The Ligand-Independent Translocation Assay: An Enabling Technology for Screening Orphan G Protein-Coupled Receptors by Arrestin Recruitment

Methods in Enzymology

Volumn 414, Issue , 2006, Pages 50-63

  Oakley, Robert H ^a   Hudson, Christine C ^a   Sjaastad, Michael D ^a   Loomis, Carson R ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

G PROTEIN COUPLED RECEPTOR; G PROTEIN COUPLED RECEPTOR KINASE; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; RETINA S ANTIGEN;

BACULOVIRUS; BINDING AFFINITY; CELL STRAIN HEK293; CELLULAR DISTRIBUTION; DRUG TARGETING; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; HIGH THROUGHPUT SCREENING; HUMAN; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN MODIFICATION; PROTEIN PHOSPHORYLATION; QUALITY CONTROL; QUANTITATIVE ANALYSIS; RELIABILITY; REVIEW;

EID: 33750819442     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(06)14004-5     Document Type: Chapter

References (19)

1. Cao W., Luttrell L.M., Medvedev A.V., Pierce K.L., Daniel K.W., Dixon T.M., Lefkowitz R.J., and Collins S. Direct binding of activated c-Src to the beta 3-adrenergic receptor is required for MAP kinase activation. J. Biol. Chem. 275 (2000) 38131-38134
2. Claing A., Laporte S.A., Caron M.G., and Lefkowitz R.J. Endocytosis of G protein-coupled receptors: Roles of G protein-coupled receptor kinases and ss-arrestin proteins. Prog. Neurobiol. 66 (2002) 61-79
3. Comley J. High content screening: Emerging importance of novel reagents/probes and pathway analysis. Drug Disc. World 6 (2005) 31-53
4. Ferguson S.S. Evolving concepts in G protein-coupled receptor endocytosis: The role in receptor desensitization and signaling. Pharmacol. Rev. 53 (2001) 1-24
5. Galliera E., Jala V.R., Trent J.O., Bonecchi R., Signorelli P., Lefkowitz R.J., Mantovani A., Locati M., and Haribabu B. Beta-Arrestin-dependent constitutive internalization of the human chemokine decoy receptor D6. J. Biol. Chem. 279 (2004) 25590-25597
6. Howard A.D., McAllister G., Feighner S.D., Liu Q., Nargund R.P., Van der Ploeg L.H., and Patchett A.A. Orphan G-protein-coupled receptors and natural ligand discovery. Trends Pharmacol. Sci. 22 (2001) 132-140
7. Inglese J., Koch W.J., Caron M.G., and Lefkowitz R.J. Isoprenylation in regulation of signal transduction by G-protein-coupled receptor kinases. Nature 359 (1992) 147-150
8. Marion S., Weiner D.M., and Caron M.G. RNA editing induces variation in desensitization and trafficking of 5-hydroxytryptamine 2c receptor isoforms. J. Biol. Chem. 279 (2004) 2945-2954
9. Miller W.E., Houtz D.A., Nelson C.D., Kolattukudy P.E., and Lefkowitz R.J. G-protein-coupled receptor (GPCR) kinase phosphorylation and beta-arrestin recruitment regulate the constitutive signaling activity of the human cytomegalovirus US28 GPCR. J. Biol. Chem. 278 (2003) 21663-21671
10. Oakley R.H., Barak L.S., and Caron M.G. Real time imaging of GPCR-mediated arrestin translocation as a strategy to evaluate receptor-protein interactions. In: George S.R., and O'Dowd B.F. (Eds). "G Protein Coupled Receptor-Protein Interactions" (2005), Wiley, New York 53-80
11. Oakley R.H., Cowan C.L., Hudson C.C., and Loomis C.R. Transfluor provides a universal cell-based assay for screening G protein-coupled receptors. In: Minor L. (Ed). "Handbook of Assay Development in Drug Discovery" (2006), Dekker, New York 435-457
12. Oakley R.H., Hudson C.C., Cruickshank R.D., Meyers D.M., Payne Jr. R.E., Rhem S.M., and Loomis C.R. The cellular distribution of fluorescently labeled arrestins provides a robust, sensitive, and universal assay for screening G protein-coupled receptors. Assay Drug Dev. Technol. 1 (2002) 21-30
13. Oakley R.H., Laporte S.A., Holt J.A., Barak L.S., and Caron M.G. Association of beta-arrestin with G protein-coupled receptors during clathrin-mediated endocytosis dictates the profile of receptor resensitization. J. Biol. Chem. 274 (1999) 32248-32257
14. Oakley R.H., Laporte S.A., Holt J.A., Barak L.S., and Caron M.G. Molecular determinants underlying the formation of stable intracellular G protein-coupled receptor-beta-arrestin complexes after receptor endocytosis. J. Biol. Chem. 276 (2001) 19452-19460
15. Oakley R.H., Laporte S.A., Holt J.A., Caron M.G., and Barak L.S. Differential affinities of visual arrestin, beta arrestin1, and beta arrestin2 for G protein-coupled receptors delineate two major classes of receptors. J. Biol. Chem. 275 (2000) 17201-17210
16. Pitcher J.A., Freedman N.J., and Lefkowitz R.J. G protein-coupled receptor kinases. Annu. Rev. Biochem. 67 (1998) 653-692
17. Samama P., Cotecchia S., Costa T., and Lefkowitz R.J. A mutation-induced activated state of the beta 2-adrenergic receptor: Extending the ternary complex model. J. Biol. Chem. 268 (1993) 4625-4636
18. Wise A., Jupe S.C., and Rees S. The identification of ligands at orphan G-protein coupled receptors. Annu. Rev. Pharmacol. Toxicol. 44 (2004) 43-66
19. Zhang J.H., Chung T.D., and Oldenburg K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen 4 (1999) 67-73