Quantitative immunohistochemistry by measuring cumulative signal strength using commercially available software Photoshop and Matlab

Journal of Histochemistry and Cytochemistry

Volumn 48, Issue 2, 2000, Pages 303-311

  Matkowskyj, Kristina A ^a   Schonfeld, Dan ^a   Benya, Richard V ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

Bombesin; Cancer; Gastrin releasing peptide; Methods

Indexed keywords

BOMBESIN; GASTRIN RELEASING PEPTIDE;

ALGORITHM; ARTICLE; COMPUTER PROGRAM; CONTROLLED STUDY; DIGITAL FILTERING; HUMAN; HUMAN TISSUE; IMAGE PROCESSING; IMMUNOHISTOCHEMISTRY; PRIORITY JOURNAL; SIGNAL PROCESSING;

EID: 0034000849     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540004800216     Document Type: Article

References (15)

1. Carroll RE, Matkowskyj KA, Chakrabarti S, McDonald TJ, Benya RV (1999) Aberrant expression of gastrin-releasing peptide and its receptor by well differentiated colon cancers in humans. Am J Physiol 276:G655-G665
2. Fermin CD, Degraw S (1995) Color thresholding in video imaging. J Anat 186:469-481
3. Ferris HA, Carroll RE, Lorimer DL, Benya RV (1997) Location and characterization of the human GRP receptor expressed by gastrointestinal epithelial cells. Peptides 18:663-672
4. Goldlust EJ, Paczynksi RP, He YY, Hsu CY, Goldberg MP (1996) Automated measurement of infarct size with scanned images of triphenyltetrazolium chloride-stained rat brains. Stroke 27:1657-1662
5. Jain AK (1989) Fundamentals of Digital Image Processing. Englewood Cliffs, NJ, Prentice-Hall
6. Jasani B, Schmid KW (1993) Immunocytochemistry in Diagnostic Histopathology. 1st ed. Edinburgh, Churchill Livingstone
7. Kohlberger PD, Obermair A, Sliutz G, Heinzl H, Koelbl U, Breitenecker G, Gitsch G, Kainz C (1996) Quantitative immunohistochemistry of factor VIII-related antigen in breast carcinoma. Am J ClinPathol 105:705-710
8. Kroog G, Sainz E, Worland P, Akeson M, Benya RV, Jensen RT, Battey JF (1995) The gastrin-releasing peptide receptor is rapidly phosphorylated by a kinase other than protein kinase C after exposure to agonist. J Biol Chem 270:8217-8224
9. Kuyatt B, Reidy CA, Hui KY, Jordan WH (1993) Quantitation of smooth muscle proliferation in cutured aorta. A color image analysis method for the Macintosh. Anal Quant Cytol Histol 15:83-87
10. Lehr HA, Mankoff DA, Corwin D, Santeursanio G, Gown AM (1997) Application of Photoshop-based image analysis to quantification of hormone receptor expression in breast cancer. J Histochem Cytochem 45:1559-1565
11. Lehr HA, van der Loos CM, Teeling P, Gown AM (1999) Complete chromogen separation and analysis in double immunohistochemical stains using Photoshop-based image analysis. J Histochem Cytochem 47:119-125
12. Mosedale DH, Metcalfe JC, Grainger DJ (1996) Optimization of immunofluorescence methods by quantitative image analysis. J Histochem Cytochem 44:1043-1050
13. Ruifrok AC (1997) Quantification of immunohistochemical staining hy color translation and automated thresholding. Anal Quant Cytol Histol 19:107-113
14. Shi S-R, Chaiwun B, Young L, Cote RJ, Taylor CR (1993) Antigen retrieval technique utilizing citrate buffer or urea solution for immunohistochemical demonstration of androgen receptor in formalin-fixed paraffin section. J Histochem Cytochem 41:1599-1604
15. Shi S-R, Key ME, Kalra KL (1991) Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 39:741-748