An LNA-based loss-of-function assay for micro-RNAs

Biomedicine and Pharmacotherapy

Volumn 60, Issue 9, 2006, Pages 633-638

  Naguibneva, I ^a   Ameyar Zazoua, M ^a   Nonne, N ^a   Polesskaya, A ^a   Ait Si Ali, S ^a   Groisman, R ^a   Souidi, M ^a   Pritchard, L L ^a   Harel Bellan, A ^a  

^a CNRS   (France)

Author keywords

Antisense; Locked nucleic acid (LNA); Micro RNAs

Indexed keywords

CELLS, CULTURED; HUMANS; MICRORNAS; OLIGONUCLEOTIDES, ANTISENSE;

EID: 33750987359     PISSN: 07533322     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biopha.2006.07.078     Document Type: Article

References (12)

1. Ambros V. microRNAs: tiny regulators with great potential. Cell 107 (2001) 823-826
2. Boutla A., Delidakis C., and Tabler M. Developmental defects by antisense-mediated inactivation of micro-RNAs 2 and 13 in Drosophila and the identification of putative target genes. Nucleic Acids Res. 31 (2003) 4973-4980
3. Chan J.A., Krichevsky A.M., and Kosik K.S. MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. Cancer Res. 65 (2005) 6029-6033
4. Hutvagner G., Simard M.J., Mello C., and Zamore P. Sequence-Specific Inhibition of Small RNA Function. PLoS Biol. 2 (2004) 1-11
5. Koshkin A.A., Nielsen P., Meldgaard M., Rajwanshi V.K., Singh S.K., and Wengel J. LNA (Locked Nucleic Acid): An RNA mimic forming exceedingly stable LNA:LNA duplexes. J. Am. Chem. Soc. 120 (1998) 13252-13260
6. Kumar R., Singh S.K., Koshkin A.A., Rajwanshi V.K., Meldgaard M., and Wengel J. The first analogues of LNA (locked nucleic acids): phosphorothioate-LNA and 2'-thio-LNA. Bioorg. Med. Chem. Lett. 8 (1998) 2219-2222
7. Kurreck J., Wyszko E., Gillen C., and Erdmann V.A. Design of antisense oligonucleotides stabilized by locked nucleic acids. Nucleic Acids Res. 30 (2002) 1911-1918
8. Lee Y., Jeon K., Lee J.T., Kim S., and Kim V.N. MicroRNA maturation: stepwise processing and subcellular localization. EMBO J. 21 (2002) 4663-4670
9. Meister G., Landthaler M., Dorsett Y., and Tuschl T. Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing. RNA 10 (2004) 544-550
10. Wahlestedt C., Salmi P., Good L., Kela J., Johnsson T., Hokfelt T., et al. Potent and nontoxic antisense oligonucleotides containing locked nucleic acids. Proc. Natl. Acad. Sci. USA 97 (2000) 5633-5638
11. Suh M.R. Lee Y, Kim JY, Kim SK, Moon SH, Lee JY, Cha KY, Chung HM, Yoon HS, Moon SY, Kim VN, Kim KS. Human embryonic stem cells express a unique set of microRNAs. Dev. Biol. 270 (2004) 488-498
12. Naguibneva I., Ameyar-Zazoua M., Polesskaya A., Ait-Si-Ali S., Groisman R., Souidi M., et al. The microRNA miR-181 targets the homeobox protein Hox-A11 during mammalian myoblast differentiation. Nat. Cell Biol. 8 (2006) 278-284