Conductance and selectivity fluctuations in D127 mutants of the bacterial porin OmpF

European Biophysics Journal

Volumn 36, Issue 1, 2006, Pages 13-22

  Miedema, Henk ^a   Vrouenraets, Maarten ^a   Wierenga, Jenny ^a   Eisenberg, Bob ^b   Schirmer, Tilman ^c   Baslé, Arnaud ^c   Meijberg, Wim ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b RUSH UNIVERSITY MEDICAL CENTER   (United States)

^c UNIVERSITY OF BASEL   (Switzerland)

Author keywords

Current fluctuations; Ion selectivity; OmpF D127 mutant; OmpF Loop L3

Indexed keywords

ASPARTIC ACID; LYSINE; OUTER MEMBRANE PROTEIN F; PROTON;

ARTICLE; CONDUCTANCE; ELECTRIC FIELD; ELECTRICITY; ESCHERICHIA COLI; IONIZATION; PROTON TRANSPORT;

BACTERIAL OUTER MEMBRANE PROTEINS; BIOPHYSICS; ELECTROPHYSIOLOGY; ELECTROSTATICS; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; ION CHANNEL GATING; IONS; LYSINE; MEMBRANE PROTEINS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; PORINS; PROTEIN STRUCTURE, SECONDARY;

BACTERIA (MICROORGANISMS);

EID: 33750932922     PISSN: 01757571     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00249-006-0084-4     Document Type: Article

References (35)

