENE MUTATION; GENETIC ANALYSIS; HYDROGEN BOND; HYDROLYSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN DOMAIN; RALSTONIA; RALSTONIA PICKETTII; WILD TYPE;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CARBOXYLIC ESTER HYDROLASES; HYDROXYBUTYRATES; MOLECULAR SEQUENCE DATA; MUTATION; POLYESTERS; POLYMERASE CHAIN REACTION; RALSTONIA PICKETTII; SUBSTRATE SPECIFICITY;

RALSTONIA PICKETTII;

EID: 33750990228     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01187-06     Document Type: Article

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