Immunolocalization of duodenal cytochrome B: A relationship with circulating markers of iron status

European Journal of Clinical Investigation

Volumn 36, Issue 12, 2006, Pages 890-898

  Li, A C Y ^a,b   Warley, A ^a   Thoree, V ^a   Simpson, R J ^c   Mckie, A T ^c   Kodjabashia, K ^a   Thompson, R P H ^a   Powell, J J ^d,e  

^a ST THOMAS HOSPITAL   (United Kingdom)

^b WORTHING HOSPITAL   (United Kingdom)

^c KING'S COLLEGE LONDON   (United Kingdom)

^d MRC HUMAN NUTRITION RESEARCH   (United Kingdom)

^e MEDICAL RESEARCH COUNCIL   (United Kingdom)

Author keywords

Dcytb; Electron microscopy; Ferritin; Immunogold; Immunohistochemistry; Iron

Indexed keywords

BIOLOGICAL MARKER; CYTOCHROME B; IRON; IRON DERIVATIVE; IRON REDUCTASE; UNCLASSIFIED DRUG;

ANTIBODY LABELING; ARTICLE; BLOOD SAMPLING; CLINICAL ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CORRELATION ANALYSIS; CYTOPLASM; DUODENUM; DUODENUM BIOPSY; ELECTRON MICROSCOPY; GASTROINTESTINAL ENDOSCOPY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOGOLD LABELING; IMMUNOHISTOCHEMISTRY; IMMUNOLOCALIZATION; INTESTINE BRUSH BORDER; INTESTINE CELL; IRON DEFICIENCY; MICROSCOPY; MICROVILLUS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION;

BIOLOGICAL MARKERS; CYTOCHROME B GROUP; DUODENUM; FERRITINS; HUMANS; IMMUNOHISTOCHEMISTRY; INTESTINAL ABSORPTION; INTESTINAL MUCOSA; IRON; MICROSCOPY, ELECTRON;

EID: 33750720942     PISSN: 00142972     EISSN: 13652362     Source Type: Journal    
DOI: 10.1111/j.1365-2362.2006.01732.x     Document Type: Article

References (31)