1. Alcaraz A, Nestorovich EM, Aguilella-Arzo M, Aguilella VM, Bezrukov SM (2004) Salting out the ionic selectivity of a wide channel: the asymmetry of OmpF. Biophys J 87:943-957
2. Bainbridge G, Gocke L, Lakey JH (1998) Voltage gating is a fundamental feature of porin and toxin β-barrel membrane channels. FEBS Lett 431:305-308
3. Baslé A, Qutub R, Mehrazin M, Wibbenmeyer J, Delcour AH (2004) Deletions of single extracellular loops affect pH sensitivity, but not voltage dependence, of the Escherichia coli porin OmpF. Protein Eng 17:665-672
4. Bredin J, Saint N, Malléa M, Dé E, Molle G, Pagès J-M, Simonet V (2002) Alteration of pore properties of Escherichia coli OmpF induced by mutation of key residues in anti-loop 3 region. Biochem J 363:521-528
5. Cowan SW, Schirmer T, Rummel G, Steiert M, Ghosh R, Pauptit RA, Jansonius JN, Rosenbusch JP (1992) Crystal structures explain functional properties of two E. Coli porins. Nature 358:727-733
6. Cymes GD, Ni Y, Grosman C (2005) Probing ion channel pores one proton at a time. Nature 438:975-980
7. Danelon C, Suenaga A, Winterhalter M, Yamato L (2003) Molecular origin of the cation selectivity in OmpF porin: single channel conductances vs. free energy calculation. Biophys Chem 104:591-603
8. Delcour AH (2003) Solute uptake through general porins. Front Biosci 8:1055-1071
9. Eisenberg RS (1996a) Computing the field in proteins and channels. J Membr Biol 150:1-25
10. Eisenberg RS (1996b) Atomic biology, electrostatics and ionic channels. In: Elber R (eds) New developments and theoretical studies of proteins, World Scientific, Philadelphia, pp 269-357
11. Im W, Roux B (2002a) Ions and counterions in a biological channel a molecular dynamics simulation of OmpF porin: from Escherichia coli in an explicit membrane with 1 M KCl aqueous salt solution. J Mol Biol 319:1177-1197
12. Im W, Roux B (2002b) Ion permeation and selectivity of OmpF porin: a theoretical study based on molecular dynamics, Brownian dynamics, and continuum electrodiffusion theory. J Mol Biol 322:851-869
13. Karshikoff A, Spassov V, Cowan SW, Ladenstein R, Schirmer T (1994) Electrostatic properties of two porin channels from Escherichia coli. J Mol Biol 240:372-384
14. Klebba PE, Newton SMC (1998) Mechanisms of solute transport through outer membrane porins: burning down the house. Curr Opion Microbiol 1:238-248
15. Liu N, Delcour AH (1998) The spontaneous gating activity of OmpC porin is affected by mutations of a putative hydrogen bond network or of a salt bridge between the L3 loop and the barrel. Protein Eng 11:797-802
16. Liu N, Samartzidou H, Lee KW, Briggs JM, Delcour AH (2000) Effects of pore mutations and permeant ion concentration on the spontaneous gating activity of OmpC porin. Protein Eng 13:491-500
17. 2+ channels. Biophys J 87:3137-3147
18. Nestorovich EM, Rostovtseva TK, Bezrukov SM (2003) Residue ionization and ion transport through OmpF channels. Biophys J 85:3718-3729
19. Phale PS, Schirmer T, Prilipov A, Lou K-L, Hardmeyer A, Rosenbusch JP (1997) Voltage gating of Escherichia coli porin channels: role of the constriction zone. Proc Natl Acad Sci USA 94:6741-6745
20. Phale PS, Philippsen A, Widmer C, Pahe VP, Rosenbusch JP, Schirmer T (2001) Role of charged residues at the OmpF porin channel constriction probed by mutagenesis and simulation. Biochemistry 40:6319-6325
21. Philippsen A, Im W, Engel A, Schirmer T, Roux B, Müller DJ (2002) Imaging the electrostatic potential of transmembrane channels: atomic probe microscopy of OmpF porin. Biophys J 82:1667-1676
22. Robertson KM, Tieleman DP (2002) Molecular basis of voltage gating of OmpF porin. Biochem Cell Biol 80:517-523
23. Saint N, Lou K-L, Widmer C, Luckey M, Schirmer T, Rosenbusch JP (1996) Structural and functional characterization of OmpF porin mutants selected for larger pore size. J Biol Chem 271:20676-20680
24. Saxena K, Drosou V, Maier E, Benz R, Ludwig B (1999) Ion selectivity reversal and induction of voltage-gating by site-directed mutations in the Paracoccus denitrificans porin. Biochemistry 38:2206-2212
25. Schindler H, Rosenbusch JP (1978) Matrix protein from Escherichia coli outer membranes forms voltage-controlled channels in lipid bilayers. Proc Natl Acad Sci USA 75:3751-3755
26. Schirmer T (1998) General and specific porins from bacterial outer membranes. J Struct Biol 121:101-109
27. Schirmer T, Phale PS (1999) Brownian dynamics simulation of ion flow through porin channels. J Mol Biol 294:1159-1167
28. Schulz GE (2002) The structure of bacterial outer membrane proteins. Biochem Biophys Acta 1565:308-317
29. Tieleman DP, Berendsen HJC (1998) A molecular dynamics study of the pores formed by Escherichia coli OmpF porin in a fully hydrated plamitoyloleoylphosphatidylcholine bilayer. Biophys J 74:2786-2801
30. Todt JC, McGroarty EJ (1992) Involvement of Histidine-21 in the pH-induced switch in porin channel size. Biochemistry 31:10479-10482
31. Todt JC, Roqueand WJ, McGroarty EJ (1992) Effects of pH on bacterial porin function. Biochemistry 31:10471-10478
32. Van Gelder PN, Saint N, Phale PS, Eppens EF, Prilipov A, van Boxtel R, Rosenbusch JP (1997) Voltage sensing in the PhoE and OmpF outer membrane porins of Escherichia coli: role of charged residues. J Mol Biol 269:468-472
33. Varma S, Jakobsson E (2004) Ionization states of residues in OmpF and mutants: effects of dielectric constant and interactions between residues and ionic strength. Biophys J 86:690-704
34. Varma S, Chiu S-W, Jakobsson E (2006) The influence of amino acid protonation states on molecular dynamics simulations of the bacterial porin OmpF. Biophys J 90:112-123
35. Vrouenraets M, Wierenga J, Meijberg W, Miedema H (2006) Chemical modification of the bacterial porin OmpF: gain of selectivity by volume reduction. Biophys J 90:1202-1211