1. Fleming MD, Trenor CC III, Su MA, Foernzler D, Beier DR, Dietrich WF et al. Microcytic anaemia mice have a mutation in Nramp2, a candidate iron transporter gene. Nat Genet 1997;16:383-6.
2. McKie AT, Marciani P, Rolfs A, Brennan K, Wehr K, Barrow D et al. A novel duodenal iron-regulated transporter, IREG1, implicated in the basolateral transfer of iron to the circulation. Mol Cell 2000;5:299-309.
3. McKie AT, Barrow D, Latunde-Dada GO, Rolfs A, Sager G, Mudaly E et al. An iron-regulated ferric reductase associated with the absorption of dietary iron. Science 2001;291:1755-9.
4. Abboud S, Haile DJ. A novel mammalian iron-regulated protein involved in intracellular iron metabolism. J Biol Chem 2000;275:19906-12.
5. Zoller H, Koch RO, Theurl I, Obrist P, Pietrangelo A, Montosi G et al. Expression of the duodenal iron transporters divalent-metal transporter 1 and ferroportin 1 in iron deficiency and iron overload. Gastroenterology 2001;120:1412-9.
6. Latunde-Dada GO, Van der Westhuizen J, Vulpe CD, Anderson GJ, Simpson RJ, McKie AT. Molecular and functional roles of duodenal cytochrome B (DcytB) in iron metabolism. Blood Cells Mol Dis 2002;29:356-60.
7. Anderson GJ, Powell LW. Of metals, mice and men: what animal models can teach us about body iron loading. J Clin Invest 2000;105:1185-6.
8. Walker BL, Tiong JW, Jefferies WA. Iron metabolism in mammalian cells. Int Rev Cytol 2001;211:241-78.
9. Chasteen ND, Harrison PM. Mineralization in ferritin: an efficient means of iron storage. J Struct Biol 1999;126:182-94.
10. Bomford A, Conlon-Hollingshead C, Munro HN. Adaptive responses of rat tissue isoferritins to iron administration. Changes in sub-unit synthesis, isoferritin abundance, and capacity for iron storage. J Biol Chem 1981;256:948-55.
11. Liu X, Theil EC. Ferritin reactions: direct identification of the site for the diferric peroxide reaction intermediate. Proc Natl Acad Sci USA 2004;101:8557-62.
12. Crichton RR, Wilmet S, Legssyer R, Ward RJ. Molecular and cellular mechanisms of iron homeostasis and toxicity in mammalian cells. J Inorg Biochem 2002;91:9-18.
13. Pantopoulos K. Iron metabolism and the IRE/IRP regulatory system: an update. Ann N Y Acad Sci 2004;1012:1-13.
14. Brutsaert TD, Hernandez-Cordero S, Rivera J, Viola T, Hughes G, Haas JD. Iron supplementation improves progressive fatigue resistance during dynamic knee extensor exercise in iron-depleted, non-anemic women. Am J Clin Nutr 2003;77:441-8.
15. Suedekum NA, Dimeff RJ. Iron and the athlete. Curr Sports Med Rep 2005;4:199-202.
16. Fleming RE. Advances in understanding the molecular basis for the regulation of dietary iron absorption. Curr Opin Gastroenterol 2005;21:201-6.
17. Luzzago A, Arosio P, Iacobello C, Ruggeri G, Capucci L, Brocchi E et al. Immunochemical characterization of human liver and heart ferritins with monoclonal antibodies. Biochim Biophys Acta 1986;872:61-71.
18. Ruggeri G, Santambrogio P, Bonfiglio F, Levi S, Bugari G, Verardi R et al. Antibodies for denatured human H-ferritin stain only reticuloendothelial cells within the bone marrow. Br J Haematol 1992;81:118-24.
19. Connor JR, Snyder BS, Arosio P, Loeffler DA, LeWitt P. A quantitative analysis of isoferritins in select regions of aged, Parkinsonian, and Alzheimer's diseased brains. J Neurochem 1995;65:717-24.
20. Pietrangelo A, Casalgrandi G, Quaglino D, Gualdi R, Conte D, Milani S et al. Duodenal ferritin synthesis in genetic hemochromatosis. Gastroenterology 1995;108:208-17.
21. Matkowskyj KA, Schonfeld D, Benya RV. Quantitative immunohistochemistry by measuring cumulative signal strength using commercially available software PhotoShop™ and Matlab™. J Histochem Cytochem 2000;48:303-12.
22. Tokuyasu KT. Application of cryo-ultra-microtomy to immunocytochemistry. J Microsc 1986;143:139-49.
23. Mayhew TM, Lucocq JM, Griffiths G. Relative labelling index: a novel stereological approach to test for non-random immunogold labelling of organelles and membranes on transmission electron microscopy thin sections. J Microsc 2002;205:153-64.
24. Ruifrok AC. Quantification of immunohistochemical staining by color translation and automated thresholding. Anal Quant Cytol Histol 1997;19:107-13.
25. Cairo G, Pietrangelo A. Iron regulatory proteins in pathobiology. Biochem J 2000;352 (Part 2):241-50.
26. Brittin GM, Raval D. Duodenal ferritin synthesis during iron absorption in the iron-deficient rat. J Lab Clin Med 1970;75:811-7.
27. Whittaker P, Skikne BS, Covell AM, Flowers C, Cooke A, Lynch SR et al. Duodenal iron proteins in idiopathic haemochromatosis. J Clin Invest 1989;83:261-7.
28. Francanzani AL, Fargion S, Romano R, Piperno A, Arosio P, Ruggeri G et al. Immunohistochemical evidence for a lack of ferritin in duodenal absorptive epithelial cells in idiopathic haemochromatosis. Gastroenterology 1989;96:1071-8.
29. Nicolas G, Bennoun M, Devaux I, Beaumont C, Grandchamp B, Kahn A et al. Lack of hepcidin gene expression and severe tissue iron overload in upstream stimulatory factor 2 (USF2) knockout mice. Proc Natl Acad Sci USA 2001;98:8780-5.
30. Lombard M, Chua E, O'Toole P. Regulation of intestinal non-haem iron absorption. Gut 1997;40:435-9.
31. Anderson GJ, Frazer DM. Recent advances in intestinal iron transport. Curr Gastroenterol Rep 2005;7:365-72.